PROTEINASE

作品数:55被引量:134H指数:6
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相关领域:医药卫生更多>>
相关作者:杨英军周鹏余长缨石铁流蒋华良更多>>
相关机构:中国热带农业科学院南京大学中国科学院上海生命科学研究院河南科技大学更多>>
相关期刊:《Advances in Microbiology》《Agricultural Sciences》《Progress in Natural Science:Materials International》《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》更多>>
相关基金:国家自然科学基金国家重点基础研究发展计划国家高技术研究发展计划北京市自然科学基金更多>>
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Isolation of tomato proteinase inhibitor Ⅱ gene and the function of its intron被引量:2
《Chinese Science Bulletin》2002年第10期830-834,共5页Xie, XZ Wu, NH 
This work was supported by the Langqidao Company, Fuzhou Municipality, Fujian Province; and the State Tobacco Monopoly Administration.
The genomic DNA sequence of tomato pro-teinase inhibitor II gene (named tin2i, whose accession number in GenBank is AF007240) was isolated by PCR techniques. The intron sequence (TPI), with a length of 109 bp, owns ty...
关键词:TOMATO PROTEINASE INHIBITOR II GENE INTRON enhancer. 
Expression of matrix metallo-proteinase-28 in human normal cytotrophoblast cells and a choriocarcinoma cell line,JEG-3被引量:4
《Chinese Science Bulletin》2002年第9期732-736,共5页Zhang, J Cao, YJ Liu, WM Zhao, BS Zeng, GQ Duan, EK 
This work was supported by the Special Funds for the Major State Basic Research Project (Grant No. G1999055903);the Climbing Project of China (Grant No. 970211019-3);the Hundred-Scientist-Program of the Chinese Academy of Sciences.
There are striking similiarities present between the behavior of invasive placentaL cells and that of invasive cancer cells. Matrix metalloproteinases (MMPs) are one of the most important mediators. MMP-28, the new me...
关键词:CYTOTROPHOBLAST CELL CHORIOCARCINOMA CELL line JEG-3 MATRIX metalloproteinases MMP-28. 
Differential expression analysis of matrix metallo-proteinase 20(MMP20) in human esophageal cancer
《Chinese Science Bulletin》2001年第12期1009-1012,1057,共5页YANG Cuihong LIU Zhihua WANG Qun LUO Aiping SUN Yuntian ZHANG Xun WANG Xiuqin ZHOU Chuannong WU Min 
This work was supported by the State Key Basic Research Program (Grant Nos. G1998051021 and G1998051205); and the National Natural Science Foundation of China (Grant Nos. 39993420 and 39990570).
The aim of this study was to investigate the different expressions of MMP20 in the process of esophageal cancer. RT-PCR, immunohistochemistry and Western blot were employed to detect the mRNA expression and protein ex...
关键词:ESOPHAGEAL cancer MMP20 RT-PCR WESTERN blot. 
A new method of research on molecular evolution of proteinase superfamily被引量:1
《Chinese Science Bulletin》2001年第7期578-582,共5页SUN Zhirong JI Xinglai HU Shengmin WANG Yu HE Fuchu 
in part by the NationalNatural Science Foundation of China (Grant Nos. 39730310 and 39980007).
The molecular evolutionary tree, also known as a phylogenetic tree, of the serine proteinase superfamily was constructed by means of structural alignment. Three-dimensional structures of proteins were aligned by the S...
关键词:molecular evolution SSAP algorithm structure alignment EVOLUTIONARY tree SERINE PROTEINASE superfamily. 
NMR studies of the interaction between Bacillis subtilis neutral proteinase and inorganic metal compounds
《Chinese Science Bulletin》2000年第19期1764-1766,共3页ZHENG Xuefang HU Jiehan XU Yongting CHENG Guobao GAI Hongwei 
The interaction between Bacillis subtilis neutral proteinase (B.S.NP) and inorganic metal compounds (CoCI2, NiCI2) was investigated by 1H NMR spectroscopy. It has been shown that the Zn(Ⅱ) ion in the active center of...
关键词:Bacillis SUBTILIS NEUTRAL PROTEINASE CoCl2 NiCl2 interaction NMR. 
33 ku protein associated several polypeptides with nearly the same molecular weight but not the same isoelectric point被引量:3
《Chinese Science Bulletin》2000年第1期57-60,共4页Hui Yu Bin Kang Jie Wei Liangbi Li Tingyun Kuang 
The 33 ku protein, prepared from NaCI-treated PSII particles, has shown an single band by SDS-PAGE. After being dialyzed against the low-osmotic medium at 4癈, it has been found that the 33 ku protein degraded into se...
关键词:33 KU protein PROTEINASE isoelectric point isoelectric FOCUSING 2-D (2-dimensional) electrophoresis. 
Transgenic Tobacco Plants by Cotransformation With Proteinase Inhibitor Ⅱ and δ-endotoxin Genes
《Chinese Science Bulletin》1993年第18期1561-1566,共6页华学军 陈晓邦 马万超 范云六 
In recent years, great progress has been made in. producing plants resistant to insect attack by genetic engineering of B. t δ-endotoxin gene or plant proteinase inhibitor genes, and how to avoid the emergence of the...
关键词:PROTEINASE inhibitor GENE B. t δ-endotoxin GENE COTRANSFORMATION TRANSGENIC plants GENE expression. 
CRYSTALLOGRAPHIC STUDY OF ORTHOR-HOMBIC CRYSTALS OF PROTEINASE INHIBITOR FROM ARROWHEAD (agittaria sagittifolia, Linn)
《Chinese Science Bulletin》1988年第21期1816-1818,共3页林光大 张荣光 唐伟中 王丽秀 梁栋材 戚正武 曹天钦 
Ⅰ. INTRODUCTION Proteinases and their inhibitors are widely distributed in nature. They play important roles in the biological regulation and control of a number of physiological processes. More than one hundred inhi...
关键词:erystallization PROTEINASE INHIBITOR 
THE CRYSTALLIZATIONS OF FRACTION I PROTEIN IN LEAVES OF Brassica napus AND Nicotina tobacum
《Chinese Science Bulletin》1987年第7期482-483,共2页应燕如 蔡以欣 孙崇荣 
Fraction I Protein (F-I-P) was first found in spinach mesophyll cells by Wildman and Bonner in 1947. Then, it was demonstrated that F-I-P existed wildly in plant kingdom and was a major component of soluble proteins i...
关键词:tobacco CARBOXYLASE crystallize OXYGENASE calls NICOTIANA SUPERNATANT PROTEINASE wheat fresh 
THE CRYSTALLIZATION AND PRELIMINARY CRYSTALLOGRAPHIC STUDY OF SEVERAL COMPLEX CRYSTALS BETWEEN PORCINE CHYMOTRYPSIN AND TRYPSIN
《Chinese Science Bulletin》1987年第4期247-251,共5页曾杰 陈忠国 李根培 唐有祺 魏新成 卢光莹 顾孝诚 戚正武 
The 3-dimensional structures of bovine chymotrypsin and bovine trypsin are one of the earliest fruitful achievements in the field of protein crystallography. In 1970, R. M. Stroud et al. disclosed the stereoscopic str...
关键词:TRYPSIN BOVINE fruitful earliest PROTEINASE extinction saturation PRELIMINARY HANGING seldom 
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