AGROBACTERIUM-MEDIATED

作品数：38被引量：113H指数：7
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相关机构：东北林业大学复旦大学中国农业大学西南大学更多>>
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A fast and genotype-independent in planta Agrobacterium-mediated transformation method for soybean
《Plant Communications》2024年第12期17-29,共13页Heng Zhong Changbao Li Wenjin Yu Hua-ping Zhou Tara Lieber Xiujuan Su Wenling Wang Eric Bumann Rafaela Miranda Lunny Castro Yaping Jiang Wening Gu Qingli Liu Brenden Barco Chengjin Zhang Liang Shi 
The information described in this article is covered by two Syngenta patent publications:WO20211108336 and WO2021108337.
Efficient genotype-independent transformation and genome editing are highly desirable for plant biotechnology research and product development efforts.We have developed a novel approach to enable fast,high-throughput,...
关键词：SOYBEAN genotype-flexible transformation genome editing in planta regeneration herbicide selection 
Development of an Agrobacterium-mediated CRISPR/Cas9 gene editing system in jute(Corchorus capsularis)
《The Crop Journal》2024年第4期1266-1270,共5页Shaolian Jiang Qin Li Xiangxue Meng Mengxin Huang Jiayu Yao Chuanyu Wang Pingping Fang Aifen Tao Jiantang Xu Jianmin Qi Shuangxia Jin Liwu Zhang 
supported by the National Natural Science Foundation of China (31771369);the Natural Science Foundation of Fujian, China (2023J01443);the China Agriculture Research System of the Ministry of Agriculture and MARA (CARS-16)
Jute(Corchorus capsularis L.)is the second most important natural plant fiber source after cotton.However,developing an efficient gene editing system for jute remains a challenge.In this study,the transgenic hairy roo...
关键词：JUTE Agrobacterium-mediated transformation Genome editing Hairy root system 
Enemies atpeace:Recentprogressin Agrobacterium-mediated cereal transformation
《The Crop Journal》2024年第2期321-329,共9页Shaoshuai Liu Ke Wang Shuaifeng Geng Moammar Hossain Xingguo Ye Aili Li Long Mao Karl-Heinz Kogel 
financial assistance provided by the High-End Foreign Expert Recruitment Program(G2022051003L);National Natural Science Foundation of China(32201878);Hainan Yazhou Bay Seed Lab(B21HJ0215);Agricultural Science and Technology Innovation Program of CAAS(CAASZDRW202002,CAAS-ZDRW202201);Hebei Natural Science Foundation(C2021205013);Long Mao is also a“Yellow River Delta Scholar”in Sino-Agro Experimental Station for Salt Tolerant Crops(SAESSTC),Dongying,Shandong,China.
Agrobacterium tumefaciens mediated plant transformation is a versatile tool for plant genetic engineering following its discovery nearly half a century ago.Numerous modifications were made in its application to increa...
关键词：Agrobacterium tumefaciens Cereal species Genome editing Genetic engineering Plant breeding 
Development of an Agrobacterium-mediated CRISPR/Cas9 system in pea(Pisum sativum L.)被引量：3
《The Crop Journal》2023年第1期132-139,共8页Guan Li Rong Liu Rongfang Xu Rajeev KVarshney Hanfeng Ding Mengwei Li Xin Yan Shuxian Huang Juan Li Dong Wang Yishan Ji Chenyu Wang Junguang He Yingfeng Luo Shenghan Gao Pengcheng Wei Xuxiao Zong Tao Yang 
the financial support of the China Agriculture Research System of MOF and MARA-Food Legumes(CARS-08);the Agricultural Science and Technology Innovation Program(ASTIP)of the Chinese Academy of Agricultural Sciences。
Pea(Pisum sativum L.)is an annual cool-season legume crop.Owing to its role in sustainable agriculture as both a rotation and a cash crop,its global market is expanding and increased production is urgently needed.For ...
关键词：Agrobacterium-mediated transformation CRISPR/Cas9 PEA Genome editing 
Regeneration and Agrobacterium-mediated genetic transformation of twelve Eucalyptus species被引量：3
《Forestry Research》2022年第1期149-156,共8页Tingting Zhou Ying Lin Yan Lin Jianzhong Luo Jihua Ding 
supported by the National Natural Science Foundation of China(31971676);the Opening Project of State Key Laboratory of Tree Genetics and Breeding(K2020103);the Fundamental Research Funds for Central Universities(2662019PY007)。
Eucalyptus is a genus of over 900 species and hybrids,and many of them are valuable fast-growing hardwoods.Due to its economic importance,Eucalyptus is one of the early tree species whose genomes were deciphered.Howev...
关键词：REGENERATION transformation ACTIVATION 
Establishment of an efficient regeneration and genetic transformation system for Malus prunifolia Borkh. ‘Fupingqiuzi’
《Journal of Integrative Agriculture》2022年第9期2615-2627,共13页LIU Yu-song WANG Hong-ying ZHAO Yong-juan JIN Yi-bo LI Chao MA Feng-wang 
supported by the National Key Research and Development Program of China(2019YFD1001403-4);the Key S&T Special Projects of Shaanxi Province,China(2020zdzx03-01-02);the earmarked fund for the China Agriculture Research System of MOF and MARA(CARS-27)。
Malus prunifolia Borkh. ‘Fupingqiuzi’ has significant ecological and economic value and plays a key role in germplasm development and resistance research. However, its long juvenile phase and high heterozygosity are...
关键词：Malus prunifolia Borkh.‘Fupingqiuzi’ regeneration system Agrobacterium-mediated transformation system 
Establishment of A Simple and Efficient Agrobacterium-mediated Genetic Transformation System to Chinese Cabbage(Brassica rapa L.ssp.pekinensis)被引量：7
《Horticultural Plant Journal》2021年第2期117-128,共12页Xiaonan Li Haiyan Li Yuzhu Zhao Peixuan Zong Zongxiang Zhan Zhongyun Piao 
the National key research and Development Program(Grant No.2017YFD0101802);the National Natural Science Foundation of China(Grant Nos.31772326 and 31701930);China Postdoctoral Science Foundation(Grant Nos.2016M601345 and 2019T120219).
Chinese cabbage,belonging to Brassica rapa species,is an important vegetable in Eastern Asia.It is well known that Chinese cabbage is quite recalcitrant to genetic transformation and the transgenic frequency is genera...
关键词：Chinese cabbage Agrobacterium tumefaciens genetic transformation 
A Highly Efficient Agrobacterium-Mediated Method for Transient Gene Expression and Functional Studies in Multiple Plant Species被引量：6
《Plant Communications》2020年第5期129-140,共12页Youjun Zhang Moxian Chen Beata Siemiatkowska Mitchell Rey Toleco Yue Jing Vivien Strotmann Jianghua Zhang Yvonne Stahl Alisdair R.Fernie 
supported by funding from the Max Planck Society(A.R.F.and Y.Z.);the European Union’s Horizon 2020 project PlantaSYST SGA-CSA no.739582 under FPA no.664620(A.R.F.and Y.Z.).
Although the use of stable transformation technology has led to great insight into gene function,its application in high-throughput studies remains arduous.Agro-infiltration have been widely used in species such as Ni...
关键词：transient expression agro-infiltration subcellular localization protein-protein interaction 
Agrobacterium-mediated delivery of CRISPR/Cas reagents for genome editing in plants enters an era of ternary vector systems被引量：2
《Science China(Life Sciences)》2020年第10期1491-1498,共8页Yu Zhang Qiang Zhang Qi-Jun Chen 
Supported by grants from the National Crop Breeding Fund (2016YFD0101804);the National Natural Science Foundation of China (31872678 and 31670371);and the National Transgenic Research Project (2016ZX08009002).
Lack of appropriate methods for delivery of genome-editing reagents is a major barrier to CRISPR/Cas-mediated genome editing in plants.Agrobacterium-mediated genetic transformation(AMGT)is the preferred method of CRIS...
关键词：genome editing CRISPR/Cas ternary vector system delivery Agrobacterium-mediated transformation 
Marker gene excision in transgenic Brassica napus via Agrobacterium-mediated Cre/lox transient expression system
《Oil Crop Science》2020年第2期96-103,共8页Pandi Wang Xiaojuan Xiong Ping Fu Gang Wu Fang Liu 
supported by the National Program of Transgenic Variety Development of China(2018ZX08010-05B and 2019ZD080018);the Central Public-interest Scientific Institution Basal Research Fund;Major Research Project of CAAS Science。
Oilseed rape(Brassica napus L.)is one of the most important oil crops in the world.However,study on marker-free transgene of B.napus for bio-safety purpose is limited in this allotetraploid crop.In order to advance ma...
关键词：Brassica napus MARKER-FREE CRE/LOX Cross Auto-excision Herbicide resistance 
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