CRYOPRESERVATION

作品数:215被引量:407H指数:10
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相关领域:医药卫生农业科学更多>>
相关作者:李尚为王娟于媛王跃嗣焦飞更多>>
相关机构:四川大学上海理工大学滨州医学院华中农业大学更多>>
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相关基金:国家自然科学基金国家高技术研究发展计划中国博士后科学基金国家重点基础研究发展计划更多>>
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  • 学科=医药卫生—基础医学x
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Clinical experience with cryopreserved mesenchymal stem cells for cardiovascular applications:A systematic review
《World Journal of Stem Cells》2025年第3期124-138,共15页Moaz Safwan Mariam Safwan Bourgleh Khawaja Husnain Haider 
BACKGROUND As living biodrugs,mesenchymal stem cells(MSCs)have progressed to phase 3 clinical trials for cardiovascular applications.However,their limited immediate availability hampers their routine clinical use.AIM ...
关键词:CARDIOVASCULAR CRYOPRESERVATION HEART Mesenchymal stem cells Umbilical cord stem cells Randomized controlled trials Stem cells 
Cryopreserved Fibroblast and Mesenchymal Stem Cells (MSCs) Being Alternative Mitochondrial Donors for Mitochondrial Organelle Transplantation (MOT)
《Journal of Biomaterials and Nanobiotechnology》2024年第4期65-77,共13页Xianpeng Jiang Brent Segal Mark S. Kindy Catherine C. Baucom 
Mitochondrial organelle transplantation (MOT) is an innovative strategy for the treatment of mitochondrial dysfunction such as cardiac ischemic reperfusion injuries, traumatic brain and spinal cord injuries, cerebral ...
关键词:Mitochondria Mitochondrial Organelle Transplantation MOT CRYOPRESERVATION Fibroblasts MSCS 
Vitrification cryopreservation of ligaments based on zwitterionic betaine
《Chinese Journal of Chemical Engineering》2024年第8期1-9,共9页Liming Zhang Xinmeng Liu Haoyue Li Lei Zhang 
supported by the National Natural Science Foundation of China(22078238,U23B20121)。
Ligament cryopreservation enables a prolonged shelf life of allogeneic ligament grafts,which is fundamentally important to ligament reconstruction.However,conventional cryopreservation techniques fail to eliminate the...
关键词:LIGAMENT BETAINE VITRIFICATION CRYOPRESERVATION 
Melittin enhanced intracellular trehalose with synergistic membrane stabilization of macromolecular protectants for cell cryopreservation
《Science China(Technological Sciences)》2024年第4期1160-1169,共10页NIU QingJing GAO ShuHui ZHU KongYing REN LiXia YUAN XiaoYan 
Cryopreservation plays an essential role in biobanking and cell therapy,but the physiological toxicity of traditional cryoprotectants such as glycerol and dimethyl sulfoxide(DMSO)has raised safety issues for biomedica...
关键词:MELITTIN intracellular trehalose membrane stabilization macromolecular protectants cell cryopreservation 
Advanced cryopreservation engineering strategies:the critical step to utilize stem cell products被引量:1
《Cell Regeneration》2023年第1期119-132,共14页Xiaohu Wang Enyu Wang Gang Zhao 
supported by the National Natural Science Foundation of China(No.82172114);the Anhui Provincial Natural Science Foundation for Distinguished Young Scholars(No.2108085J37);the National Key Research and Development Program of China(2022YFC2703000).
With the rapid development of stem cell-related therapies and regenerative medicine,the clinical application of stem cell products is on the rise.However,ensuring the effectiveness of these products after storage and ...
关键词:Stem cell cryopreservation Photothermal rewarming Electromagnetic rewarming MICROENCAPSULATION Synergetic ice inhibition 
Cryopreserved ovine spermatogonial stem cells maintain stemness and colony forming ability in vitro
《Asian pacific Journal of Reproduction》2023年第6期273-280,共8页R.Kumar Pramod Deepthi Varughese A.Javed Jameel Bhisma Narayan Panda Soma Goswami Abhijit Mitra 
Objective:To assess the effect of cryopreservation on stemness and proliferation potential of sheep spermatogonial stem cells(SSCs)in vitro.Methods:Sheep testicular cells were isolated and putative SSCs were enriched ...
关键词:Spermatogonial stem cell Sheep STEMNESS CRYOPRESERVATION TRANSFECTION 
Clinical benefits of a modified Cryopiece system for cryopreservation of rare ejaculated and testicular spermatozoa for ICSI
《Asian Journal of Andrology》2022年第5期533-539,共7页Wei Chen Chuan Huang Peng Li Feng Liu Jian Sun Zi-Jue Zhu Jing Zhai Yuan Xu Yan Hong Jian-Lin Hu Yun-Peng Peng Zhen-Bo Zhang Yu Wu Zheng Li 
supported by the National Key R&D Program of China(No.2017YFC1002003);the National Natural Science Foundation of China(No.81771637 and No.81571488);Shanghai Collaborative Innovation Center for Translational Medicine(No.TM202015 to ZJZ);the China Postdoctoral Science Foundation(No.2019M661521);the Key Project of Research and Development of Ningxia Hui Autonomous Region of China(No.2020BFH02002).
Cryopreservation of rare testicular-retrieved spermatozoa for intracytoplasmic sperm injection(ICSI)in patients with severe oligozoospermia and azoospermia remains a major challenge in clinical practice.This study eva...
关键词:Cryopiece system intracytoplasmic sperm injection outcome rare sperm cryopreservation 
Sand-mediated ice seeding enables serum-free low-cryoprotectant cryopreservation of human induced pluripotent stem cells被引量:3
《Bioactive Materials》2021年第12期4377-4388,共12页Bin Jiang Weijie Li Samantha Stewart Wenquan Ou Baolin Liu Pierre Comizzoli Xiaoming He 
This work was partially supported by grants from the US National Science Foundation(CBET-1831019 to XH and DGE-1840340 that is a GRFP fellowship to SS);National Institutes of Health(NIH R01EB023632 to XH).
Human induced pluripotent stem cells(hiPSCs)possess tremendous potential for tissue regeneration and banking hiPSCs by cryopreservation for their ready availability is crucial to their widespread use.However,contempor...
关键词:SAND Ice seeding CRYOPRESERVATION iPSC Stem cell 
Characteristics of neural growth and cryopreservation of the dorsal root ganglion using three-dimensional collagen hydrogel culture versus conventional culture被引量:2
《Neural Regeneration Research》2021年第9期1856-1864,共9页Ze-Kai Cui Shen-Yang Li Kai Liao Zhi-Jie Wang Yong-Long Guo Luo-Sheng Tang Shi-Bo Tang Jacey Hongjie Ma Jian-Su Chen 
supported by the National Natural Science Foundation of China,Nos.82000871(to ZKC),81871495(to JSC);the Natural Science Foundation of Hunan Province,China,No.2020JJ5001(to ZKC);the Science Research Grant of Aier Eye Hospital Group,China,No.AF1913D2(to ZKC);Central South University Postdoctoral Funds,China。
In vertebrates, most somatosensory pathways begin with the activation of dorsal root ganglion(DRG) neurons. The development of an appropriate DRG culture method is a prerequisite for establishing in vitro peripheral n...
关键词:ANTI-APOPTOSIS collagen hydrogel CRYOPRESERVATION dorsal root ganglion NEUROGENESIS RNA-seq Schwann cell tissue engineering 
Improving native human sperm freezing protection by using a modified vitrification method被引量:2
《Asian Journal of Andrology》2021年第1期91-96,共6页Dai Zhou Xing-Ming Wang Rui-Xue Li Yi-Ze Wang Yuan-Chi Chao Zhi-Zhong Liu Zeng-Hui Huang Hong-Chuan Nie Wen-Bing Zhu Yue-Qiu Tan Li-Qing Fan 
This work was supported by National Natural Science Foundation of China(No.31472054);the National Key Research and Development Program of China(2016YFC1000600).
Slow freezing is the most commonly used technique for the cryopreservation of spermatozoa in clinical practice.However,it has been shown to have a negative impact on sperm function and structure.Vitrification as a suc...
关键词:CRYOPRESERVATION CRYOPROTECTANT slow freezing SPERM VITRIFICATION 
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