大豆异黄酮糖苷水解工艺的研究  

Hydrolysis of soybean isoflavone glycoside

作　　者：高荣海[1] 李长彪[1] 孟宪文[1] 刘长江[1] 张春红[1] 刘欣[1] 

机构地区：[1]沈阳农业大学食品学院,沈阳市110161

出　　处：《中国油脂》2007年第5期52-55,共4页China Oils and Fats

基　　金：辽宁省科技厅科技攻关课题(2004205001)

摘　　要：通过正交试验得到了大豆异黄酮糖苷水解为大豆异黄酮苷元的最佳工艺条件。最佳酸法水解工艺条件为:盐酸浓度3 mol/L,水解温度80℃,水解时间180 min,酸法水解率为81.31%;最佳酶法水解工艺条件为:pH 6.0,酶解温度38℃,酶解时间90 min,加酶量为0.9 mg(50 mg糖苷型大豆异黄酮提取物),酶法水解率为82.54%。酶法水解的效果优于酸法水解的效果。By orthogonal test, the optimum conditions for soybean isoflavone glycoside to be hydrolyzed into isoflavone aglycon were found. The optimum reaction conditions of acid hydrolysis of soybean isoflavone glycoside were as follows ; concentration of HCl 3 mol/L, temperature 80℃, time 180 min. Under the optimum conditions, the hydrolysis rate by HCl reached 81.31%. The optimum reaction conditions of enzymatic hydrolysis of soybean isoflavone glycoside were as follows ： pH 6.0, temperature 38℃, time 90 rain, enzyme dosage 0.9 mg （ based on 50 mg soybean isoflavone glycoside）. Under the optimum conditions,the hydrolysis rate by enzyme reached 82.54%. The effect of enzymatic hydrolysis was better than that of acid hydrolysis.

关 键 词：大豆异黄酮糖苷 酸水解 酶水解 大豆异黄酮苷元 

分 类 号：TS201.2[轻工技术与工程—食品科学]