Supported by the National Key R&D Program of China(No.2018YFC1406406);the Science and Technology Program of Yantai(Nos.2018SFBF084,2019ZDCX018)。
Fish embryos are widely used as models in toxicology,drug development,and human disease research because of their high sensitivity,observability,and operability,providing the basis for an in-depth understanding of the...
supported by the National Natural Science Foundation of China (Nos. 31621004 and 81571356);the Key Research Projects of the Frontier Science of the Chinese Academy of Sciences (QYZDY-SSWSMC002);the Key Deployment Projects of the Chinese Academy of Sciences (ZDRW-ZS-2017-5);the Youth Innovation Promotion Association CAS (No. 2017113)
Following fertilization in mammals, the zygote initiates the developmental program, which has a transient capacity to generate cell types of both embryonic and extraembryonic lineages, which is defined as totipotency ...
We thank Dr Xiang Gao of Nanjing University for providing EGFP-transgenic mice. We thank Drs Yixian Zheng of Carneige Institutation and Haifan Lin of Yale University for their critical and useful comments on the manuscript. This work was supported by grants from the Chinese Academy of Sciences (KSCX2-YW-R- 110, KSCX2-YW-R-229, XDA01010403), the Ministry of Science and Technology (2007CB947101, 2009CB941101), National Natural Science Foundation of China (30871430) and the Shanghai Municipal Commission for Science and Technology (07DZ22919, 08DJ1400502, 09PJ1410900).
Dear Editor, It is well known that mature oocytes can reprogram somatic nuclei in the process of nuclear transfer (NT) [1] and remodel male gametes in normal fertilization. An intriguing question is whether blastom...
Mouse cloning from fertilized eggs can assist development of approaches for the production of "genetically tailored" human embryonic stem (ES) cell lines that are not constrained by the limitations of oocyte avail...
supported by Grants (20060102A1022) from State 863 projects;Key projects of Science and Technology Commission of Shanghai (09ZR1419000)
Objective To investigate whether isolated blastomeres of discarded human embryos could develop into blastocysts cultured in vitro without zona pellucida. Methods Total discarded 60 embryos, which were not suitable for...
Timing of vegetal-endodermal cell determination in amphioxus embryos remains uncertain. We tentatively testal effects of A23187, the calcium ionophore, on the deveopment of vegetal blastomeres isolated at the 16-cell ...
The derso-ventral axis of Xenopus embryo is established after fertilization. Blastula stage blastomeres acquire different identities as they have inherited different maternal materials which distribute radial symmetri...
Since the first report on the successful deep cryopreservation of mam-malian embryos in 1972,slow progressing cooling rate has been employ-ed in conventional embryos-freezing techniques;while more recent studieson fre...