FMDV

作品数：275被引量：630H指数：11
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  • 期刊=Agricultural Science & Technologyx
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Construction of DNA Vaccine for FMDV P1 Gene and Immunization Experiment
《Agricultural Science & Technology》2013年第8期1069-1071,共3页史秋梅 高桂生 张艳英 高光平 张东林 
科技部农业成果转化项目(2012GB2A200045);农业部转基因专项(2011ZX08006-001);2012年河北科技师范学院动物疫病防控科研创新团队项目~~
[Objective] This study aimed to construct DNA vaccine of foot-and-mouth disease （FMD）.[Method] Plasmid carriers plESZP1 and pUTK3CP1 with PRV were constructed for FMDV P1 gene expression.Mice were immunized,and thei...
关键词：FMDV P1 gene DNA plasmid Immunization experiment 
Expression of Recombinant Gene P1-2A3C of FMDV Asia I in Different Bombyx mori Varieties被引量：1
《Agricultural Science & Technology》2011年第10期1528-1530,共3页张韵 张金卫 易咏竹 李志勇 李江涛 鱼南洋 丁农 柳纪省 
Supported by863Project for Development and Production of New FMDV accine for Livestock(2011AA10A211);Fund for Agricultural Since and Technology Achievements Transformation(2009GB23260437)~~
[Objective] The aim was to investigate the expression level of recombinant gene P1-2A3C of FMDV Asia I in different Bombyx mori varieties,so as to screen out the B.mori varieties suitable for the foreign gene expressi...
关键词：Asia I FMDV P1-2A3C Bombyx mori varieties 
Prokaryotic Expression of P1 Gene of Type Asia1 Foot and Mouth Disease Virus(FMDV)and the Preparation of Its Antiserum
《Agricultural Science & Technology》2010年第9期112-114,143,共4页武刚 王洪梅 刘晓 王立群 于力 仲跻峰 何洪彬 
Supported by National Transgenic Major Program of China(2009ZX08007-006B);the National Natural Science Foundation of China(31072160);Science and Technique Foundation of Shandong Province(2009GG20002032);Natural Science Foundation ofShandong Province(Y2008D20);an Open Issue of State Key Laboratory of Veterinary Biotechnology Fund(SKLVBF200806)~~
[Objective] The aim was to study the prokaryotic expression of P1 gene of foot-and-mouth disease virus（FMDV）type Asia 1and the preparation of its antiserum.[Method]The P1 gene of FMDV type Asia 1 was obtained by gen...
关键词：Foot-and-mouth disease virus（FMDV） P1 gene Prokaryotic expression ANTISERUM 
Expression,Purification and Activity Detection of VP1 of A-type FMDV被引量：4
《Agricultural Science & Technology》2010年第2期23-26,共4页李菁 林彤 高闪电 丛国正 独军政 邵军军 常惠芸 
Supported by National Supporting Plan(2006BAD06A14);Transgenic Major Projects(2008ZX08011-004)~~
[Objective] The research aimed to induce the expression of FMDV structural protein VP1 in E.coli and purify the protein,then detect the activity.[Method] The fragment coding VP1 was amplified by PCR and doubly digeste...
关键词：A-type FMDV Structural protein VP1 Expression and purification 
Expression and Identification of the Type-specific Antigen of FMDV of Serotype C被引量：4
《Agricultural Science & Technology》2009年第5期79-81,95,共4页高闪电 独军政 常惠芸 丛国正 邵军军 林彤 宋帅 谢庆阁 
Supported by Key Projects in the National Science &Technology Pillar Program during the Eleventh Five-year Plan Period (2006BAD06A14)~~
[Objective]The aim was to provide a theoretical basis for preparation of polyclonal antibodies and monoclonal antibody that of type specificity, as well as Foot-and-mouth Disease Virus （FMDV） typing.[Method] The rec...
关键词：Foot-and-mouth disease virus of serotype C SEROLOGY Cross reactivity SEROTYPING 
Establishment of Monoclonal Antibody Competitive ELISA Using Monoclonal Antibody Against VP1 Protein of Asia 1 Type Foot-and-Mouth Disease Virus被引量：4
《Agricultural Science & Technology》2009年第3期104-107,共4页林彤 邵军军 丛国正 独军政 高闪电 常惠芸 谢庆阁 
Supported by National High-tech R&D Program (863) Subsidized Project(2006AA10A204);Special Fund for Basic Scientific Research-related Subsidy of State-level and Public-welfare Scientific Research Institutes~~
Using the purified VP1 protein of Asia 1 type foot-and-mouth disease virus as the antigen, the purified monoclonal antibody was labeled by the sodium periodate method and the monoclonal antibody competitive ELISA was ...
关键词：Asia 1 FMDV VP1 monoclonal antibody Competitive ELISA 
Cloning and Prokaryotic Expression of VP1 Gene of Foot-and-Mouth Disease Virus (FMDV) Type O被引量：2
《Agricultural Science & Technology》2008年第5期55-58,154,共5页付薇 陈磊 熊毅 潘琼 王常伟 陈进喜 胡晓静 刘棋 
Supported by the Science and Technology Foundation from Science&Technology Department of Guangxi Autonomous Region(0779001)~~
According to the complete genome of foot-and-mouth disease virus(FMDV)type O,a pair of special primers was designed to amplify VP1 gene.The VP1 gene was amplified by RT-PCR and subsequently inserted into the expressio...
关键词：Foot-and-mouth disease virus Structural protein VP1 CLONING Expression 
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