国家重点基础研究发展计划(2004CB19604)

作品数:17被引量:41H指数:3
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相关作者:华跃进田兵许镇坚华孝挺许光治更多>>
相关机构:浙江大学浙江大学医学院附属邵逸夫医院扬州大学更多>>
相关期刊:《中国农业科技导报(中英文)》《科学通报》《Science China(Life Sciences)》《核农学报》更多>>
相关主题:DEINOCOCCUS_RADIODURANS耐辐射球菌DEINOCOCCUS耐辐射奇球菌PPRI更多>>
相关领域:生物学环境科学与工程轻工技术与工程更多>>
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不同利福平浓度压力下耐辐射球菌的自发突变率与突变谱研究被引量:3
《核农学报》2010年第6期1166-1171,共6页华孝挺 王超 黄丽芬 李铭峰 王媛 陈玙 翁石莉 童艳铮 田兵 华跃进 
国家"973"项目(2004CB19604);国家"863"项目(2007AA021305);国家自然科学基金重点资助项目(30830006);农业部行业专项(200803034);浙江省科技厅项目(2006E10058)
根据耐辐射球菌(Deinococcus radiodurans)rpoB基因的保守性,建立了rpoB/Rifr突变分析系统。为研究不同利福平(rifampin,Rif)浓度对耐辐射球菌自发突变率和突变谱的影响,分别检测耐不同利福平浓度下辐射球菌的突变率。结果表明:在5μg/m...
关键词:DNA修复 突变 低浓度利福平 
Evolution of double MutT/Nudix domain-containing proteins: similar domain architectures from independent gene duplication-fusion events
《Journal of Genetics and Genomics》2009年第10期603-610,共8页Jun Lin Yihuai Hu Bing Tian Yuejin Hua 
supported by the National Basic Research Program of China (No. 2004CB19604);the National Hi-Tech Development Program (No. 2007AA021305);the National Natural Science Foundation of China (No.30830006);the Project "Application of Nuclear Techniques in Agriculture" from the Chinese Ministry of Agriculture (No. 200803034) to YJH.
The MutT/Nudix superfamily proteins repair DNA damage and play a role in human health and disease. In this study, we examined two different cases of double MutT/Nudix domain-containing proteins from eukaryotes and pro...
关键词:MutT/Nudix superfamily DNA repair gene duplication-fusion DROSOPHILA DEINOCOCCUS 
Engineering Deinococcus radiodurans into biosensor to monitor radioactivity and genotoxicity in environment被引量:2
《Chinese Science Bulletin》2008年第11期1675-1681,共7页GAO GuanJun FAN Lu LU HuiMing HUA YueJin 
the National Basic Research Program (Grant No. 2004CB19604);National Hi-Tech Development Program (Grant No. 2007AA021305);Distinguished Young Scientist (Grant No. 30425038);key project from the National Natural Science Foundation of China (Grant No. 30330020)
Based on a genetically modified radioresistant bacteria Deinococcus radiodurans, we constructed a real time whole cell biosensor to monitor radioactivity and genotoxicity in highly radioactive environ-ment. The enhanc...
关键词:生物传感器 环境监测 放射能 环境污染 
检测环境放射性和遗传毒性物质的耐辐射球菌生物传感器的构建被引量:1
《科学通报》2008年第8期932-938,共7页高冠军 范陆 陆辉明 华跃进 
国家重点基础研究发展计划(批准号:2004CB19604);国家高科技研究发展计划(批准号:2007AA021305);国家杰出青年科学基金(批准号:30425038);国家自然科学基金(批准号:30330020)资助项目
基于经遗传修饰的极端抗辐射细菌——耐辐射球菌,构建了一个实时全细胞生物传感器,以检测高放射性环境中的放射性物质和遗传毒物.把加强型绿色荧光蛋白(eGFP)连接到耐辐射球菌中一个可受DNA损伤诱导调控的关键基因——recA基因的启动子...
关键词:耐辐射球菌 生物传感器 EGFP RECA 遗传性毒物 DNA损伤 电离辐射 丝裂霉素C 
Functional analysis of the sbcD (dr1921) gene of the extremely radioresistant bacterium Deinococcus radiodurans被引量:1
《Chinese Science Bulletin》2007年第18期2506-2513,共8页HU YiHuai MA ChenYu TIAN Bing LIN Jun HUA YueJin 
Supported by the National Basic Research Program of China (Grant No. 2004CB19604);National Science Fund for Distinguished Young Scholars (Grant No. 30425038);Key Project from the National Natural Science Foundation of China (Grant No. 30330020)
In eukaryotes, the Mre11-Rad50-Nbs1 (MRN) complex, which resides at the crossroads of DNA repair and checkpoint signaling, rapidly forms prominent foci at damage sites following double-strand break (DSB) induction. Th...
关键词:辐射阻抗细菌 基因分析 泛函分析 DNA修补 
dr1127:A novel gene of Deinococcus radiodurans responsible for oxidative stress
《Chinese Science Bulletin》2007年第15期2081-2087,共7页WU YuanYuan TIAN Bing HUA YueJint 
the National Basic Research Program of China (Grant No. 2004 CB 19604);Grant for Distinguished Young Scientist of China (Grant No. 30425038);National Natural Science Foundation of China (Grant No. 30330020)
The functional analysis of dr1127,a novel gene in Deinococcus radiodurans was performed in this pa-per. The dr1127 gene was found occasionally in our microarray and 2-DE gel experiments. Mutation of the dr1127 gene de...
关键词:dr1127 异常基因 氧化损伤 基因突变 
极端耐辐射球菌sbcD基因(dr1921)功能分析被引量:1
《科学通报》2007年第17期2036-2042,共7页胡沂淮 马辰宇 田兵 林军 华跃进 
国家重点基础研究发展计划(批准号:2004CB19604);国家杰出青年科学基金(批准号:30425038);国家自然科学基金重点项目(批准号:30330020)资助
在真核生物中,Mre11-Rad50-Nbs1(MRN)复合体处于DNA修复和细胞周期调控的关键位置.当DNA双链断裂损伤诱导后,MRN复合体可快速与损伤部位结合,参与起始的DNA末端的处理.在MRN复合体中,任何一个亚基的基因突变都会引起生物体对DNA损伤超...
关键词:SBCD DNA 双链断裂修复 耐辐射球菌 
耐辐射球菌DNA修复机制研究新进展被引量:3
《中国农业科技导报》2007年第4期49-54,共6页黄丽芬 陆辉明 华孝挺 华跃进 
国家重点基础研究发展规划项目(2004CB19604);国家自然科学基金重点资助项目(30330020);国家杰出青年基金项目(30425038);浙江大学农业部核农学重点实验室开放基金;扬州大学校级基金资助
耐辐射球菌是迄今为止发现的最耐辐射的原核生物,是研究DNA损伤与修复的模式生物。根据国内外实验室和本实验在耐辐射球菌研究上取得的最新研究成果,本文从该细菌的结构特征、分子防御机制、重要修复基因、基因组学和蛋白质组学等方面...
关键词:耐辐射球菌 DNA修复 蛋白质组学 基因组学 
Ring-like nucleoid does not play a key role in radioresistance of Deinococcus radiodurans被引量:1
《Science China(Life Sciences)》2007年第4期525-529,共5页GAO GuanJun, LU HuiMing, YIN LongFei & HUA YueJin Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou 310029, China 
Supported by the National Basic Research Program of China (Grant No. 2004CB19604);the National Natural Science Foundation of China (Grant No. 30330020);the National Science fund for Distinguished Young Scholars (Grant No. 30425038)
The conclusion based on transmission electron microscopy, "the tightly packed ring-like nucleoid of the Deinococcus radiodurans R1 is a key to radioresistance", has instigated lots of debates. In this study, according...
关键词:DEINOCOCCUS radiodurans R1 RING-LIKE NUCLEOID PPRI ionizing irradiation RADIORESISTANCE 
Characterization of the double mutant of Deinococcus radiodurans lexA1 and lexA2
《Chinese Science Bulletin》2007年第8期1046-1052,共7页ZHOU Qing CHEN WeiWei ZHANG XinJue XU Hong XU BuJin HUA YueJin 
Supported by the National Natural Science Foundation of China(Grant No.30330020),;National Basic Research Program of China(Grant No.2004CB19604);and the State Funds for Distinguished Young Scientists of China(Grant No.30425038)
LexA and RecA play a crucial role in SOS response in Escherichia coli.In classical SOS response,LexA functions as a repressor to regulate the expression of many genes including RecA after DNA damage occurs.Previous st...
关键词:耐辐射球菌 双突变体 LexA1 LexA2 缺失性突变 
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