This work proposed a label-flee, cost-effective and fairly sensitive electrogenerated chemiluminescence (ECL) strategy for the specific detection of lysozyme based on the hemin/G-quadruplex hybrid. Gold nanoparticle...
Project supported by the Natural Science Foundation of Shaanxi Province [No. 2001K 10-G3-(3)].
The refolding of reduced and non-reducing egg white lysozymes in a urea solution was studied by a "phase diagram" method of fluorescence. The result showed that in the refolding of the reduced egg white lysozyme, an...
Project supported by the National Natural Science Foundation of China (Nos. 20132020 and 20175026) and Henan Academic Foundation of Science and Technology.
Daidzein (7,4'-dihydroxyisoflavone) was phosphorylated by a modified Atherton-Todd reaction. The structures of the five target product, were determined by X-ray, IR, NMR and ESI-MS. Electrospray ionization results ...
Project supported by the Natural Science Foundation of Shaanxi Province [No. 2001K10-G3-(3)].
The aggregation interaction between reduced-denatured egg white lysozymes during refolding procedure in urea solution was studied by means of reducing and non-reducing protein electrophoreses. Results of non-reducing ...
Project supported by the National Natural Science Foundation of China (Nos. 39880003, 20175016).
Based on the monodisperse poly(glycidyl methacrylate-co-ethylenedimethacrylate) beads (PGMA/EDMA) with macropore as a medium, a new hydrophilic medium cation exchange (MCX) stationary phase for HPLC was synthesi...
Project supported by the Natural Science Foundation of Shaanxi Province (No. 2001K10-G3-3).
Based on three-state renaturation process of denatured proteins, an equation describing the effect of denaturant concentration on renaturation yield of denatured proteins was presented. By this equation, two parameter...