国家自然科学基金(30830015)

作品数:16被引量:76H指数:4
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相关作者:王广策牛建峰何林文王超周百成更多>>
相关机构:中国科学院中国科学院研究生院天津科技大学集美大学更多>>
相关期刊:《Science Bulletin》《安徽农业科学》《科学通报》《海洋科学》更多>>
相关主题:BRYOPSIS浒苔PROLIFERAULVA叶绿素荧光更多>>
相关领域:生物学农业科学轻工技术与工程更多>>
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Exploring valid internal-control genes in Porphyra yezoensis(Bangiaceae) during stress response conditions被引量:3
《Chinese Journal of Oceanology and Limnology》2014年第4期783-791,共9页王文磊 吴晓洁 王超 贾兆君 何林文 韦一凡 牛建峰 王广策 
Supported by the National Natural Science Foundation of China(Nos.41176134,30830015);the Prospective Joint Research Project of Jiangsu Province(No.BY2011188);the National Marine Public Welfare Research Project(Nos.201105008-2,201105023-7);the National Basic Research Program of China(973 Program)(No.2011CB411908)
To screen the stable expression genes related to the stress (strong light, dehydration and temperature shock) we applied Absolute real-time PCR technology to determine the transcription numbers of the selected test ...
关键词:constitutive expression gene housekeeping gene Porphyra yezoensis Ueda real-timequantitative PCR stress responding 
Characterization of small RNAs from Ulva prolifera by high-throughput sequencing and bioinformatics analysis被引量:4
《Chinese Science Bulletin》2011年第27期2916-2921,共6页HUANG AiYou WANG GuangCe HE LinWen NIU JianFeng ZHANG BaoYu 
supported by the Scientific and Technical Supporting Programs of China (2008BAC49B01);the National Natural Science Foundation of China (30830015)
The seaweed Ulva prolifera exists in 2 different states; attached to rocks or free-floating. However, there is little difference between the structures of the 2 states. U. prolifera thalli show significant differences...
关键词:小分子RNA 生物信息学 增殖 分析表征 石莼 测序 高通量 MIRNA 
Sequence and phylogenetic analyses of the chloroplast 16S rRNA, tufA, and rbcL genes from Bryopsis hypnoides被引量:1
《Chinese Journal of Oceanology and Limnology》2011年第5期922-929,共8页吕芳 王广策 
Supported by the National Natural Science Foundation of China (Nos. 30970302, 40806063, 30830015, B49082401)
Using shotgun sequencing data, the complete sequences of chloroplast 16S rRNA and tufA genes were acquired from native specimens of Bryopsis hypnoides (Qingdao, China). There are two group I introns in the 16S rRNA ge...
关键词:Bryopsis hypnoides BRYOPSIDALES biogeographic divergence phylogenetic analysis tufA gene 
浒苔小RNA高通量测序及相关生物信息学分析被引量:2
《科学通报》2011年第24期1993-1998,2041-2045,共6页黄爱优 王广策 何林文 牛建峰 张宝玉 
国家科技支撑计划(2008BAC49B01);国家自然科学基金(30830015)资助项目
浒苔有定生和漂浮两种状态,两者的形态结构没有显著差别,生长速度却有很大差异,推测浒苔可能存在重要的增殖调控模式.microRNA是非常重要的转录后调控因子,已证明其普遍存在于真核生物,在生长发育等过程中起重要作用.miRNA在高等动植物...
关键词:浒苔 小RNA miRNA solexa测序 生物信息学 
条斑紫菜低覆盖度基因组草图分析被引量:4
《海洋科学》2011年第6期76-81,共6页牛建峰 高胜寒 骆迎峰 袁野 王广策 胡松年 
国家863计划项目(2007AA09Z406);国家自然科学基金项目(30830015);国家973计划前期专项(2011CB411908)
对条斑紫菜(Porphyra yezoensis)进行Solexa高通量测序,获得低覆盖度全基因组草图。该基因组草图大小约220 Mbp,GC质量分数53.08%;包含26 629个预测基因,其中16 409个基因具有内含子,平均每个基因含2.22个内含子;基因结构分析表明具有...
关键词:条斑紫菜(Porphyra yezoensis) Solexa高通量测序 代谢通路 
Photosynthetic responses of thalli and isolated protoplasts of Bryopsis hypnoides(Bryopsidales,Chlorophyta)during dehydration被引量:1
《Chinese Journal of Oceanology and Limnology》2011年第2期334-342,共9页吕芳 王广策 靳皓琛 
Supported by the National Natural Science Foundation of China(Nos. 30970302,40806063,30830015,B49082401)
Bryopsis kypnoides Lamouroux is a unique intertidal siphonous green alga whose extruded protoplasm can aggregate spontaneously in seawater to form numerous new cells that can develop into mature algal thalli. In this ...
关键词:Bryopsis hypnoides chlorophyll fluorescence DEHYDRATION PROTOPLASTS pulse amplifiedmodulation fluorescence system 
坛紫菜磷酸烯醇式丙酮酸羧化酶基因的克隆与序列分析被引量:2
《海洋科学》2011年第4期70-76,共7页张晓娟 王广策 何林文 陈昌生 
国家自然科学基金重点项目(30830015);海洋公益性行业科研专项项目(201105008-2;201105023-7)
采用同源克隆的方法和RACE技术,从坛紫菜cDNA中获得了磷酸烯醇式丙酮酸羧化酶(PEPC)的序列,该段序列含2538个核苷酸包含一个终止密码子TAG和191 bp的3′端非编码区(UTR),可编码846个氨基酸。该段序列中无跨膜结构和信号肽序列,推导的氨...
关键词:坛紫菜 PEPC基因 序列分析 系统进化分析 
Comparison of Chlorophyll and Photosynthesis Parameters of Floating and Attached Ulva prolifera被引量:21
《Journal of Integrative Plant Biology》2011年第1期25-34,共10页Apeng Lin Songdong Shen Guangce Wang Qianqian Yi Hongjin Qiao Jianfeng Niu Guanghua Pan 
supported by the National Natural Science Foundation of China (30830015,30970302 and 40806063);the National Key Technologies R&D Program (2008BAC49B01);the Hi-Tech Research and Development Program of China (2006AA10A402 and 2007AA09Z406)
In mid-May 2008 a serious green tide caused mainly by floating Ulva prolifera (Mvller) J. Agardh (Chlorophyta, Ulvales) thalli struck the coastal area of Qingdao, China. To understand the present physiological con...
Optimization of conditions for tetraspore release and assessment of photosynthetic activities for different generation branches of Gracilaria lemaneiformis Bory被引量:19
《Chinese Journal of Oceanology and Limnology》2010年第4期738-748,共11页王志远 王广策 牛建峰 汪文俊 彭光 
Supported by the National Natural Science Foundation of China(No.30830015);the National Key Technology Research and Development Program of the 11th Five-Year Plan(No.2006BAD09A04);the National High Technology Research and Development Program of China(863Program,Nos.2006AA10A402,2007AA09Z406,2006AA05Z112,2006AA10A413)
Gracilaria lemaneiformis Bory is an economically important alga that is primarily used for agar production.Although tetraspores are ideal seeds for the cultivation of G.lemaneiformis,the most popular culture method is...
关键词:Gracilaria lemaneiformis Bory TETRASPORE pulse-amplitude-modulated PHOTOSYNTHESIS 
Analysis of expressed sequence tags from the Ulva prolifera (Chlorophyta)被引量:4
《Chinese Journal of Oceanology and Limnology》2010年第1期26-36,共11页牛建峰 胡海岩 胡松年 王广策 彭光 孙松 
Supported by the Scientific and Technical Supporting Programs of China (2008BAC49B01);the National Natural Science Foundation of China (No. 30830015)
In 2008, a green tide broke out before the sailing competition of the 29th Olympic Games in Qingdao. The causative species was determined to be Enteromorpha prolifera (Ulva prolifera O. F. Miiller), a familiar green...
关键词:green tide Ulva Prolifera expressed sequence tag (EST) filamentous green alga rapid growth PROLIFERATION 
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