胞外单宁酶纯化及酶学性质的研究  

Purification and Enzymatic Properties of Extracellular Tannase from Aspergillus niger

作　　者：王维维[1,2] 邱树毅[1,2] 谢晓莉[1,2] 吴鑫颖[3] 李广神[2,3] 何顺荣[2] 

机构地区：[1]贵州大学生命科学学院,贵州贵阳550025 [2]贵州省发酵工程与生物制药重点实验室,贵州贵阳550003 [3]贵州大学化学与化工学院,贵州贵阳550003

出　　处：《食品科学》2011年第13期239-243,共5页Food Science

基　　金：贵州省自然科学基金项目(黔科合J字[2008]2091号;黔科合J字[2010]2279号);贵州省科技攻关项目(黔科合GY字(2009)3022号)

摘　　要：采用黑曲霉B0201固态生料发酵生产胞外单宁酶,经过硫酸铵分级沉淀、DEAE-纤维素层析、葡聚糖G-150凝胶柱层析纯化,得到电泳纯的单宁酶,纯化倍数达到18.44。对该酶的性质研究表明:单宁酶的相对分子质量大小约为58800,该酶的最适反应温度是40℃,最适反应pH值是5.0;以PG为底物,米氏常数Km为1.08mmol/L,Vmax为104.1μmol/(L.min)。Extraction with sodium citrate-citric acid buffer, （NH4）2SO4 fractional precipitation, DEAE-cellulose column separation and Sephadex G-150 column purification were sequentially carried out to obtain an electrophoretically pure extracellular tannase from solid state cultures of AspergiUus niger B0201, with a purification fold of 18.44. The enzyme had a molecular weight of roughly 58800 and the optimum reaction temperature and pH were 40 ℃ and 5.0, respectively. When the substrate was propyl gallate （PG）, the Km was 1.08 mmol/L and the Vmax 104.1 μmol/（L · min）.

关 键 词：单宁酶 生料发酵 纯化 

分 类 号：Q815[生物学—生物工程]