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作品数:350被引量:414H指数:8
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相关领域:农业科学医药卫生更多>>
相关作者:母秋华齐志涛王资生王爱民刘新春更多>>
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Insights on evolution of virulence and resistance from the whole-genome analysis of a predominant methicillin-resistant Staphylococcus aureus clone sequence type 239 in China
《Chinese Science Bulletin》2014年第11期1104-1112,共9页Hongbin Chen Xi Yang Qi Wang Chunjiang Zhao Henan Li Wenqiang He Xiaojuan Wang Feifei Zhang Zhanwei Wang Minjun Chen Baoli Zhu Hui Wang 
supported,in part,by the Beijing Natural Science Foundation(7102130);the Program for New Century Excellent Talents in University(NCET-10-0205);the National Natural Science Foundation of China(31100106);Key Projects in the National Science&Technology Pillar Program(2012EP001002)
Earlier, we reported that ST239 was the15-year predominant methicillin-resistant Staphylococcus aureus(MRSA) clone in China. In this study, MRSA strain CN79 belonging to ST239 and isolated from blood was used to deter...
关键词:金黄色葡萄球菌 基因组分析 毒力基因 进化过程 序列类型 中国 克隆 GENBANK 
Tracing the location of powdery mildew resistance-related gene Stpk-V by FISH with a TAC clone in Triticum aestivum–Haynaldia villosa alien chromosome lines被引量:2
《Chinese Science Bulletin》2013年第33期4084-4091,共8页YANG XueMing CAO AiZhong SUN YuLei CHEN PeiDu 
supported by the National Basic Research Program of China(2009CB118304);the National Natural Science Foundation of China(31171540,30871519);the Program for New Century Excellent Talents in University(NCET-10-0496);the Independent Innovation of Agricultural Sciences(CX(11)1025);the Sci&Tech Project in Jiangsu Province(BE2011306)
Bacterial artificial chromosomes(BACs)or yeast artificial chromosomes(YACs)containing large inserts as probes for fluorescence in situ hybridization(FISH)have been used in the physical mapping of specific DNA sequence...
关键词:异源染色体 相关基因 物理位置 抗白粉病 簇毛麦 小麦族 TAC 跟踪 
Characterization and cloning of a brittle culm mutant (bc88) in rice (Oryza sativa L.)被引量:11
《Chinese Science Bulletin》2013年第24期3000-3006,2914,共7页RAO YuChun YANG YaoLong XIN DeDong LI XiaoJing ZHAI KaiEn MA BoJun PAN JianWei QIAN Qian ZENG DaLi 
supported by the National Natural Science Foundation of China (30971760, 31201183);the Zhejiang Provincial Qianjiang Talents Program of China ( 2010R10085);the Zhejiang Program of Education Department (Y201225687)
This study characterizes a brittle culm (bc88) mutant of rice (Oryza sativa L.) obtained by ethylene methylsulfonate (EMS)-induced mutagenesis of Wuyunjing 7. The bc88 mutant exhibits a diversity of pleiotropic phenot...
关键词:RICE (Oryza SATIVA L.) BRITTLE CULM Wuyunjing 7 CLONE mutation 
Clone of Chinese Jinan red-cross yellow cattle and evaluation of reproductive characteristics of cloned calf
《Chinese Science Bulletin》2005年第22期2592-2597,共6页DAI Yunping LIAN Zhengxing ZHU Huabin GONG Guochun WANG Lili WANG Haiping ZHAO Zhihui ZHU Qinghong FEI Jing LI Ning 
This work was supported by“863” High-Tech Research and Development Program (Grant No. 2001AA213091 & 2002AA206111) ; Natural Science Foundation of Bejjing (Grant No. 5030001 ).
Somatic cell clone technology is a viable ap- proach to preserving endangered livestock and wildlife ge- netic resources. In the present research, somatic cell nuclear transfer (SCNT) was performed using granulose cel...
关键词: 繁殖技术 肉体细胞 克隆技术 遗传基因 
Genetic analysis on 3'- terminal flanking region of uncoupling protein 3 in different pig breeds被引量:2
《Chinese Science Bulletin》2002年第18期1541-1543,共3页FANG Meiying, ZHAO Xingbo, LI Ning & WU ChangxinCollege of Animal Science and Technology, China Agricultural University, Beijing 100094, China State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100094, China 
This work was supported by the National Natural Science Foundation of China (Grant No. 30000119); the National High Technology Research ; Development Program of China (Grant No. 2001AA213121).
The 3’-terminal flanking region of porcine uncoupling protein 3 (UCP3) was cloned, the sequence data revealed 15 nucleotide substitutions among Landrace and three Chinese native pig breeds named Neijiang, Minpig and ...
关键词:PIG UNCOUPLING protein gene CLONE PCR-RFLP. 
Molecular cloning,in vitro expression and enzyme activity analysis of violaxan-thin de-epoxidase from Oryza sativa L.被引量:1
《Chinese Science Bulletin》2002年第11期915-917,共3页Lin, RC Li, LB Kuang, TY 
This work was supported by the State Key Basic Research ; Development Plan of China (Grant No. 1998010100);the Innovation Foundation of Laboratory of Photosynthesis Basic Research, Institute of Botany, the Chinese Academy of Sciences.
The violaxanthin de-epoxidase gene was cloned from rice (Oryza sativa subsp. japonica). The full length of the cDNA is 1887 bp, encoding a 446-amino acids protein with the transit peptide of 98 amino acids. The bacter...
关键词:Oryza SALIVA L. VIOLAXANTHIN de-epoxidase CLONE ex-pression enzyme activity. 
Cloning and analysis of a cDNA encoding acetohydroxy acid isomeroreductase from G2 pea被引量:4
《Chinese Science Bulletin》2001年第21期1808-1812,共5页XU Huasong XU Yunjian GU Xuesong HU Tingzhang SU Yanhui ZHU Yuxian 
This work was supported by the National Science Foundation for Distinguished Young Scholars (Grant No. 39725002); and the National "863" High-Tech Project (Grant No. 101-01-01-02).
Using cDNA representational difference analysis (cDNA RDA) method, we have successfully isolated a gene fragment whose expression was specifically induced by external GAa application. Screening a G2 pea cDNA library u...
关键词:G2 PEA CLONE acetohydroxy ACID isomeroreductase PROKARYOTIC expression. 
Construction of cDNA library from iron-deficiency induced maize roots and screening and identification of iron-stress gene Fdr3被引量:4
《Chinese Science Bulletin》2000年第12期1127-1131,共5页YIN Liping QI Xiaoting LIU Xianglin HUO Chunyan BAI Yanxia QIU Zesheng ZHANG Fusuo 
To isolate Fe-deficient related (Fdr) genes, an expression cDNA library of 4.5×105 pfu/μg has been constructed from maize roots in iron-stress. 6 clones have been screened from the cDNA library by differential hybri...
关键词:maize iron-deficient cDNA library differential hybridization SCREENING iron-deficient related (fdr) clone. 
Cloning and expression of a cDNA encoding ribosomal protein S4 from Rice (Oryza sativa)
《Chinese Science Bulletin》2000年第2期168-173,共6页QU Lijia LI Donghui ZHANG Yi LIU Meihua GU Hongya CHEN Zhangliang 
A cDNA clone, pS4, has been isolated from a cDNA library prepared from rice anthers of about 1.0 mm in length. DNA sequence analysis and database search show that the cDNA encodes a protein which is highly homologous ...
关键词:RIBOSOMAL protein S4 cDNA CLONE monocotyledonous plant tissue specificity(rice) 1) The NUCLEOTIDE SEQUENCE data reported is in the EMBL NUCLEOTIDE SEQUENCE Database under the ACCESSION number Y15009. 
Isolation of a vernalization-related cDNA clone(VRC) using mRNA differential display in winter wheat被引量:1
《Chinese Science Bulletin》1998年第14期1201-1205,共5页Dazhong Zhao Min Chen Kang Chong Li Wan Hualiang Huang Kehui Tan 
theNationalNaturalScienceFoundationofChina (GrantNo .39330 0 10 );theFoundationofChineseAcademyofSciencestoProf.TanKehui
Vernalization is an essential factor which affects the flowering development in cold_requiring plants. There is a key stage of nucleic acid and protein metabolism in the process of vernalization in winter wheat. To pr...
关键词:mRNA differential display vernalization_related cDNA CLONE WINTER wheat. 
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