HYBRIDOMAS

作品数：6被引量：13H指数：2
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相关领域：医药卫生农业科学更多>>
相关期刊：《Academic Journal of Xi'an Jiaotong University》《World Journal of Gastroenterology》《Advances in Bioscience and Biotechnology》《Cell Research》更多>>
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Monoclonal antibody technology applied to the detection of <i>Cryptosporidium parvum</i>oocysts in human and cattle fecal samples
《Advances in Bioscience and Biotechnology》2013年第4期7-16,共10页Vera Codices Olga Matos Carlos Novo 
With the discovery that the coccidian parasite Cryptosporidium sp. can cause severe symptoms in humans, many diagnostic techniques were quickly implemented such as microscopic visualization, immunofluorescence and PCR...
关键词：HYBRIDOMAS Cryptosporidium PARVUM Immunofluorescence DETECTION 
Downregulation of NFAT5 by RNA interference reduces monoclonal antibody productivity of hybridoma cells被引量：4
《Cell Research》2007年第3期264-270,共7页Jihang Ju Ke Zou Hong Xie 
Hybridoma cells display an increase in antibody productivity following exposure to hypertonic conditions. However, the underlying mechanism is not well understood. In the present study, we hypothesize that the nuclear...
关键词：antibody formation HYBRIDOMAS NFAT transcription factors RNA interference 
THE EUKARYOTIC EXPRESSION OF HUMAN PERFORIN PROTEIN AND THE ESTABLISHMENT OF HYBRIDOMAS PRODUCING ANTI-HUMAN PERFORIN PROTEIN
《Academic Journal of Xi'an Jiaotong University》2001年第1期77-80,共4页刘培军 王一理 耿宜萍 阎晓彩 司履生 
This research was supported by Natural Science Foundation of China(No. 39200045).
Objective To prepare the monoclonal antibody against human perforin(HP). Methods Recombi- nant eukaryotic expression plasmid pCDM8-HP was extracted and purified, and the BALB/C mice were immunized with the plasmid. Th...
关键词：naked gene immunization human perforin(HP) monoclonal antibody(McAb) 
Preparation of single chain variable fragment of MG_7 mAb by phage display technology被引量：9
《World Journal of Gastroenterology》2001年第4期510-514,共5页Zhao-Cai Yu Jie Ding Yong-Zhan Nie Dai-Ming Fan Xue-Yong Zhang Department of Gastroenterology,Xijing Hospital,Fourth Military Medical University,Xi’an 710032,Shaanxi Province,China 
AIM: To develop the single chain variable fragment of MG MG(7)murine anti-human gastric cancer monoclonal antibody using the phage display technology for obtaining a tumor-targeting mediator. METHODS: mRNA was isolate...
关键词：Peptide Library Animals Antibodies  Monoclonal Antibody Specificity Cell Line Enzyme-Linked Immunosorbent Assay Gene Therapy Genetic Screening Humans HYBRIDOMAS Immunoglobulin Variable Region Mice Recombination  Genetic Stomach Neoplasms 
Relationship between chromosome behaviour and stability of human-mouse and human-(human-mouse)hvbridomas
《Journal of Medical Colleges of PLA(China)》1993年第2期114-119,共6页梁喆 王德堂 金伯泉 
Chromosomes in human-mouse and human-(human-mouse)hybridomas wereanalysed by G-banding methods.It was found that most human chromosomes,exceptNo.13 and X,Y,were retained.The frequencies of chromosomes No.1,3,4,5,6,17,...
关键词：HYBRIDOMAS IMMUNOGLOBULINS GIEMSA STAINING chromosomes human 1-3 chromosomes HUMAN 13-15 chomosomes human 21-22 
Some biological characteristics of hybridomas and parental myelomas cultivated in serum-free medium for long-term period
《Journal of Medical Colleges of PLA(China)》1992年第3期246-250,共5页李纪良 李英杰 巢穗 欧阳明辉 彭一兵 
Partially supported by grants from the National Natural Science Foundation of China(No.3880715);General Logistics Department of PLA;and the President Foundation of the First Military Medical University
It has been developed in this laboratory that a serum-free medium, designated asDMI,is adaptive for long-term culture,freezing and resurgence in liquid nitrogen,and cloningof hybridoma and parental myelomas as well as...
关键词：MYELOMA HYBRIDOMA SERUM-FREE medium MONOCLONAL antibody 
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