This work was supported by the grants from the National Natural Science Foundation of China (30770045), National Basic Research Program (2011CBA00801), and Chinese Academy of Sciences Project (KSCX2-EW-G-13).
The genus of Nocardiopsis is a new source of antibiotics, chemicals, and enzymes. Here we reported the development of a vector and host system in moderately halophilic Nocardiopsis via an oriT-mediated conjugation. By...
This work was supported by grants from National Natural Science Foundation of China (30770045 and 30870067), National '863' project (2007AA021503) and the Chinese Academy of Sciences project (KSCX2-YW-G-069) to Z.Q.
The standard gene disruption and replacement to delete the actinorhodin biosynthetic gene cluster (Act) in Streptomyces coelicolor was inefficient, and the polymerase chain reaction-targeting of the cosmid could eff...