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国家自然科学基金(30371651): 作品数：20被引量：51H指数：4; 导出分析报告; 相关作者：何贤辉徐丽慧曾耀英查庆兵杜丽蕊更多>>; 相关机构：暨南大学暨南大学附属第一医院更多>>; 相关期刊：《Cellular & Molecular Immunology》《Acta Pharmacologica Sinica》《中国公共卫生》《免疫学杂志》更多>>; 相关主题：包涵体复性PD-L1原核表达小鼠更多>>; 相关领域：医药卫生生物学更多>>

3种流感病毒细胞毒T淋巴细胞抗原表位比较: 《中国公共卫生》2010年第10期1293-1294,共2页王飞鹏何贤辉; 国家自然科学基金(30572119和30371651); 关键词：流感病毒抗原表位表位预测交叉抗原反应

The immunosuppressive effect of gossypol in mice is mediated by inhibition of lymphocyte proliferation and by induction of cell apoptosis被引量：1: 《Acta Pharmacologica Sinica》2009年第5期597-604,共8页Wen-bin XU Li-hui XU Hong-song LU Dong-yun OU-YANG Huan-jing SHI Jing-fang DI Xian-hui HE; Acknowledgments This work was supported by the National Natural Science Foundation of China （No 30572199, No 30230350, No 30371651） and the Key Subject of Biochemistry and Molecular Biology of Guangdong Province. We thank Prof 03-xuan XIE for her kind help in capturing images; Dr Xi-chao WANG for performing the flow cytometry analysis; Chun-yong LIU, Xiao-ying WANG, He GUO, 03 GAO, and Yu-ting LI for their excellent technical assistance; and Zhi YANG and Man-lin YAO for kindly helping with preparation of the manuscript.; Aim： To investigate the immunosuppressive effect of gossypol in mice both in vitro and in vivo. Methods： The in vitro effect of gossypol on the proliferation of lymphocytes isolated from lymph nodes of BALB/c mice w...; 关键词：GOSSYPOL LYMPHOCYTES cell proliferation apoptosis delayed-type hypersensitivity

Construction of Soluble Mamu-B~*1703,a Class I Major Histocompatibility Complex of Chinese Rhesus Macaques,Monomer and Tetramer Loaded with a Simian Immunodeficiency Virus Peptide被引量：4: 《Cellular & Molecular Immunology》2009年第2期117-122,共6页Dongyun Ouyang Xiaoying Wang Xianhui He Lihui Xu Huanjing Shi Qi Gao He Guo; supported by Natural Science Fund of Guangdong Province (No.8451063201000340);the Talented Man Initiation Fund of Jinan University (No.51208004, No.51208017) to Dr. DY Ouyang ;grants from the National Natural Science Foundation of China (No.30572199, No.30230350 and No.30371651) to Prof. XH He;the Biochemistry and Molecular Biology Key Discipline of Guangdong Province.; Chinese-descent rhesus macaques have become more prevalent for HIV infection and vaccine investigation than Indian-origin macaques. Most of the currently available data and reagents such as major histocompatibility co...; 关键词：Mamu-B＊ 17 MHC class I rhesus macaque TETRAMER

Identification of major histocompatibility complex class I alleles in Chinese rhesus macaques被引量：4: 《Acta Biochimica et Biophysica Sinica》2008年第11期919-927,共9页Dongyun Ouyang Lihui Xu Zhengxi Dai Huanjing Shi Gaohong Zhang Yongtang Zheng Xianhui He; This work was supported by the grants from the National Natural Science Foundation of China （No. 30572199, No. 30230350 and No. 30371651） to Prof. XH He; the Talented Man Initiation Fund of Jinan University （No. 51208004 and No. 51208017） and Natural Science Fund of Guangdong Province （No. 8451063201000340） to Dr. DY Ouyang; the Scientific and Technological Projects of Yunnan （2004NG12 and 2006PT08）, the Major State Basic Research Development Program of China （2006CB504208）, the Knowledge Innovation Program of CAS （KSCX1-YW-R-15） to Dr. YT Zheng, and ＂Western Light＂ Projects to Dr. GH Zhang; as well as by the Biochemistry and Molecular Biology Key Discipline of Guangdong Province; Acknowledgements We thank Dr. Natasja de Groot, Dr. Nel Otting and IMGT Nonhuman Primate Nomenclature Committee for naming the Mamu-A and Mamu-B sequences.; Major histocompatibility complex （MHC） class I information is vital for understanding variance of immune responses in HIV vaccination and biomedical models. In this study, 9 Mamu-A and 13 Mamu-B alleles were identif...; 关键词：Mamu-A Mamu-B MHC class I rhesus macaque

抗人PD-1高亲和力抗血清的制备及其在PD-1免疫印迹分析中的应用: 《暨南大学学报（自然科学与医学版）》2008年第3期315-319,共5页施焕敬徐丽慧韩捷卢宏松何贤辉; 国家自然科学基金项目(30572199、30230350、30371651);生物化学与分子生物学广东省重点学科资助(); 利用纯化的可溶性人PD-1胞外域(sPD-1)蛋白为免疫原免疫小鼠,获得抗血清.免疫印迹显示该抗血清与原核表达的sPD-1有特异性反应,但与某些菌体蛋白存在交叉反应.经菌体蛋白吸附法处理后,交叉反应明显减弱.以商业化抗人PD-1抗体和小鼠sPD-...; 关键词：程序死亡蛋白-1 JURKAT T细胞抗血清免疫印迹

Phenotypic and Functional Analysis of LCMV gp33-41-Specific CD8 T Cells Elicited by Multiple Peptide Immunization in Mice Revealed the Up-regulation of PD-1 Expression on Antigen-Specific CD8 T Cells被引量：1: 《Cellular & Molecular Immunology》2007年第6期431-437,共7页Yi Liu Lihui Xu Yiqun Jiang Jianfang Sun Xianhui He; supported by the grants from the National Natural Science Foundation of China(No.30672474,No.30572199,No.30230350,and No.30371651);grants from Institute of Dermatology,Chinese Academy of Medical Sciences&Peking Union Medical College.; The phenotype and function of antigen-specific CD8 T cells are closely associated with the efficacy of a therapeutic vaccination. Here we showed that multiple immunizations with LCMV gp33-41 peptide （KAV） in Freund...; 关键词：LCMV CD8 T cell peptide vaccine phenotype PD-1

HLA-A^＊2402-BSP在大肠杆菌中的优化表达及其四聚体的制备和鉴定被引量：2: 《生物工程学报》2007年第2期284-291,共8页贾仟涛徐丽慧李丰耀查庆兵何贤辉; 国家自然科学基金重点项目资助(No.30230350);国家自然科学基金面上项目资助(No.30371651和30572199)~~; HLA-A*2402是中国人群中最常见的等位基因之一,为研究该基因型人群的人巨细胞病毒(HCMV)特异性细胞毒T细胞(CTL)免疫应答,需要制备负载相应抗原肽的HLA-A*2402四聚体。以RT-PCR方法克隆HLA-A*2402重链基因的cDNA,并构建了羧基端融合生...; 关键词：四聚体 HLA-A^＊2402 巨细胞病毒密码子优化原核表达包涵体

Preparation and Identification of HLA-A*1101 Tetramer Loading with Human Cytomegalovirus pp65 Antigen Peptide被引量：1: 《Cellular & Molecular Immunology》2007年第2期141-146,共6页Fengyao Li Lihui Xu Qingbing Zha Xiaoyun Chi Qiantao Jia Kianhui He; supported by grants from the National Natural Science Foundation of China(No.30230350,No.30572199 and No.30371651).; MHC/peptide tetramer technology has been widely used to study antigen-specific T cells, especially for identifying virus-specific CD8^＋ T cells in humans. The tetramer molecule is composed of HLA heavy chain, β2-mic...; 关键词：HLA-A＊ 1101 TETRAMER CYTOMEGALOVIRUS CD8^＋T cell EPITOPE

可溶性人sPD-L1及其抗体的制备和鉴定被引量：3: 《生物工程学报》2007年第1期106-111,共6页徐丽慧迟晓云李丰耀贾仟涛查庆兵何贤辉; 国家自然科学基金(No.30572199和30371651)资助项目~~; 通过固定化金属离子亲和层析进行柱上复性与纯化,获得高纯度的可溶性PD-L1胞外域(sPD-L1),其纯度达95%,纯化的sPD-L1经免疫印迹分析得到验证,并具有与其受体PD-1的特异性结合活性;以该抗原免疫小鼠获得高滴度的抗血清,并以制备的sPD-L1-...; 关键词：PD-L1 原核表达重折叠亲和层析抗体

人外周血淋巴细胞及单核细胞表达PD-L1和PD-L2的动力学研究被引量：2: 《中国病理生理杂志》2006年第5期873-877,共5页迟晓云何贤辉查庆兵徐丽慧曾耀英王通; 国家自然科学基金重点项目(No.30230350);国家自然科学基金面上项目(No.30371651);国家重点基础研究发展规划项目(No.G200057006); 目的:探讨PD-L1和PD-L2在正常人外周血静息和活化的B细胞、T细胞及单核细胞表面的表达规律。方法:利用荧光抗体染色和流式细胞术分析静息状态及经多克隆刺激剂脂多糖(LPS)和美洲商陆丝裂原(PWM)刺激6、24、48和72 h后表达PD-L1和PD-L2的...; 关键词：B7-H1 B7-DC 脂多糖类商陆有丝分裂原类流式细胞术淋巴细胞单核细胞

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