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Surveillance of High-Risk Type-16 and 18 Human Papillomavirus among HIV Positive and Non-Positive Individuals at Federal Teaching Hospital Ebonyi State Nigeria
《Advances in Microbiology》2024年第12期647-662,共16页Euslar Nnenna Onu Donatus Chukwuma Ilang Cornelius Ituma Azi Theophilus Onwe Eze Chinedu Obasi Akpa Okechukwu Emmanuel Ogbonna Samuel Eleojo Agada Michael Chinedu Onwe 
This study was carried out to determine the seroprevalence of high risk human papillomavirus type-16 and type-18 among people living with HIV and individuals who are seronegative to HIV infection. A total of 400 Blood...
关键词：Human Papillomavirus Mortality ANTIBODIES PRIMERS CERVIX Cancer 
A single degenerated primer significantly improves COX1 barcoding performance in soil nematode community profiling
《Soil Ecology Letters》2024年第2期31-44,共14页Yincai Ren Dorota L. Porazinska Quanhui Ma Shuhan Liu Hongmei Li Xue Qing 
supported by the National Natural Science Foundation of China(Grant number 32001876).
A new COX1 primer for soil nematode metabarcoding was designed,and this primer outperforms other commonly used COX1 primer pairs in species recovery and quantity of PCR products.•The lack of reference database is the ...
关键词：degenerated primers DNA metabarcoding mitochondrial cytochrome oxidase c subunit I gene PHYLOGENY ribosomal RNA gene soil nematodes 
Translating Pathological Brain Activity Primers in Parkinson’s Disease Research
《Research》2024年第1期735-752,共18页Daniela Mirzac Svenja L.Kreis Heiko J.Luhmann Gabriel Gonzalez-Escamilla Sergiu Groppa 
the German Research Foundation(DFG;SFB-TR-128).
Translational experimental approaches that help us better trace Parkinson’s disease(PD)pathophysiological mechanisms leading to new therapeutic targets are urgently needed.In this article,we review recent experimenta...
关键词：CLINICAL knowledge INSIGHT 
Simultaneous analysis of the intestinal parasites and diet through eDNA metabarcoding被引量：2
《Integrative Zoology》2023年第3期399-413,共15页Xabier CABODEVILLA Benjamín Juan GÓMEZ-MOLINER Naiara ABAD María JoséMADEIRA 
Xabier Cabodevilla was supported by a PhD grant,financed by the Basque Country Government(Grants no.PRE_2018_2_0273);a contribution to project Sistemática,Biogeografía,Ecología del comportamiento y Evolución(IT1163-19);funded by Basque Country Government;Additional funds for this study were provided by the project 201630E096 funded by CSIC.
Agricultural expansion and intensification are having a huge impact on plant and arthropod diversity and abun-dance,affecting food availability for farmland birds.Difficult food access,in turn,can lead to immunosuppress...
关键词：BIOMONITORING broad-spectrum primers eukaryotic mini-barcodes fecal eDNA farmland birds PARASITOLOGY 
Single-stranded RNA as primers of terminal deoxynucleotidyl transferase for template-independent DNA polymerization
《Chinese Chemical Letters》2023年第2期161-164,共4页Houyu Han Jingyu Cui Dianming Zhou Deping Hua Weipan Peng Mengyao Lin Yating Zhang Fangya Li Xiaoqun Gong Jianyu Zhang 
supported by the National Natural Science Foundation of China (NSFC, Nos. 21927814 and 21772143 to J.Y. Zhang);the National Science Foundation of Tianjin (Nos. 20YDTPJC00090, 19ZXDBSY00070 and 20YFZCSY00990 to X.Q. Gong)。
Terminal deoxynucleotidyl transferase(Td T) has been characterized as template-independent polymerase using single-stranded DNA(ss DNA) as primers to generate random oligonucleotides. However, the extension performanc...
关键词：Terminal deoxynucleotidyl transferase Single-stranded RNA Single-stranded DNA Template-independent POLYMERIZATION 
Comparison of 16S rRNA Gene Primers on Studying Microbial Community Composition in Bottom Water and Sediment of Artificial Reefs in Laoshan Bay, China
《Journal of Ocean University of China》2022年第5期1313-1322,共10页FANG Guangjie YU Haolin SHENG Huaxiang TANG Yanli LIANG Zhenlin 
funded by the Project of National Key R&D Program of China(No.2019YFD0901302).
Marine microorganisms are indispensable regulators of nutrient cycling and energy flow,which are crucial for artificial reefs(ARs)ecosystems.However,little is known about the microbial communities in the bottom water ...
关键词：16S rRNA hypervariable region ARs bacteria ARCHAEA 
Evaluation of different primers of the 18S rRNA gene to profile amoeba communities in environmental samples被引量：1
《Water Biology and Security》2022年第3期56-66,共11页Xiafei Zheng Zhili He Cheng Wang Qingyun Yan Longfei Shu 
supported by the National Natural Science Foundation of China(31970384,41907021,21806044,92051120,31802350);the Fundamental Research Funds for the Central Universities Sun Yat-sen University(22lgqb22,19lgzd28);the Innovation Group Project of Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai)(311021006);the Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai)(SML2021SP203);the Guangdong Natural Resources Department Contract(GDNRC[2021]62);Guangzhou Basic and Applied Basic Research Foundation(202102020257)。
Amoeboid protists,an assemblage of organisms belonging to different phylogenetic lineages,have drawn increasing attention due to their crucial ecological roles in various environments and their potential health risks....
关键词：Amoeba community Diversity Drinking water safety METAGENOMICS 18S rRNA gene PROTIST 
Methodology for fish biodiversity monitoring with environmental DNA metabarcoding:The primers,databases and bioinformatic pipelines被引量：10
《Water Biology and Security》2022年第1期63-72,共10页Fan Xiong Lu Shu Honghui Zeng Xiaoni Gan Shunping He Zuogang Peng 
supported by the grant from the Graduate Student Research Innovation Project of Chongqing(CYB20093)。
Environmental DNA(eDNA)originates from cellular material shed by organisms into aquatic or terrestrial environments and can be sampled and monitored using metabarcoding technology,which is revolutionizing fish biodive...
关键词：Environmental DNA Fish biodiversity Metabarcoding METHODOLOGY 
Identification and evaluation of enzymatic ability of fungal endophytes from Citrofortunella microcarpa(Bunge)Wijnands
《Studies in Fungi》2021年第1期460-468,共9页Reyes RDC Parayao AM Waing KGD 
Citrofortunella microcarpa locally known as“kalamansi”belongs to the family Rutaceae is one of the most important marketable fruit crops grown in the Philippines.Aside from being a fruit crop,the plant also houses v...
关键词：ENZYMES EXTRACELLULAR ITS primers PLEOSPORALES 
An enhanced method for nucleic acid detection with CRISPR-Cas12a using phosphorothioate modified primers and optimized gold-nanopaticle strip被引量：1
《Bioactive Materials》2021年第12期4580-4590,共11页Jiaojiao Gong Lijuan Kan Xiuming Zhang Ying He Jiaqiang Pan Liping Zhao Qianyun Li Menghao Liu Jie Tian Sili Lin Zhouyu Lu Liang Xue Chaojun Wang Guanghui Tang 
This work was supported by the Discipline Construction Ability Promotion Project of Shenzhen Health and Population Family Planning Commission(NO.SZXJ2018031(Kan L.J.));Sanming Project of Medicine in Shenzhen(NO.SZSM201601062(Zhang X.M.));Shenzhen Key Medical Discipline Construction Fund(NO.SZXK054(Zhang X.M.)).
CRISPR-Cas12a system has been shown promising for nucleic acid diagnostics due to its rapid,portable and accurate features.However,cleavage of the amplicons and primers by the cis-and trans-activity of Cas12a hinders ...
关键词：CRISPR-Cas12a Onepot Visual detection 
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