云南高校图书馆联盟文献共享服务平台- 国家自然科学基金(50674101)

国家自然科学基金(50674101): 作品数：15被引量：54H指数：5; 导出分析报告; 相关作者：夏金兰张成桂彭安安邱冠周张瑞永更多>>; 相关机构：中南大学更多>>; 相关期刊：《Journal of Wuhan University of Technology(Materials Science)》《现代生物医学进展》《微生物学通报》《Transactions of Nonferrous Metals Society of China》更多>>; 相关主题：BIOLEACHINGACIDITHIOBACILLUS双向电泳RELATED嗜酸氧化亚铁硫杆菌更多>>; 相关领域：生物学冶金工程矿业工程轻工技术与工程更多>>

Differential expression of genes encoding sulfur metabolism-related periplasmic proteins of Acidithiobacillus ferrooxidans ATCC 23270: 《Transactions of Nonferrous Metals Society of China》2010年第12期2366-2370,共5页夏金兰张瑞永张倩武顺张成桂聂珍媛邱冠周; Projects(50674101,50974140)supported by the National Natural Science Foundation of China;Project(2010CB630902)supported by the National Basic Research Program of China; Reverse-transcription qualitative PCR(RT-qPCR)was used to analyze the changes in transcription levels of the sulfur metabolism-related periplasmic protein genes of Acidithiobacillus ferrooxidans ATCC 23270 grown on su...; 关键词：Acidithiobacillus ferrooxidans RT-QPCR periplasmic proteins

Extraction and Characterization of PHB from Acidiphilium cryptum DX1-1被引量：2: 《Journal of Wuhan University of Technology(Materials Science)》2010年第6期938-943,共6页徐爱玲夏金兰; Funded by the National Natural Science Foundation for Distinguished Group (No. 50621063);the National Natural Science Foundation of China (No.50674101);the National Innovation Experiment Program for University Students (No. 81053321); A new bacterial strain,was designated as strain Acidiphilium cryptum DX1-1,accumulates intracellular poly-β-hydroxybutyrate particles,four methods which have advantages and disadvantages for each were employed to ext...; 关键词：Acidiphilium cryptum PHB EXTRACTION CHARACTERIZATION

A new strain Leptospirillum ferriphilum YTW315 for bioleaching of metal sulfides ores被引量：1: 《中国有色金属学会会刊：英文版》2010年第1期135-141,共7页张瑞永夏金兰彭娟花张倩张成桂聂珍媛邱冠周; Projects(50621063, 50674101) supported by the National Natural Science Foundation of China;Project (2004CB619204) supported by the National Basic Research Program of China; A new strain named YTW315 was isolated from Dexing area using the double-layer culture technique. The morphological, biochemical and physiological characteristics of YTW315 were studied. Physiological investigation in...; 关键词：生物浸出硫化物矿石病毒 RRNA基因序列金属最佳生长条件系统发育分析生理生化

Bioleaching of chalcopyrite by UV-induced mutagenized Acidiphilium cryptum and Acidithiobacillus ferrooxidans被引量：8: 《Transactions of Nonferrous Metals Society of China》2010年第2期315-321,共7页徐爱玲夏金兰张帅杨宇聂珍媛邱冠周; Project(2010CB630902)supported by the National Basic Research Program of China;Projects(50674101,50974140)supported by the National Natural Science Foundation of China; The original strains Acidithiobacillusferrooxidans GF and Acidiphilium cryptum DXI-1 were isolated from the drainage of some caves riched in chalcopyrite in Dexing Mine in Jiangxi Province of China. The optimum temper...; 关键词：Acidiphilium cryptum Acidithiobacillusferrooxidans UV-induced mutagenesis bioleaehing chaleopyrite

Isolation and characterization of acidophilic bacterium from Dongxiangshan Mine in Xinjiang Province, China: 《Journal of Central South University》2010年第1期50-55,共6页夏金兰张倩张瑞永彭娟花彭安安赵小娟聂珍媛邱冠周; Projects(50974140, 50674101) supported by the National Natural Science Foundation of China;Project(2010CB630902) supported by the National Basic Research Program of China; One bioleaching bacterium, named as strain DXS, was isolated from acid mine drainages （AMDs） of Dongxiangshan Mine of Hami, Xinjiang Province, China. The strain DXS is gram-negative and rod-shaped with a size of （0...; 关键词：.4cidithiobacillusferrooxidans BIOLEACHING iro and tth gene 16S rRNA

粘质沙雷氏菌XJ-01几丁质酶合成条件的优化被引量：1: 《现代生物医学进展》2009年第17期3208-3211,3250,共5页夏金兰熊晶刘可可张瑞永李红燕谭建锡彭安安聂珍媛; 国家自然基金创新研究群体科学基金项目(50621063);国家自然科学基金项目(50674101); 目的:对粘质沙雷氏菌(Serratia marcescens) XJ-01几丁质酶合成条件优化。方法:研究了碳源、氮源、温度、pH等单个因素对该菌几丁质酶合成的影响,并通过正交实验确定了该菌的最适酶合成条件。结果:该菌几丁质酶最优合成条件为:胶体几丁...; 关键词：粘质沙雷氏菌几丁质酶优化

嗜酸氧化亚铁硫杆菌ATCC23270周质蛋白的选择性提取及差异表达被引量：3: 《中南大学学报（自然科学版）》2009年第4期845-850,共6页夏金兰王晶张倩张瑞永张成桂欧阳叙东邱冠周; 国家自然科学基金资助项目(50674101);"973"计划项目(2004CB619201);国家自然基金创新研究群体科学基金资助项目(50621063); 通过考察萃取时间、高渗液与低渗液的摩尔比以及总的渗透液体积对细胞质苹果酸脱氢酶的比活性(相对于细胞蛋白质总量)的影响,建立选择性提取Acidithiobacillus ferrooxidans ATCC23270周质蛋白的渗透休克实验方法;结合透射电镜和双向电...; 关键词：ACIDITHIOBACILLUS ferrooxidans 周质蛋白双向电泳苹果酸脱氢酶活性渗透休克法

Purification and characterization of extracellular chitinase from a novel strain Aspergillus fumigatus CS-01: 《Journal of Central South University》2009年第4期552-557,共6页夏金兰熊晶徐涛张成桂张瑞永张倩武顺邱冠周; Projects(50621063, 50674101) supported by the National Natural Science Foundation of China; Twelve samples derived from different locations in south central area of China are treated by enrichment and spread-plate technique for initial screening. Seven chitinase-producing strains are isolated. The chitinase ...; 关键词：ASPERGILLUSFUMIGATUS CHITINASE PURIFICATION

大肠杆菌外膜蛋白的分离及其双向电泳图谱的建立被引量：10: 《现代生物医学进展》2009年第2期201-204,215,共5页夏金兰欧阳叙东张成桂彭安安王晶; 国家973计划"微生物冶金的基础研究"项目(2004CB619201);国家自然基金创新研究群体科学基金项目(50621063);国家自然科学基金项目(50674101); 本文利用温度诱导的两相分离萃取技术选择性分离未经机械破碎的大肠杆菌细胞外膜蛋白,研究Triton X-114的浓度及处理时间对提取外膜蛋白的影响。实验结果表明,Triton X-114的使用浓度和作用时间均显著影响外膜蛋白的提取效率。SDS-PAGE...; 关键词：大肠杆菌外膜蛋白 TritonX-114 双向电泳

Identification and fermentation optimization of protopectinase-overproducing strain Aspergillus niger CD-01 for pectin production被引量：4: 《Journal of Central South University》2009年第1期53-60,共8页夏金兰孟浩王润民张成桂熊晶聂珍媛邱冠周; Projects(50621063, 50674101) supported by the National Natural Science Foundation of China; In order to solve the citrus peel resource waste problem protopectinase-overproducing strain CD-01 for pectin production and minimize the drawbacks of chemical extraction of pectin, a was isolated from a pit soil dump...; 关键词：Aspergilllus niger pectin production protopectinase citrus peel fermentation optimization

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