国家自然科学基金(30170014)

作品数:6被引量:17H指数:2
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相关作者:朱兴国刘丽仇润祥唐国敏刘谨更多>>
相关机构:中国科学院山东大学更多>>
相关期刊:《Science China(Life Sciences)》《Progress in Natural Science:Materials International》《生物工程学报》《自然科学进展》更多>>
相关主题:丝状真菌黑曲霉糖化酶GLUCOAMYLASEASPERGILLUS_NIGER蛋白更多>>
相关领域:生物学医药卫生更多>>
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Binding of trans -acting protein AngCP to the CCAAT-containing motifs in Aspergillus niger glaA promoter
《Progress in Natural Science:Materials International》2004年第4期338-343,共6页ZHUXingguo QIURunxiang LIULi TANGGuomin 
Supported by the National Natural Science Foundation of China (No. 30170014)
CCAAT-binding proteins AngCP1 and AngCP2 of Aspergillus niger binding to DC ( - 489--414 bp) and PC( - 390--345 bp) of A . niger glaA gene were respectively purified by 20 % - 40 % saturated ammonium sulfate, gel filt...
关键词:Aspergillus niger glucoamylase CCAAT-binding protein DNA-protein interaction protein purification. 
Identification of over-expressed genes in human renal cell carcinoma by combining suppression subtractive hybridi-zation and cDNA library array
《Science China(Life Sciences)》2004年第2期148-157,共10页AI Junkui1, ZHANG Zhiwen2, XIN Dianqi1, ZHU Hongjian1, YAN Quanjian3, XIN Zhongcheng1, NA Yanqun1 & GUO Yinglu1 1. Institute of Urology, Peking University & Department of Urology, First Hospital, Peking University, Beijing 100034, China 2. Department of Physiology, Peking University Health Science Center, Beijing 100083, China 3. Department of Urology, Xijing Hospital, the 4th Military Medical University, Xi抋n 710033, China 
co-supported by the Chinese Capital Scientific Development Fund for Medicine(ZD199803);Genomic Research Fund for Human Disease of Peking University.
To isolate the over-expressed genes in human renal cell carcinoma (RCC) and analyze its molecular basis of carcinogenesis, we used the mRNA from human RCC tissues as tester and that from the matched normal kidney tiss...
关键词:SUPPRESSION subtractive HYBRIDIZATION (SSH)  cDNA microarray  RENAL cell carcinoma (RCC)  gene  differential expression. 
The synergetic effects of two CCAAT boxes in Aspergillus niger glaA gene promoter on activation of PglaA transcription被引量:1
《Science China(Life Sciences)》2004年第2期139-147,共9页WANG H. M. 
supported by the National Natural Science Foundation of China(Grant No.30170014).
EMSA and footprinting analyses have revealed that the -489—-414 bp and the -390—-345 bp (designated DC and PC respectively) upstream of the Aspergillus niger T21 glaA gene were bound by one protein factor in the A. ...
关键词:Aspergillus NIGER glucoamylase  synergetic effect  mutation analysis  MULTI-COPY of CCAAT box  titration effect. 
黑曲霉糖化酶cis调控区上两个CCAAT框协同参与基因启动子的转录激活作用被引量:4
《中国科学(C辑)》2003年第6期495-504,共10页朱兴国 H.M.Wang 仇润祥 刘丽 董志扬 唐国敏 
国家自然科学基金资助项目(批准号:30170014)
已报道的EMSA和Footprinting试验表明黑曲霉Aspergillus niger T21糖化酶基因glaA启动子区-489~-414bp及-390~-345bp(分别简称为DC,PC)是与蛋白粗提液中同一蛋白因子结合,并均含有CCAAT五核苷酸的序列。对DC,PC在糖化酶表达调控中的...
关键词:黑曲霉糖化酶 转录调控 协同效应 突变分析 CCAAT多拷贝 滴定效应 基因启动子 激活作用 五核苷酸序列 
黑曲霉糖化酶启动子区CCAAT框与反式作用因子AngCP结合的分析被引量:1
《自然科学进展》2003年第12期1253-1258,共6页朱兴国 仇润祥 刘丽 唐国敏 
国家自然科学基金资助项目(批准号:30170014)
依次通过20%~40%饱和硫酸铵、凝胶过滤、肝素柱层析及DNA亲和层析纯化了黑曲霉糖化酶启动子区CCAAT框DC(-489 bp~-414 bp)结合蛋白(AngCP1)及CCAAT框PC(-390 bp~-345 bp)结合蛋白(AngCP2).凝胶过滤分析和SDS-PAGE结果表明AngCP1和A...
关键词:黑曲霉糖化酶 CCAAT结合蛋白 DNA-蛋白相互作用 蛋白纯化 反式作用因子 丝状真菌 基因表达 
丝状真菌表达分泌系统中受体菌的构建被引量:12
《生物工程学报》2002年第6期667-670,共4页刘丽 刘谨 仇润祥 朱兴国 唐国敏 
国家自然科学基金资助项目 (No.30 170 0 14 )~~
黑曲霉糖化酶高产菌株T2 1经紫外诱变后 ,通过酪蛋白平板和蛋白酶活性测定筛选出胞外酸性蛋白酶活力仅为原株 0 76 %的菌株A .nigerT2 1 2 0 1,其生长特性和产糖化酶活力与原株基本一致。利用原生质体 PEG法将含有报告基因vhb的表达...
关键词:丝状真菌 表达分泌系统 受体菌 构建 黑曲霉T21 蛋白酶缺陷株 VHB 
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