2015年我国食源性副溶血性弧菌毒力基因及耐药特征研究  

Virulence and antimicrobial characteristics of foodborne Vibrio parahaemolyticus strains in China in 2015

作　　者：白瑶[1] 叶淑瑶[2] 闫韶飞[1] 韩海红[1] 余东敏[1] 李志刚[1] 杜春明[1] 李凤琴[1] BAI Yao YE Shu-Yao YAN Shao-Fei HAN Hai-Hong YU Dong-Min LI Zhi-Gang DU Chun-Ming LI Feng-Qin(Key Lab of Food Safety Risk Assessment of National Health and Family Planning Commission, China National Centre for Food Safety Risk Assessment, Beijing 100021, China College of Food Science and Engineering, Northwest Agriculture and Forestry University, Yangling 712100, China)

机构地区：[1]国家食品安全风险评估中心卫生部食品安全风险评估重点实验室,北京100021 [2]西北农林科技大学食品科学与工程学院,杨凌712100

出　　处：《食品安全质量检测学报》2017年第6期2318-2324,共7页Journal of Food Safety and Quality

基　　金：国家重点研发计划项目(2016YFD0401102)~~

摘　　要：目的探究我国2015年食源性副溶血性弧菌毒力基因分布情况和耐药特征。方法通过聚合酶链式反应,研究1046株副溶血性弧菌携带毒力基因tlh、tdh和trh情况,并采用微量肉汤稀释法,测定副溶血性弧菌对氨苄西林、阿莫西林/克拉维酸、头孢唑林、头孢噻肟、头孢他啶、亚胺培南、庆大霉素、四环素、环丙沙星、左氧沙星、氯霉素、复方新诺明等抗生素的敏感性。结果 1046株食源性副溶血性弧菌中有965株(92.3%)对一种或多种抗生素耐药,总耐药率为92.2%。全部菌株对庆大霉素和左氧氟沙星敏感,对其余10种抗生素有不同程度耐药,耐药率最高的前3位抗生素分别为头孢唑林(85.4%)、氨苄西林(59.4%)、阿莫西林/克拉维酸(49.3%)。1046株副溶血性弧菌全部携带tlh基因,其中19株(1.8%)trh基因阳性,4株(0.4%)tdh基因阳性。毒力基因阳性的所有菌株均未发现多重耐药现象,部分毒力基因阳性菌株对青霉素类和第一代头孢类抗生素耐药。结论我国2015年食源性副溶血性弧菌毒力基因携带率较低,对第一代头孢类和青霉素类抗生素耐药率较高;水产养殖地区应在养殖环节加强抗生素的管理并规范使用。Objective To evaluate virulence distribution and antimicrobial resistance of Vibrio parahaemolyticus isolated from food in China in 2015. Methods A polymerase chain reaction method was used to detect virulence genes of tlh, tdh and trh. The susceptibility of 1046 isolates of Vibrio parahaemolyticus to 12 antibiotics were determined by a broth microdilution susceptibility test. The antibiotics of ampicillin, amoxicillin-clavulanic acid, cefazolin, cefotaxime, ceftazidime, imipenem, gentamicin, tetracycline, ciprofloxacin, levofloxacin, chloramphenicoand trimethoprim/sulfamethoxazole were chosen. Results Totally 965 strains （92.2%） presented resistance to single or multiple antibiotics. All strains were sensitive to gentamicin and levofloxacin, and presented resistance to other antibiotics. The highest resistance rates were cefazolin （85.4%）, ampicillin （59.4%） and amoxicillin-clavulanic acid （49.3%）. All the 1046 isolates were positive to tlh gene, including 19 isolates were positive to trh gene and 4 isolates were positive to tdh gene. All the positive strains were not resistance to multiple antibiotics, and some of them were resistance to penicillins and the first-generation cephalosporin. Conclusion There is a lower positive rate of virulence genes in Vibrio parahaernolyticus isolated from food in China in 2015, though with a higher resistance rate to penicillins and the first-generation cephalosporin antibiotics. There is an urgent need to strengthen the management and standardization of antibiotics in a breeding process in the farming areas.

关 键 词：副溶血性弧菌 毒力 耐药 食品 

分 类 号：TS201.3[轻工技术与工程—食品科学]