TWO-HYBRID

作品数:24被引量:35H指数:3
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相关领域:生物学医药卫生更多>>
相关作者:谢国红胡建英李功更多>>
相关机构:中国科学院北京大学厦门大学更多>>
相关期刊:《Science China(Life Sciences)》《World Journal of Gastroenterology》《Advances in Biological Chemistry》《Science Bulletin》更多>>
相关基金:国家自然科学基金国家重点基础研究发展计划国家高技术研究发展计划更多>>
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Construction of a Three-frame Yeast Two-hybrid cDNA Library of Fusarium oxysporum
《Journal of Northeast Agricultural University(English Edition)》2019年第2期25-32,共8页Luan Fei-shi Li Xiao-mei Zhu Zi-cheng Wang Xue-zheng 
Supported by the National Nature Science Foundation of China(31372088);the "Academic Backbone" Project of Northeast Agricultural University(15XG05);China Agriculture Research System(CARS-26-02)
A specialized test of two-hybrid library type three-frame cDNA yeast for Muskmelon Fusarium oxysporum using the switching mechanism at the 5'end of RNA template(SMART)technology was constructed to screen for interacti...
关键词:MELON FUSARIUM OXYSPORUM YEAST TWO-HYBRID cDNA library normalization 
Identification of anti-Gram-negative bacteria agents targeting the interaction between ribosomal proteins L12 and L10被引量:3
《Acta Pharmaceutica Sinica B》2018年第5期772-783,共12页Weiwei Wang Chao Liu Ningyu Zhu Yuan Lin JiANDong Jiang Yanchang Wang Yan Li Shuyi Si 
supported by the National Natural Science Foundation of China (Grant nos.81370089,81529003,81621064 and 81361138020);the Foundation for Innovative Research Groups and the Funds for International Cooperation and Exchange between China–Sweden and CAMS Initiative for Innovative Medicine (2016-12M-3-014)
Gram-negative bacteria have become the main pathogens and cause serious clinical problems with increased morbidity and mortality. However, the slow discovery of new antimicrobial agents is unable to meet the need for ...
关键词:YEAST TWO-HYBRID ESCHERICHIA COLI RIBOSOME L12/L10 Antimicrobial AGENTS 
Interaction of calcium- and integrin-binding protein 1 with integrin <i>α</i>11 and its possible involvement in pulmonary fibrosis
《Advances in Biological Chemistry》2014年第1期59-66,共8页Koji Yoshida Ah-Mee Park Shingen Ozaki Hiroshi Munakata 
Integrin α11 (ITGA11) is one of the collagen-binding integrin α chains;however, its biological significance remains unknown. To determine the functions of ITGA11, we performed a yeast two-hybrid screen using the cyt...
关键词:Calcium- and Integrin-Binding Protein 1 Fibrosis INTEGRIN α11 Yeast TWO-HYBRID 
Shared and discrete interacting partners of ELL1 and ELL2 by yeast two-hybrid assay被引量:2
《Advances in Bioscience and Biotechnology》2013年第7期774-780,共7页Fortuna Arumemi Ian Bayles Joshua Paul Christine Milcarek 
ELL2 (eleven-nineteen lysine-rich leukemia transcription elongation factor), a component of a larger complex with pTEFb (cyclin T and CDK9) and AF4, is up-regulated in plasma cells where it influences mRNA processing ...
关键词:TRANSCRIPTION ELONGATION IMMUNOGLOBULIN Synthesis YEAST TWO-HYBRID System 
Interaction of 14-3-3σ with KCMF1 suppresses the proliferation and colony formation of human colon cancer stem cells被引量:5
《World Journal of Gastroenterology》2013年第24期3770-3780,共11页Jian Zou Lin Mi Xiao-Feng Yu Jie Dong 
Supported by The Medical Guidance Projects of Shanghai Science Committee,No.10411961800;National Natural Science Foundation of China,No.81101617
AIM: To investigate the biological function of 14-3-3σ protein and to look for proteins that interact with 14-3-3σ protein in colon cancer stem cells. METHODS: Reverse transcription polymerase chain reaction was per...
关键词:14-3-3σ protein INTERACTING proteins YEAST TWO-HYBRID system COLON cancer stem cells 
Estrogen-related receptor γ disruption of source water and drinking water treatment processes extracts被引量:1
《Journal of Environmental Sciences》2011年第2期301-306,共6页Na Li Weiwei Jiang Kaifeng Rao Mei Ma Zijian Wang Satyanarayanan Senthik Kumaran 
supported by the National High Technology Research and Development Program (863) of China(No. 2007AA06Z414);the National Natural Science Foundation of China(No. 50778170)
Environmental chemicals in drinking water can impact human health through nuclear receptors. Additionally, estrogen-related receptors (ERRs) are vulnerable to endocrine-disrupting effects. To date, however, ERR disr...
关键词:drinking water estrogen receptor estrogen-related receptor two-hybrid yeast solid phase extraction 
In vitro demonstration of interactions among zinc-binding domains of cellulose synthases in Arabidopsis and aspen被引量:2
《Advances in Bioscience and Biotechnology》2010年第3期152-161,共10页Fuyu Xu Chandrashekhar P. Joshi 
Plant cellulose synthases (CesAs) are the key enzymes necessary for cellulose biosynthesis. In Arabidopsis, two distinct groups of three CesAs each are necessary for cellulose synthesis during primary and secondary ce...
关键词:ARABIDOPSIS Cellulose SYNTHASE POPLAR PROTEIN-PROTEIN Interaction Yeast TWO-HYBRID System ZINC-BINDING Domain 
Differential transcription-activating capability of NS1 proteins from different influenza virus subtypes expressed in yeast被引量:2
《Science China(Life Sciences)》2009年第6期545-550,共6页LI WeiZhong,WANG GeFei,ZENG Jun,ZHANG DanGui,ZHANG Heng,CHEN XiaoXuan,CHEN YouYing & Li KangSheng Department of Microbiology and Immunology,Shantou University Medical College,Shantou 515041,China 
Supported by National Natural Science Foundation of China(Grant No. 30771988);Funds for Outstanding Young Scientists in Universities of Guangdong Province (Grant No. LYM08056)
Influenza A virus NS1 protein is an important regulatory factor with multiple functions and contributes greatly to viral pathogenesis.In the present study,transcription-activating potential of NS1 from different influ...
关键词:INFLUENZA virus NS1 protein YEAST two-hybridation transcription-activation 
A Cdc2-related protein kinase hPFTAIRE1 from human brain interacting with 14-3-3 proteins被引量:3
《Cell Research》2006年第6期539-547,共9页Yankun Gao Mei Jiang Tao Yang Jian Ni Jiangye Chen 
grants from National Natural Science Foundation of China (Nos. 30400073 ,30330010).
hPFTAIRE1 (PFTK1), a Cdc2-related protein kinase, is highly expressed in human brain. It exhibits cytoplasmic distribution in Hela cells, although it contains two nuclear localization signals (NLSs) in its N-termi...
关键词:hPFTAIREI 14-3-3 proteins 14-3-3 binding motif TWO-HYBRID 
Interaction between Mnk2 and CBC^(VHL) ubiquitin ligase E3 complex
《Science China(Life Sciences)》2006年第3期265-273,共9页WANG Pingzhang, WANG Xin, WANG Feng, CAI Tianjing & LUO Ying Chinese National Human Genome Center, Beijing 100176, China Institute of Basic Medical Sciences, Chinese Acadamy of Medical Sciences, Beijing 100005, China Shanghai Genomics Inc., Shanghai 201203, China 
MAP kinase-interacting kinase-2 (Mnk2) is one of the downstream kinasesactivated by MAP kinases. It phosphorylates the eukaryotic initiation factor 4E (elF4E), althoughthe role of elF4E phosphorylation and the role of...
关键词:Mnk2  yeast two-hybrid  CBCVHL UBIQUITIN LIGASE E3 complex  VBP1. 
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