云南高校图书馆联盟文献共享服务平台- CHAIN

CHAIN: 作品数：1276被引量：1740H指数：17; 导出分析报告; 相关领域：理学医药卫生更多>>; 相关作者：韩俊林李晓松丛方媛王彩玲师义民更多>>; 相关机构：四川大学中国科学技术大学武汉大学浙江大学更多>>; 相关期刊：更多>>; 相关基金：国家自然科学基金国家重点基础研究发展计划国家高技术研究发展计划国家社会科学基金更多>>

M1磁珠富集联合重组酶介导的聚合酶链式反应技术检测肺炎克雷伯菌: 《中国科技期刊数据库医药》2024年第8期147-152,共6页张盼盼帖彦清; 一直以来,人类健康都在承受着病原微生不同程度的威胁,其中肺炎克雷伯菌是常见的条件致病菌之一,当机体免疫力低下时,容易发生炎性病变,从而引起肺炎、尿路感染和败血症等院内感染。综合分析肺炎克雷伯菌的致病特征,认为快速、准确地从...; 关键词：肺炎克雷伯菌 M1磁珠重组酶介导聚合酶链式反应 (polymerase CHAIN reaction PCR)

Identification of a Target Region for Specific Molecular Diagnosis of Human Papillomavirus Subtype 16: 《Journal of Pharmacy and Pharmacology》2019年第11期557-563,共7页Ketly Rodrigues Barbosa dos Anjos Anderson Alves da Silva Bezerra Ana Lisa do Vale Gomes; Objective: To identify a target region for molecular diagnosis from the E6 region of the Human papillomavirus (HPV). Method: This was a quantitative data research. The sample was composed of sequences from the Nationa...; 关键词：Papillomaviridae POLYMERASE CHAIN reaction COMPUTATIONAL BIOLOGY

Laboratory diagnosis of schistosomiasis mansoni: Current status and future trends: 《Asian Pacific Journal of Tropical Medicine》2019年第6期243-249,共7页Amira Taman Samar N.El-Beshbishi; Schistosomiasis is a neglected tropical disease that affects about 290 million patients worldwide. Children aged between 5 and 14 years represent 45.8% of the affected patients, in addition, schistosomiasis has been r...; 关键词：SCHISTOSOMA MANSONI ELISA miRNA TEGUMENT POLYMERASE CHAIN REACTION

Potentiating activity of rhein in targeting of resistance genes in methicillin-resistant Staphylococcus aureus被引量：2: 《Asian Pacific Journal of Tropical Medicine》2019年第1期14-18,共5页Ryong Gong Dae Young Lee Jae Won Lee Doo Jin Choi Geum-Soog Kim Sang Hyuk Lee Young-Seob Lee; carried out with the support of "Cooperative Research Program for Agriculture Science & Technology Development(Project No.PJ01191902)" Rural Development Administration,Republic of Korea; Objective: To investigate the synergistic effect between rhein(RHE) and oxacillin against Staphylococcus aureus(MRSA) at the gene level. Method: A minimum inhibitory concentration and checkerboard dilution test were c...; 关键词：Antimicrobials Mechanism of action STAPHYLOCOCCUS AUREUS POLYMERASE CHAIN reaction

一种快速检测婴幼儿配方奶粉中阪崎克罗诺杆菌的方法被引量：7: 《食品与发酵工业》2018年第10期212-218,共7页王蕊杨鑫焱陈思涵潘瑞丽吴霜满朝新姜毓君; 国家高层次人才特殊支持计划(810098)。; 对磁珠吸附过程中三羟甲基氨基甲烷-乙二胺四乙酸(Tris-EDTA,TE)缓冲液的p H值、蛋白酶K添加量、吸附时间和吸附温度等参数进行了优化。在优化条件下氨基磁珠可以非特异性地直接吸附婴幼儿配方奶粉中存在的脱氧核糖核酸(deoxyribonuclei...; 关键词：氨基磁珠婴幼儿配方奶粉阪崎克罗诺杆菌聚合酶链式反应(polymerase CHAIN reaction PCR) 脱氧核糖核酸(deoxyribonucleic acid DNA)

Molecular cloning, characterization, and promoter analysis of the isochorismate synthase （AalCS1） gene from Artemisia annua被引量：1: 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2017年第8期662-673,共12页Lu-yao WANG Ying ZHANG Xue-qing FU Ting-ting ZHANG Jia-wei MA Li-da ZHANG Hong-mei QIAN Ke-xuan TANG Shan LI Jing-ya ZHAO; supported by the National High-Tech R&D Program(863)of China(No.22011AA100605); Isochorismate synthase(ICS) is a crucial enzyme in the salicylic acid(SA) synthesis pathway. The full-length complementary DNA(cDNA) sequence of the ICS gene was isolated from Artemisia annua L. The gene, named AaICS...; 关键词：Salicylic acid ARTEMISIA annua L. Quantitative real-time POLYMERASE CHAIN reaction (qRT-PCR) Isochorismate SYNTHASE

Molecular and Phenotypical Characterization of <i>Mannheimia haemolytica</i>Isolated from Goats in Baghdad Province被引量：2: 《Advances in Microbiology》2017年第4期304-314,共11页Waffa A. Ahmed Roua J. Mohammed Ilham A. Khalaf; Mannheimia haemolytica (M. haemolytica) is a gram negative bacterium which can infect humans and animals. It’s commensal as a normal flora of the nasopharynx and tonsils in cattle, sheep and goats, pneumonic pasteure...; 关键词：Mannhimia haemolytica Goats POLYMERASE CHAIN Reaction (PCR) Baghdad

Detection of coat protein gene of nervous necrosis virus using loopmediated isothermal amplification被引量：2: 《Asian Pacific Journal of Tropical Medicine》2016年第3期230-235,共6页Jinik Hwang Sung-Suk Suh Mirye Park Myung-Joo Oh Jong-Oh Kim Sukchan Lee Taek-Kyun Lee; supported by the Korea Institute of Ocean Science&Technology(No.PE99315); Objective:To establish a novel and highly specific loop-mediated isothermal amplification(LAMP) assay for the identification of nervous necrosis virus(NNV) infection.Methods:A set of synthesized primers was used to ma...; 关键词：Nervous NECROSIS VIRUS Nodaviridae POLYMERASE CHAIN reaction Loop-mediated ISOTHERMAL AMPLIFICATION

Rapid and quantitative detection of hepatitis B virus被引量：6: 《World Journal of Gastroenterology》2015年第42期11954-11963,共10页Yue-Ping Liu Chun-Yan Yao; Supported by National Natural Science Foundation of China,No.81371885; Despite availability of a universal vaccine, hepatitis B virus(HBV) infection has a huge impact on public health worldwide. Accurate and timely diagnosis ofHBV infection is needed. Rapid developments have been made in...; 关键词：HEPATITIS B VIRUS BIOSENSOR POLYMERASE CHAIN react

Evaluation of a multiplex PCR assay for detection of cytomegalovirus in stool samples from patients with ulcerative colitis被引量：4: 《World Journal of Gastroenterology》2015年第44期12667-12675,共9页Saifun Nahar Atsushi Iraha Akira Hokama Ayako Uehara Gretchen Parrott Tetsuya Ohira Masatoshi Kaida Tetsu Kinjo Takeshi Kinjo Tetsuo Hirata Nagisa Kinjo Jiro Fujita; AIM: To evaluate a multiplex PCR assay for the detection of bacterial and viral enteropathogens in stool samples from patients with ulcerative colitis(UC). METHODS: We prospectively analyzed 300 individuals, including...; 关键词：POLYMERASE CHAIN reaction Ulcerativecolitis Cytome

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