云南高校图书馆联盟文献共享服务平台- 国家重点基础研究发展计划(2012CB720604)

国家重点基础研究发展计划(2012CB720604): 作品数：14被引量：13H指数：2; 导出分析报告; 相关作者：杨振军张礼和朱月洁马元关注更多>>; 相关机构：北京大学武汉大学更多>>; 相关期刊：《Science China Chemistry》《Virologica Sinica》《中国科学：化学》《Wuhan University Journal of Natural Sciences》更多>>; 相关主题：SIRNA修饰SYNTHESISPEPTIDECONJUGATES更多>>; 相关领域：医药卫生化学工程生物学更多>>

核酸适配体修饰与包载及其在疾病靶向性治疗和检测中的应用被引量：3: 《中国药物化学杂志》2019年第6期456-468,共13页王舒鹤周新洋朱月洁吴静杨振军; 科技部重大新药创制科技重大专项(2017ZX09303013);科技部973项目(2012CB720604).; 核酸适配体是一类能形成独特三维结构、长度较短的寡聚(脱氧)核苷酸,能与多种靶标分子特异性结合,具有与抗体类似的作用,在疾病的治疗、诊断等方面具有巨大潜力,但同时核酸适配体与其他寡聚核苷酸一样存在血清稳定性差、入胞困难、经肾...; 关键词：核酸类药物核酸适配体化学修饰

Selective alkylation and bioactivity of phosphorothioated nucleolin aptamer AS1411被引量：1: 《Journal of Chinese Pharmaceutical Sciences》2017年第1期23-30,共8页张光普邓家荔杨先桃朱月洁关注张礼和杨振军; the Ministry of Science and Technology of China(Grant No.2012CB720604);National Natural Science Foundation of China(Grant No.21332010); An operationally simple and efficient method for the synthesis of a wide range of alkylated nucleotides under mild conditions was developed. This improved method furnishes alkylated nucleotides fi＇om both single nucl...; 关键词：Phosphorothioated nucleotide ALKYLATION APTAMER AS 1411 NUCLEOLIN

Evaluation of the intracellular trafficking of siRNAs in A375 cells by confocal laser scanning microscopy: 《Journal of Chinese Pharmaceutical Sciences》2016年第12期859-868,共10页Yiping Diao Jing Sun Mengyi Yang Bo Xu Lihe Zhang Zhenjun Yang; Ministry of Science and Technology of China(Grant No.2012AA022501,2012CB720604);the National Natural Science Foundation of China(Grant No.20932001,81302626); Investigation intracellular trafficking of siRNAs following their delivery to cells is of great interest to elucidate dynamics of siRNA in cytoplasm. In this study, we present a novel confocal laser scanning microsco...; 关键词：siRNA/CLD complex Endosomal escape Cytoplasmic distribution Confocal laser scanning microscopy

siRNA缀合物的研究进展被引量：1: 《中国科学：化学》2016年第7期633-642,共10页孙晶杨振军; 国家自然科学基金重点课题(编号:20932001);国家重点基础研究发展计划(编号:2012CB720604);国家高技术研究发展计划(编号:2012AA022501)项目资助; 小干扰RNA(small interfering RNA,si RNA)能够对靶m RNA进行高效性、高特异性转录后调控,具有可传递性等特点,已被用于多种疾病的治疗研究.但其自身存在的诸多缺陷(如稳定性差、难以跨膜等)限制了它在临床上的广泛应用.对核苷酸单元进...; 关键词：小干扰RNA siRNA缀合物化学合成基因沉默

丙型肝炎病毒感染人肝细胞系对多种唾液酸转移酶和半乳糖基转移酶表达的影响被引量：1: 《中国生化药物杂志》2015年第10期5-10,共6页刘晓宇章晓联; 国家自然科学基金(21572173;31370197);湖北省医学领军人才计划(523-276003);973课题(2012CB720604); 目的探讨丙型肝炎病毒(hepatitis C virus,HCV)感染人肝癌细胞系Huh7.5.1细胞后与肿瘤相关的唾液酸转移酶家族和半乳糖基转移酶家族的35个糖基转移酶的表达变化。方法利用HCV感染的Huh7.5.1肝细胞模型,采用Western blot检测HCV感染后HC...; 关键词：丙型肝炎病毒唾液酸转移酶半乳糖基转移酶 HCV感染糖苷标志物

Serum ficolin-2 concentrations are significantly changed in patients with hepatitis B virus infection and liver diseases被引量：2: 《Virologica Sinica》2015年第4期249-260,共12页Tielong Chen Yilan Hu Quanquan Ding Jing Yu Fubing Wang Fengling Luo Xiao-Lian Zhang; supported by grants from the Natio nal Natural Science Foundation of China (31221061, 31270176 and 31370197);National Outstanding You th Foundation of China (81025008);the 973 Program of China (2012CB720604);the Program for Changjia ng Scholars and Innovative Research Team in Universi ty (IRT1030);the Hubei Province’s Outstanding Medical Academic Leader Program (523-276003);the Developm ent Fund for Collaborative Innovation Center of Glycosc ience of Shandong University;the Science and Tech nology Program of Wuhan (201150530141); Human ficolin-2 is an important lectin complement pathway activator that is secreted from liver cells and has been implicated as an anti-infection innate immune molecule. However, the role of ficolin-2 protein and its...; 关键词：ficolin-2 CHRONIC HEPATITIS B(CHB) HEPATOCELLULAR

茎环引物实时定量PCR技术用于检测异核苷修饰的siRNA的可行性研究被引量：1: 《中国科学：化学》2015年第7期710-718,共9页王雨斯马元刘欣洁张礼和杨振军; 国家重点基础研究发展计划(2012CB720604);国家高技术研究发展计划(2012AA022501);国家自然科学基金(20932001)资助; 本文考察了茎环引物实时定量PCR技术用于定量检测D-/L-异核苷(D-/L-iso NA)修饰的siRNA的可行性.根据阈值循环数(Ct值)及异核苷修饰的siRNA模板的初始浓度的对数值(lgC)绘制了标准曲线,与siRNA的标准曲线进行对比,发现D-异尿苷(D-isodU...; 关键词：茎环引物实时定量PCR 异核苷绝对定量双标准曲线法

Serum stability enhancement of siRNA caused by peptide conjugation at 3'-terminus of sense strand: 《Journal of Chinese Pharmaceutical Sciences》2014年第4期215-219,共5页邹朗黄野王晓锋马元刘洋关注张礼和杨振军; Ministry of Science and Technology of China(Grant No.2012CB720604);National Natural Science Foundation of China(Grant No.20932001); RNA interference has been widely used for gene therapy of various infectious diseases and malignant tumors. However, its poor stability in serum has limited further clinic application. Here, we found that stability of...; 关键词：SIRNA Serum stability Peptide conjugation NUCLEASES

Ficolins and infectious diseases被引量：3: 《Virologica Sinica》2014年第1期25-32,共8页Yushan Ren Quanquan Ding Xiaolian Zhang; supported by grants from the National Outstanding Youth Foundation of China(81025008);973 Program of China(2012CB720604);National Natural Science Foundation of China(31221061,31370197);National Grand Program on Key Infectious Disease(2012ZX10003002-015);the Hubei Province's Outstanding Medical Academic Leader Program,Changjiang Scholars and Innovative Research Team,the 211 program(303-581045);the Science and Technology Program of Wuhan(301274075);the FundamentalResearch Funds for the Central Universities; Ficolins are serum complement lectins,with a structure similar to mannose-binding lectin （MBL） and lung surfactant protein （SP）-A and SP-D.Ficolins activate the lectin complement system and play important roles in...; 关键词：L-FICOLIN H-ficolin M-ficolin VIRUS BACTERIA

Loss of silencing activity caused by 5′-terminal modification with D-/L-isonucleotide(isoNA) or locked nucleic acid(LNA) could not be restored by 5′-terminal phosphorylation被引量：1: 《Science China Chemistry》2014年第2期329-334,共6页HUANG Ye CHEN Zhuo WANG Zhuo LI YaTing CHEN Yue YANG ZhenJun ZHANG LiHe; supported by the National Natural Sciences Foundation of China(20932001);the Program of the Ministry of Science and Technology of China(2009ZX09503,2012AA022501,2012CB720604); In this study,a series of 5′-phosphorylated siRNAs with D-/L-isonucleotide(isoNA)or locked nucleic acid(LNA)incorporated at the 5′-terminus were synthesized.It was found that after incorporating either isoNA or LNA ...; 关键词：isonucleotide locked nucleic acid （LNA） 5＇-terminal phosphorylation

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