WHOLE-CELL

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Efficient biosynthesis of creatine by whole-cell catalysis from guanidinoacetic acid in Corynebacterium glutamicum
《Synthetic and Systems Biotechnology》2024年第1期99-107,共9页Chunjian Li Pengdong Sun Guoqing Wei Yuqi Zhu Jingyuan Li Yanfeng Liu Jian Chen Yang Deng 
funded by National Natural Science Foundation of China(no.32272279);the Key R&D project of Qingdao Science and Technology Plan(22-3-3-hygg-29-hy).
Creatine is a naturally occurring derivative of an amino acid commonly utilized in functional foods and pharmaceuticals.Nevertheless,the current industrial synthesis of creatine relies on chemical processes,which may ...
关键词：CREATINE Corynebacterium glutamicum Whole-cell biocatalysis Guanidinoacetate N-Methyltransferase Food additive 
Whole-cell one-pot biosynthesis of dodecanedioic acid from renewable linoleic acid
《Bioresources and Bioprocessing》2024年第1期753-762,共10页Yi-Ke Qi Jiang Pan Zhi-Jun Zhang Jian-He Xu 
the financial supports from the National Key Research and Development Program of China(Nos.2019YFA0905000&2022YFC2105900);National Natural Science Foundation of China(Nos.32071475&21536004).
Background Dodecanedioic acid(DDA),a typical medium-chain dicarboxylic fatty acid with widespread applications,has a great synthetic value and a huge industrial market demand.Currently,a sustainable,eco-friendly and e...
关键词：Linoleic acid Dodecanedioic acid Escherichia coli Whole-cell biosynthesis Multi-enzymatic cascade 
Review of construction methods for whole-cell computational models
《Systems Microbiology and Biomanufacturing》2022年第2期259-270,共12页Jingru Zhou Xingcun Fan Lingfeng Cao Huijie Sun Jianye Xia XueFeng Yan 
This work was fnancially supported by the National Key R&D Program of China(No.2019YFA0904300).
The complex mechanisms of the internal operation of cellular functions have not been fully resolved and these functions are regulated by multiple effects,such as transcription regulation,signal transduction,and enzyme...
关键词：Whole-cell computational model Systems biology STEADY-STATE Dynamic modeling 
Significantly improved oxidation of bio-based furans into furan carboxylic acids using substrate-adapted whole cells
《Journal of Energy Chemistry》2020年第2期20-26,共7页Mao Wen Xue-Ying Zhang Min-Hua Zong Ning Li 
financially supported by the National Natural Science Foundation of China(21676103);the Natural Science Foundation of Guangdong Province(2017A030313056);the Science and Technology Project of Guangzhou City(201804010179)。
Furan carboxylic acids are important building blocks in polymer and fine chemical industries. In this work, a simple substrate adaptation strategy was applied to improve the catalytic performances of Comamonas testost...
关键词：5-HYDROXYMETHYLFURFURAL 5-hydroxymethyl-2-furancarboxylic acid OXIDATION SUBSTRATE adaptation strategy WHOLE-CELL biocatalysis 
Enantioselective Resolution of(R,S)-Carvedilol to(S)-(2)-Carvedilol by Biocatalysts
《Natural Products and Bioprospecting》2017年第1期171-179,共9页Swetha Ettireddy Vijitha Chandupatla Ciddi Veeresham 
Authors are thankful for the financial assistance(SR/SO/HS/0087/2010)given by Science and Engineering Research Board,DST,New Delhi,India.Authors are also thankful to Symed labs Ltd.,Medak,India,for the kind gift of racemic Carvedilol.
Among the microorganisms employed in the study,Aspergillus niger(GUFCC5443),Escherichia coli(ATCC9637),Streptomyces halstedii(CKM-2),Pseudomonas putida(NCIB9494),Cunninghamella elegans(NCIM689)and Sphingomonas paucimo...
关键词：CARVEDILOL Whole-cell microorganisms Triacylglycerol lipase IMMOBILIZATION STEREOINVERSION 
Effect of the ginsenoside Rb1 on the spontaneous contraction of intestinal smooth muscle in mice被引量：3
《World Journal of Gastroenterology》2012年第38期5462-5469,共8页Lei Xu Sui-Ping Huang 
Supported by The National Natural Science Foundation of China, No. 30873328;The State Administration of Traditional Chinese Medicine of the People’s Republic of China, No. 06-075930
AIM: To investigate the effect and the possible mechanism of ginsenoside Rb1 on small intestinal smooth muscle motility in mice. METHODS: Intestinal smooth muscle strips were isolated from male ICR mice (5 wk old), an...
关键词：Ginsenoside Rb1 Intestinal smooth muscle Intestinal smooth muscle cell Potassium channel Spontaneous contraction Whole-cell patch clamp technique 
Whole-cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for rapid identification of bacteria cultured in liquid media被引量：4
《Science China(Life Sciences)》2011年第1期48-53,共6页ZHOU Na WANG Na XU Bin WANG Jie FANG JunJian DONG FangTing HE Kun YANG XiaoHong 
supported by the National Science and Technology Major Project (Grant No. 2008ZX10207);the Innovation Method Fund of Ministry of Science and Technology of China (Grant Nos. 2008IM022000 and 2009IM030300);the National Science and Technology Support Program (Grant No. 2008BAK41B0)
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been used for many years to rapidly identify whole bacteria. However, no consistent methodology exists for the rapid iden...
关键词：MALDI-TOF MS BACTERIA PCA identification 
A.Flavus Mediated Biotransformations for the Syntheses of Active A-oxyfunctionalized Compounds
《复旦学报（自然科学版）》2007年第5期739-,共1页P.Ayhan S.Betul Sopaci A.S.Demir 
1 Results Biotransformations are enzyme- and whole cell-catalysed conversions of non-natural substrates to products.They are important tools in organic synthesis,especially for the syntheses of chiral molecules,where ...
关键词：A.flavus whole-cell reaction BIOTRANSFORMATION α-hydroxy ketones 
WHOLE-CELL CLAMP STUDY OF XENOPUS EMBRYONIC CHOLINERGIC NEURONS
《Science China Chemistry》1989年第2期148-154,共7页谢佐平 王堤 竺煜杰 
Project supported by the National Natural Science Foundation of China
Whole-cell clamped myoballs are placed into direct visible contact with the growth cones of isolated neurons in embryonic Xenopus culture to serve as probe of acetylchollne (AcCHo) release in order to determine whethe...
关键词：WHOLE-CELL CLAMP CHOLINERGIC neurous repetitive firings XENOPUS embryo. 
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