FUSION_PROTEIN

作品数：166被引量：380H指数：10
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相关作者：黄鹤孙汭田志刚王婉郑晓东更多>>
相关机构：军事医学科学院第三军医大学西南医院中国科学技术大学天津大学更多>>
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Efficient gene disruption in polyploid genome by Cas9–Trex2 fusion protein
《Journal of Integrative Plant Biology》2025年第1期7-10,共4页Wenbo Pan Chunlei Gao De Niu Jinghua Cheng Jiao Zhang Xiying Yan Qiang Long Yao Yao Zhu Wenjing Sun Qi Xie Yuehui He Xing Wang Deng Huawei Zhang Jian Li 
supported by the Natural Science Foundation of Shandong Province(ZR2021ZD30,ZR2022ZD22);the Key R&D Program of Shandong Province(ZR202211070163);Taishan Scholar Foundation of Shandong Province(TSQN202103160);the Excellent Youth Foundation of Shandong Scientific Committee(ZR202103010168)。
Despite significant advancements in the efficiency of the CRISPR/Cas9 genome editing system over the past decade,performing gene disruption in polyploid crops such as common wheat(Triticum aestivum L.)remains challeng...
关键词：CRISPR/Cas9 CROPS TRAIT 
Establishment of High-sensitivity Rapid Fluorescence Quantitative Detection Method for Antibody against Peste des Petits Ruminants Virus
《Agricultural Biotechnology》2024年第5期22-27,共6页Zhao LIU Bo LIU Zhida LIN Hang SUN Yu SUN Xiaohui SONG 
Supported by The National Project for the Prevention and Control of Major Exotic Animal Diseases(2022YFD1800500);National Mutton Sheep Industrial Technology System(CARS39).
[Objectives]This study was conducted to establish a rapid quantitative method for detecting antibody against Peste des Petits Ruminants Virus(PPR V)in sheep serum.[Methods]Soluble N protein and NH fusion protein were ...
关键词：Peste des Petits Ruminants N protein NH fusion protein Soluble expression and purification Rapid quantitative detection 
Establishment of Double-antigen Sandwich Time-resolved Fluorescence Immunoassay for Detection of Pest des Petits Ruminants Virus
《Agricultural Biotechnology》2024年第4期21-27,共7页Binglei CAO Zhongyuan GE Qi YANG Hang SUN Yu SUN Xiaohui SONG 
Supported by National Key R&D Program for the Prevention and Control of Major Exotic Animal Diseases(2022YFD1800500);National Mutton Sheep Industrial Technology System(CARS39);Key Research and Development Program of Shandong Province(Major Science and Technology Innovation Project)(2021CXGC011306);Scientific Research Project of General Administration of Customs(2024HK033);Scientific Research Project of Jinan Customs(2023JK005).
[Objectives]This study was conducted to explore rapid and large-scale screening and detection of peste des petits ruminants(PPR),so as to provide important technical means for prevention,control and purification of PP...
关键词：Peste des petits ruminants N active protein NH fusion protein Soluble expression and purification Time-resolved fluorescence immunoassay 
Aszonapyrone A Isolated from Neosartorya spinosa IFM 47025 Inhibits the NF-κB Signaling Pathway Activated by Expression of the Ependymoma-Causing Fusion Protein ZFTA-RELA
《Advances in Microbiology》2024年第9期448-467,共20页Kazuki Ishikawa Nao Kamiya Masaki Ishii Takashi Yaguchi Koji Ichinose Shinya Ohata 
Ependymoma is a rare and chemotherapy-resistant brain tumor, which has resulted in a delay in the development of drugs to treat it. A subclass of supratentorial ependymomas (ST-EPN), designated ST-EPN-zinc finger-tran...
关键词：Aszonapyrone A Neosartorya spinosa NF-κB Signaling Pathway EPENDYMOMA ZFTA-RELA 
Hyperthermia promotes degradation of the acute promyelocytic leukemia driver oncoprotein ZBTB16/RARα被引量：1
《Acta Pharmacologica Sinica》2023年第4期822-831,共10页Qian-qian Wang Liaqat Hussain Pei-han Yu Chang Yang Chen-ying Zhu Ya-fang Ma Si-chun Wang Tao Yang Yuan-yuan Kang Wen-juan Yu Yasen Maimaitiyiming Hua Naranmandura 
supported by grants from the National Natural Science Foundation of China(No.81872942,82003875,82000155,82000145,82200160);the China Postdoctoral Science Foundation(No.2021M702875,2021M702877);the Leading Innovative and Entrepreneur Team Introduction Program of Zhejiang,Grant/Award Number:2020R01006.
The acute promyelocytic leukemia(APL)driver ZBTB16/RARαis generated by the t(11;17)(q23;q21)chromosomal translocation,which is resistant to combined treatment of all-trans retinoic acid(ATRA)and arsenic trioxide(ATO)...
关键词：acute promyelocytic leukemia ZBTB16/RARαfusion protein HYPERTHERMIA PROTEOLYSIS nuclear receptor corepressors ARSENICALS 
Functionalization of micropipette tips with hydrophobin-laccase chimera and application to the electrochemical determination of caffeic acid in tea samples
《Food Bioscience》2023年第2期1362-1368,共7页Ilaria Stanzione Anna Pennacchio Alessandra Piscitelli Paola Giardina b Estefania Costa-Rama M.Teresa Fernandez-Abedul 
Performing on-site measurements is one of the main challenges of current(bio)chemical analysis.Micropipette tips,which are among the most common utilities in laboratories,can be useful and cheap elements for designing...
关键词：Fusion protein LACCASE Micropipette tips Electrochemical detection Caffeic acid 
A simple nanobody-based competitive ELISA to detect antibodies against African swine fever virus被引量：5
《Virologica Sinica》2022年第6期922-933,共12页Jiakai Zhao Jiahong Zhu Ying Wang Mengting Yang Qiang Zhang Chong Zhang Yuchen Nan En-Min Zhou Yani Sun Qin Zhao 
supported by the Natural Science Foundation of China (grant no. 31941016 and 31972676);the Natural Science Foundation of Shaanxi Province of China (grant no. 2022JC-12);the Key R&D Program of Shaanxi Province (grant no. S2022-YF-YBNY0673)
African swine fever virus(ASFV) infection is a big threat to the global pig industry. Because there is no effective vaccine, rapid, low-cost, and simple diagnosis methods are necessary to detect the ASFV infection in ...
关键词：African swine fever virus(ASFV) NANOBODY ASFV-p30 Nanobody-HRP fusion Protein Competitive ELISA 
Construction of Expression Vector for Porcine Gastrin-releasing Peptide Fusion Protein被引量：1
《Agricultural Biotechnology》2022年第3期72-74,共3页Zhiyu MA Jie ZHANG Junpei GUO Zhuo MA Chang YU Ying ZHANG Jinlong ZHANG 
Supported by National Natural Science Foundation of China(31802149);China Postdoctoral Science(2019M651985);Natural Science Foundation of Jiangsu Province(BK20180919);Scientific Research Fund of Nanjing General Hospital of Nanjing Military Command(2016033);Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)。
[Objectives]This study was conducted to obtain porcine gastrin-releasing peptide(GRP)fusion protein.[Methods]The constructed pET32a(+)-GRP plasmid was transformed into Escherichia coli BL21(DE3)competent cells to obta...
关键词：Gastrin-releasing peptide Vector construction Induced expression Fusion protein PIG 
Visualizing carboxyl-terminal domain of RNA polymerase Ⅱ recruitment by FET fusion protein condensates with DNA curtains
《Biophysics Reports》2022年第2期80-89,共10页Linyu Zuo Jiawei Ding Zhi Qi 
supported by the National Natural Science Foundation of China (32088101 and 31670762)
Many recent references show that living cells can form some membrane-less organelles by liquid–liquid phase separation(LLPS) of biomolecules, like proteins and nucleic acids. LLPS has been confirmed to link with many...
关键词：Liquid–liquid phase separation(LLPS) Biomolecular condensates Single-molecule biophysics DNA curtains In vitro transcription assay 
ImmunoPET imaging of amyloid-beta in a rat model of Alzheimer’s disease with a bispecific,brain-penetrating fusion protein
《Translational Neurodegeneration》2022年第1期70-83,共14页Gillian Bonvicini Stina Syvänen Ken G.Andersson Merja Haaparanta-Solin Francisco López-Picón Dag Sehlin 
the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie Grant Agreement No.813528,the Swedish Research Council(2017-02413,2018-02715,2021-1083 and 2021-03524),the Swedish Innovation Agency(2019-00106);Alzheimerfonden,Hjärnfonden,Hedlunds stiftelse,Torsten Söderbergs stiftelse,Åhlenstiftelsen,Stiftelsen för gamla tjänarinnor,Stohnes stiftelse,Magnus Bergvalls stiftelse,Konug Gustaf V:s och Drottning Victorias frimuarestiftelse,Åke Wibergs stiftelse and Turku University Hospital.The funding organizations did not take part in designing the study,in collecting,analysing,or interpreting the data,or in writing the manuscript.
Background:Hijacking the transferrin receptor(TfR)is an effective strategy to transport amyloid-beta(Aβ)immuno-positron emission tomography(immunoPET)ligands across the blood-brain barrier(BBB).Such ligands are more ...
关键词：ImmunoPET Transferrin receptor Alzheimer’s disease AMYLOID-BETA 
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