BACKGROUND As living biodrugs,mesenchymal stem cells(MSCs)have progressed to phase 3 clinical trials for cardiovascular applications.However,their limited immediate availability hampers their routine clinical use.AIM ...
Mitochondrial organelle transplantation (MOT) is an innovative strategy for the treatment of mitochondrial dysfunction such as cardiac ischemic reperfusion injuries, traumatic brain and spinal cord injuries, cerebral ...
supported by the National Natural Science Foundation of China(22078238,U23B20121)。
Ligament cryopreservation enables a prolonged shelf life of allogeneic ligament grafts,which is fundamentally important to ligament reconstruction.However,conventional cryopreservation techniques fail to eliminate the...
Cryopreservation plays an essential role in biobanking and cell therapy,but the physiological toxicity of traditional cryoprotectants such as glycerol and dimethyl sulfoxide(DMSO)has raised safety issues for biomedica...
supported by the National Natural Science Foundation of China(No.82172114);the Anhui Provincial Natural Science Foundation for Distinguished Young Scholars(No.2108085J37);the National Key Research and Development Program of China(2022YFC2703000).
With the rapid development of stem cell-related therapies and regenerative medicine,the clinical application of stem cell products is on the rise.However,ensuring the effectiveness of these products after storage and ...
Objective:To assess the effect of cryopreservation on stemness and proliferation potential of sheep spermatogonial stem cells(SSCs)in vitro.Methods:Sheep testicular cells were isolated and putative SSCs were enriched ...
supported by the National Key R&D Program of China(No.2017YFC1002003);the National Natural Science Foundation of China(No.81771637 and No.81571488);Shanghai Collaborative Innovation Center for Translational Medicine(No.TM202015 to ZJZ);the China Postdoctoral Science Foundation(No.2019M661521);the Key Project of Research and Development of Ningxia Hui Autonomous Region of China(No.2020BFH02002).
Cryopreservation of rare testicular-retrieved spermatozoa for intracytoplasmic sperm injection(ICSI)in patients with severe oligozoospermia and azoospermia remains a major challenge in clinical practice.This study eva...
This work was partially supported by grants from the US National Science Foundation(CBET-1831019 to XH and DGE-1840340 that is a GRFP fellowship to SS);National Institutes of Health(NIH R01EB023632 to XH).
Human induced pluripotent stem cells(hiPSCs)possess tremendous potential for tissue regeneration and banking hiPSCs by cryopreservation for their ready availability is crucial to their widespread use.However,contempor...
supported by the National Natural Science Foundation of China,Nos.82000871(to ZKC),81871495(to JSC);the Natural Science Foundation of Hunan Province,China,No.2020JJ5001(to ZKC);the Science Research Grant of Aier Eye Hospital Group,China,No.AF1913D2(to ZKC);Central South University Postdoctoral Funds,China。
In vertebrates, most somatosensory pathways begin with the activation of dorsal root ganglion(DRG) neurons. The development of an appropriate DRG culture method is a prerequisite for establishing in vitro peripheral n...
This work was supported by National Natural Science Foundation of China(No.31472054);the National Key Research and Development Program of China(2016YFC1000600).
Slow freezing is the most commonly used technique for the cryopreservation of spermatozoa in clinical practice.However,it has been shown to have a negative impact on sperm function and structure.Vitrification as a suc...