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作品数:956被引量:1822H指数:15
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Cloning of BjNAC102 Promoter and Construction of Expression Vector in Brassica juncea
《Agricultural Science & Technology》2024年第3期14-20,共7页XIAO Xin-bo YUAN Yu-hui YOU Liang HUANG Xin CHEN Ming-zhe MA Yin-hua LIU Xian-jun CHEN Hu 
Supported by Natural Science Foundation of Hunan Province(2023JJ50083,2023JJ50084);Excellent Youth Project of Hunan Provincial Department of Education(22B0844);Hunan Provincial Graduate Research Innovation Project(CX20231274)。
Transcription factor NAC102 plays an important role in the abiotic stress responses of plants.In this study,the promoter sequence of 3000 bp located in the upstream of the BjNAC102 gene was cloned from Brassica juncea...
关键词:Brassica juncea PROMOTER BjNAC102 Plant expression vector construction 
Cloning of the ZoWRKY1 Gene and its Correlation with 6-gingerol Biosynthesis in Zingiber officinale Roscoe
《Agricultural Science & Technology》2021年第1期54-60,共7页TANG Jian-min QI Li-wang HE Xiao-ting JIANG Yu-xin LI Zhe-xin 
重庆市教育委员会科学技术研究项目(KJQN201901302);重庆市自然科学基金(cstc2019jcyj-msxmX0697)。
Ginger(Zingiber officinale Roscoe)has high economic value as medicinal and food resources.6-gingerol is the core medicinal constituents of ginger.In the present study,a local ginger cultivar of Chongqing was taken as ...
关键词:GINGER WRKY transcription factor 6-gingerol synthesis CORRELATION 
Characterization of MyDNMT1 and Changes of Global DNA Methylation during the Embryonic Development in Mizuhopecten yessoensis
《Agricultural Science & Technology》2017年第11期2147-2154,共8页许艺迪 刘卫东 滕伟鸣 于佐安 
Supported by Earmarked Fund for Modern Agro-industry Technology Research System of China(CARS-49);Natural Science Foundation of Liaoning Province(201602408)~~
DNA methylation is a critical epigenetic mechanism that influences gene transcription, genomic stability, X-chromosome inactivation and other factors, and appropriate DNA methylation is crucial in development. DNA met...
关键词:Mizuhopecten yessoensis DNA methylation DNA (cytosine-5) methyl- transferase 1 CLONING Global DNA methylation 
Cloning and Sequence Analysis of CHS Gene Fragment from Acer truncatum
《Agricultural Science & Technology》2016年第7期1658-1662,1678,共6页马立敏 丰震 齐新玲 
Supported by Agricultural Improved Variety Project of Shandong Province(LKZ[2014]No.96)
[Objective] This study aimed to clone and analyze the sequence of CHS gene from Acer truncatum leaves. [Method] Using A. truncatum cultivars No.1-6 as experimental materials, total RNA was extracted from A. truncatum ...
关键词:Acer truncatum Chalcone synthase CLONING Sequence analysis RT- PCR 
Cloning of Atr MYB Transcription Factor Gene from Acer truncatum
《Agricultural Science & Technology》2016年第4期792-796,821,共6页王延玲 郝雪英 丰震 
Supported by Agricultural Elite Cultivar Project of Shandong Province(lkz2014[96])~~
[Objective] Cloning of the AtrMYB transcription factor gene from Acer truncatum was conducted to further explore the red leaf development mechanism and breed cultivars of colored-leaf maple. [Method] The Acer truncat...
关键词:Acer truncatum MYB transcription factor Gene cloning 
Cloning and Molecular Identification of A Fatty Acid Desaturase 2 Gene in a and C Genome of Brassica Species
《Agricultural Science & Technology》2016年第5期1048-1054,共7页Lei CHEN Sang SHUANG Song CHEN Feng CHEN Qi PENG 
Supported by the National Natural Science Foundation of China (31301357);the Natural Science Foundation of Jiangsu Province,China (BK20130719)
The fatty acid desaturase 2(fad2) gene was proven to be a major locus for high oleic acid(C18:1).Brassica napus is an amphidiploid species originating from a spontaneous hybridization of Brassica rapa and Brassica ole...
关键词:Fad2 Gene SNP Brassica napus GENOTYPING 
Cloning and Bioinformatics Analysis of zmERECTA
《Agricultural Science & Technology》2016年第4期819-821,共3页Yihong JI Jinbao PAN Min LU Jun HAN Zhangjie NAN Qingpeng SUN 
Supported by the Distinguished Young Scientists Project of Beijing(CIT&TCD201304096);Academic Degrees and Graduate Education Reform and Development Program of Beijing University of Agriculture(5056516002\016)
[Objective] This study was conducted to clone zmERECTA gene according to Arabidopsis ERECTA sequence and predict its characteristics by bioinformatics. [Method] The c DNA of zmERECTA gene was isolated from B73 using R...
关键词:Zea mays BIOINFORMATICS CLONE 
Cloning and Bioinformatics Analysis of ZmERECTA-LIKE1 and Construction of Plant Expression Vector
《Agricultural Science & Technology》2016年第3期523-525,共3页Yihong JI Jinbao PAN Min LU Jun HAN Zhangjie NAN Qingpeng SUN 
Supported by the Distinguished Young Scientists Project of Beijing(CIT&TCD201304096);Academic Degrees and Graduate Education Reform and Development Program of Beijing University of Agriculture(5056516002\016)
[Objective] This study was conducted to clone and analyze ERECTA-LIKE1 gene in Zea mays by PCR and bioinformatics methods and to construct plant expression vector p Cambia3301-zm ERECTA-LIKE1. [Method] zm ERECTA-LIKE1...
关键词:Zea mays BIOINFORMATICS Plant expression vector 
Cloning and Bioinformatics Analysis of zmPKC
《Agricultural Science & Technology》2016年第1期42-44,160,共4页纪艺红 张彦彦 潘金豹 于涌鲲 卢敏 韩俊 南张杰 孙清鹏 
Supported by Beijing Natural Science Foundation(5132006)~~
[Objective] Protein kinase C(PKC) plays a vital role in signal transmission,stomatal regulation, cell proliferation and differentiation. This study was to clone zm PKC and predict its characteristics by bioinformati...
关键词:Zea mays zm PKC BIOINFORMATICS 
Cloning and Characterization of Phytochrome A Gene Fa PHYA from Tall Fescue
《Agricultural Science & Technology》2015年第9期1851-1856,共6页王小利 吴佳海 舒健虹 蔡一鸣 刘晓霞 李小冬 
Supported by National Natural Science Foundation of China(31360576)~~
The sequence fragment of PHYA, obtained from transcriptome sequencing,was used as the template, and the full-length c DNA sequence of PHYA gene in tall fescue was amplified using 3'RACE and 5'RACE techniques. The c ...
关键词:Tall fescue Phytochrome A gene CLONING BIOINFORMATICS 
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