海洋细菌JMUAZ5琼胶酶的酶学性质及酶解产物的抗氧化活性  

Characterization of Agarase from Marine Bacterial Strain JMUAZ5 and Antioxidant Activity of Enzymatic Hydrolysate

作　　者：赵蕊[1] 倪辉[1,2,3,4] 洪清林 肖安风[1,2,3,4] 蔡慧农[1,2,3,4] 朱艳冰[1,2,3,4] 

机构地区：[1]集美大学生物工程学院,福建厦门361021 [2]福建省食品微生物与酶工程重点实验室,福建厦门361021 [3]厦门市南方海洋研究中心经济海藻资源化利用与深加工重点实验室,福建厦门361021 [4]厦门市食品与生物工程技术研究中心,福建厦门361021 [5]绿麒(厦门)海洋生物科技有限公司,福建厦门361021

出　　处：《中国食品学报》2016年第1期61-68,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31371751);厦门南方海洋研究中心项目(13PZP002NF03;13GZP004NF10);福建省食品微生物与酶工程重点实验室开放基金项目(M20130906)

摘　　要：【目的】分离海洋来源的琼胶酶产生菌,确定其分类地位,研究其所产琼胶酶粗酶的酶学性质及酶解产物的抗氧化活性,为琼胶酶的进一步开发应用提供理论依据。【方法】利用以琼胶为唯一碳源的筛选培养基筛选分离产琼胶酶的菌株;采用16S r RNA基因序列分析确定菌株的分类地位;研究菌株所产琼胶酶粗酶的酶学性质;通过测定酶解产物的还原能力和清除自由基ABTS·+的能力,分析酶解产物的抗氧化活性。【结果】分离到一株产胞外琼胶酶的菌株JMUAZ5,根据16S r RNA基因序列分析,该菌株归属于弧菌属(Vibrio sp.);菌株JMUAZ5琼胶酶粗酶具有良好的底物专一性;最适反应温度为40°C,在20°C和25°C条件下稳定;最适反应p H值为8.0,在p H 6.0~10.0范围内稳定;Ca^(2+)和Al^(3+)对酶活性具有强烈促进作用,而Zn^(2+)和Fe^(3+)对酶具有强烈抑制作用;琼胶酶粗酶对测试的抑制剂、去垢剂和变性剂均具有好的抗性;酶解产物琼胶低聚糖具有还原能力和清除ABTS·+的能力。【结论】海洋细菌弧菌属JMUAZ5可产胞外琼胶酶,酶解产物有一定的抗氧化活性,具有潜在应用价值。[Objective] The aim is to identify a marine agarase-producing bacterium, characterize its crude enzyme and analyse the antioxidant activity of the enzymatic hydrolysate. [Method] The agar-degrading bacteria were isolated from marine mud by screening medium with agar as the sole carbon source. The strain was identified by the analyses of 16 S r RNA gene sequence. Its crude enzyme was characterized and the antioxidant activity of the enzymatic hydrolysate were analysed by detecting the reducing ability and scavenging power on ABTS radicals. [Result] 16 S rRNA phylogenetic analyses showed that the agarase-producing strain JMUAZ5 belonged to the genus Vibrio sp., which could produce extracellular agarase, its crude enzyme had significant substrate specificity. The optimum temperature and pH of strain JMUAZ5 agarase were 40 ℃ and 8.0, respectively. The enzymatic activity was stable at 20 ℃ and 25 ℃. It also showed stability across a pH range of 6.0-10.0. Ca^2＋and Al^3＋could strongly promote the agarase activity. Whereas, Zn^2＋ and Fe^3＋ significantly inhibited the enzyme activity. The crude enzyme had good resistance to detect inhibitors, detergents and denaturant. In addition, the enzymatic hydrolysate, agaro-oligosaccharides, had reducing ability and scavenging power on ABTS radicals. [Conclusion] Marine bacterium Vibrio sp. JMUAZ5 could produce extracellular agarase, its enzymatic hydrolysate had antioxidant activity and potential application values.

关 键 词：弧菌属 琼胶酶 酶学性质 抗氧化活性 

分 类 号：TS201.25[轻工技术与工程—食品科学]