层层自组装法制备百里香精油微胶囊条件优化  

Condition Optimization for Thyme Oil Microcapsules Prepared by Layer-by-Layer Self-Assembly Method

作　　者：黄晶[1] 申莉莉[1] 白友菊 马治灿 陈加平[1] 冯武[1] 

机构地区：[1]华中农业大学食品科学技术学院,湖北武汉430070

出　　处：《食品科学》2016年第2期51-57,共7页Food Science

基　　金：华中农业大学预研项目(52209-814010)

摘　　要：采用层层自组装的方法,以百里香精油乳化液纳米微粒作为核心,利用壳聚糖和海藻酸钠对百里香精油进行包埋;以司盘80和吐温60复配乳化剂亲水亲油平衡(hydrophilic lipophilic balance,HLB)值、用量及油乳比例3个因素进行实验,确定乳化液最适制备条件;以壁材溶液质量浓度、组装层数、每层组装时间、搅拌速率、组装温度、壁材溶液pH值和固化剂用量进行单因素试验,以主要因素设计正交试验,确定最佳包埋条件。结果表明,乳化液制备条件为HLB值10、乳化剂用量6%、精油与乳化剂体积比1∶6,此条件下所制备的乳化微粒为模板,组装层数4层、搅拌速率1 040 r/min、每层组装时间15 min、壁材溶液pH 5.0、组装温度30℃,此条件下包埋率最高为80.23%。In this work, using thyme oil emulsion nanoparticles as the core, chitosan（CS） and sodium alginate（ALG） were employed for embedding thyme essential oil by a layer-by-layer self-assembly method. Three emulsification conditions including HLB（hydrophilic lipophilic balance） value of 1：1 mixture of Span 80 and Tween 60, emulsifier concentration and ratio of oil to emulsifier were optimized to be 10, 6% and 1：6, respectively. The maximum microencapsulation efficiency of 80.23% was obtained when self-assembly of the emulsion particles in four layers by alternate use of CS and ALG was conducted for 15 min at 30 ℃ with stirring at a speed of 1 040 r/min, as determined by orthogonal array design.

关 键 词：层层自组装 微胶囊 百里香精油 正交试验 包埋率 

分 类 号：TS202.3[轻工技术与工程—食品科学]