PRONUCLEUS

作品数：9被引量：27H指数：2
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相关领域：医药卫生更多>>
相关作者：高欣朱嘉骏宋敏国袁进强杨仙玉更多>>
相关机构：浙江农林大学中国科学院更多>>
相关期刊：《Zoological Research》《Journal of Animal Science and Biotechnology》《Developmental and Reproductive Biology》《Chinese Medical Journal》更多>>
相关基金：国家自然科学基金国家重点基础研究发展计划浙江省教育厅科研计划教育部留学回国人员科研启动基金更多>>
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Generation of mitochondrial replacement monkeys by female pronucleus transfer被引量：1
《Zoological Research》2024年第2期292-298,共7页Chun-Yang Li Xing-Chen Liu Yu-Zhuo Li Yan Wang Yan-Hong Nie Yu-Ting Xu Xiao-Tong Zhang Yong Lu Qiang Sun 
supported by the National Natural Science Foundation of China (82021001,31825018);National Key Research and Development Program of China (2022YFF0710901);Shanghai Municipal Science and Technology Major Project (2018SHZDZX05);Strategic Priority Research Program of the Chinese Academy of Sciences (XDB32060100);Biological Resources Program of Chinese Academy of Sciences (KFJ-BRP-005);National Science and Technology Innovation 2030 Major Program 2021ZD0200900。
Mutations in mitochondrial DNA(mtDNA)are maternally inherited and have the potential to cause severe disorders.Mitochondrial replacement therapies,including spindle,polar body,and pronuclear transfers,are promising st...
关键词：Non-human primates Mitochondrial replacement Female pronuclear transfer 
Syngamy,pronucleus,pronuclear breakdown and zygote
《Asian pacific Journal of Reproduction》2021年第5期239-240,共2页Mário Sousa Jan Tesarik 
It is partially supported by Unit for Multidisciplinary Research in Biomedicine(UMIB),funded by the Foundation for Science and Technology(FCT)Portugal(grant numbers UIDB/00215/2020,and UIDP/00215/2020);ITR-Laboratory for Integrative and Translational Research in Population Health(LA/P/0064/2020)(to Prof.Sousa).
The terms syngamy,pronucleus,pronuclear breakdown and zygote are common for experts dealing with reproductive cell morphology.However,these terms are often not correctly used in scientific and didactic literature.At f...
关键词：MORPHOLOGY BREAKDOWN dealing 
Pronuclear formation by ICSI using chemically activated ovine oocytes and zona pellucida bound sperm
《Journal of Animal Science and Biotechnology》2017年第2期370-377,共8页J.E.Hernández-Pichardo Y.Ducolomb S.Romo M.E.Kjelland R.Fierro F.Casillas M.Betancourt 
Background： In order to improve ICSI, appropiate sperm selection and oocyte activation is necessary. The objective of the present study was to determine the efficiency of fertilization using ICSI with chemically acti...
关键词：Ethanol ICSI Oocyte activation Ovine Pronucleus Zona pellucida 
Generation of human haploid embryonic stem cells from parthenogenetic embryos obtained by microsurgical removal of male pronucleus被引量：17
《Cell Research》2016年第6期743-746,共4页Cuiqing Zhong Meiling Zhang Qi Yin Han Zhao Yang Wang Sexin Huang Wenrong Tao Keliang Wu Zi-Jiang Chen Jinsong Li 
Acknowledgments We thank W Bian, X Wang and F Zhang from Cell Biology Facility for support with cell sorting, Y Chen and X Ding from the Stem Cell Core Facility at SIBCB for support with cell cultures, H Li, M Li, H Deng, R Hou and H Xiao from Shanghai Biochip Company for sequencing data analysis, and Y Jin and Y Zeng from SIBS for providing antibodies. This study was supported by the Ministry of Science and Technology of China （2014CB964803, 2012CB944700, 2015AA020307 and 2013CB967103） and the National Natural Science Foundation of China （91319310, 31225017, 31530048, 81430029, 81490743, 31371453 and 31571548）.
Haploid embryonic stem cells （haESCs） have been recently generated from parthenogenetic （PG） or andro- genetic （AG） blastoeysts of different mammals, including mouse, rat and monkey, enabling genetic screening a...
关键词：期刊 编辑工作 规范 写作方法 
DNA Methylation Pattern in Pronuclear-Stage Mouse Embryos:Effect of Oocyte Vitrification
《Journal of Animal Science and Biotechnology》2011年第4期192-198,共7页Ying Liang Xiangwei Fu Junjie Li Dianshuai Yuan Shien Zhu 
This study was conducted to investi- gate the pattern of DNA methylation in pronuclearstage mouse embryos derived from vitrified-warmed oocytes. Mouse oocytes at metaphase II （MII） stage of meiosis were allocated ra...
关键词：DNA methylation mouse OOCYTE open-pulled straw vitrification PRONUCLEUS 
Localization of stationary pronuclei during conjugation of Paramecium as indicated by immunofluorescence staining被引量：2
《Zoological Research》2011年第4期461-464,共4页高欣 朱嘉骏 杨仙玉 袁进强 王逸雯 宋敏国 
国家自然基金项目(30670397;31071181);教育部留学回国人员科研启动基金项目([2007]1108);浙江省教育厅一般项目(Y200804256);浙江农林大学科研发展基金项目(2292000030)
After the third prezygotic division during conjugation of Paramecium caudatum,migratory and stationary pronuclei are produced.The migratory pronuclei remain in the paroral region tightly against the conjugating bounda...
关键词：PARAMECIUM CONJUGATION Connecting spindles PRONUCLEUS 
Comparative studies on in vitro sperm decondensation and pronucleus formation in egg extracts between gynogenetic and bisexual fish被引量：7
《Cell Research》2003年第3期159-169,共11页CHANG JIAN LI,JIAN FANG GUIState. Key Laboratory of Freshwater Ecology and Biotechnology,Wuhan Center for Developmental Biology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China 
supported by grants from the National Natural Science Foundation of China(No.30130240 and 30070379);the Project of Chinese Academy of Sciences(KSCX2-SW-303);the Frontier Science Projects Program of the Institute of Hydrobiology,Chinese Academy of Sciences(No.220309).
A cell-free system based upon the egg extracts from gynogenetic gibel carp (Carassius auratus gibelio)or bisexual red common carp (Cyprinus carpio red variety) was developed to investigate developmentalbehaviors of th...
关键词：decondensation pronucleus formation cell-free system GYNOGENESIS gibel carp. 
Studies on renin-2 gene in transgenic rats
《Chinese Medical Journal》1999年第5期20-25,共6页陈兰英 方福德 田小利 刘红 赵淑华 
Objective To determine the function of, in vivo, renin and its role in the pathogenesis of hypertension. Methods A renin 2 gene restriction map was constructed by endonuclease digest ion and Southern blotting hy...
关键词：mouse renin 2 gene · transgenic rat · pronucleus microinjection · hypertension · restriction map 
Chronological and Morphological Progression of Nucleus during Mouse Oocyte Maturation and Fertilization in vitro
《Developmental and Reproductive Biology》1996年第1期24-33,T001-T004,共14页孙青原 刘灵 李明文 段崇文 刘辉 陈大元 
The chronological and morphological changes of the nucleus during mouse oocyte maturation and fertilization were systematically studied. Although most oocytes went through GVBD 2-4 hrs after culture, 13.6% remained at...
关键词：mouse germinal vesicle breakdown (GVBD) pronucleus formation polar body (PB) NUCLEOLUS 
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