分步酶解制备鲢鱼抗氧化肽的工艺研究  

Study on preparation of Hypophthalmichthys molitrix antioxidant peptides by two-step-hydrolysis

作　　者：陈轩[1] 周坚[1] 李吉绪[1] 陈季旺[1] 王琦[1] 张圣洁[1] 

机构地区：[1]武汉工业学院食品科学与工程学院,湖北武汉430023

出　　处：《食品工业科技》2012年第15期181-186,共6页Science and Technology of Food Industry

基　　金：国家高技术研究发展计划(863计划)项目(2010AA023003)

摘　　要：用分步酶解法制备鲢鱼抗氧化肽,首先从碱性蛋白酶、中性蛋白酶、胰蛋白酶、风味蛋白酶中筛选出碱性蛋白酶作为第一步酶解用酶,以水解度作评价指标,并得到酶解工艺条件:底物浓度15%,pH9.0,温度50℃,加酶量为24AU/kg。再从剩下的三种酶中筛选出胰蛋白酶与碱性蛋白酶复配,作为第二步酶解用酶,用响应面法进一步优化酶解工艺,考虑到成本和工艺的要求,最终确定的酶解工艺为:碱性蛋白酶水解时间为59.12min,胰蛋白酶水解时间为82.24min,两种酶的添加比例为1:1.48,即碱性蛋白酶添加量为24AU/kg,胰蛋白酶的添加量为35.5AU/kg。最终得到的鲢鱼抗氧化肽可溶性氮70.84%,多肽69.38%,水分4.21%,灰分25.67%;多肽浓度为2mg/mL时对DPPH自由基清除率为19.33%。In order to obtain Hypophthalmichthys molitrix antioxidant peptides,two-step-hydrolysis methodology was used.First of all,Alcalase was chosen as the enzyme for the first-step-hydrolysis from Alcalase,Flavourzyme,Trypsin and Neutrase.Degree of hydrolysis（DH）was tested by pH-stat method.Results indicated that the optimal processing conditions for hydrolyzing Hypophthalmichthys molitrix protein hydrolysate of Alcalase were protein concentrations 15%,pH9.0,50℃,ratio of Alcalase to silver carp protein with 24AU/kg.Then Trypsin was chosen from Flavourzyme,Trypsin and Neutrase as the enzyme for the second-step-hydrolysis.DPPH free radical scavenging ratio as response values was analyzed with response surface methodology.With the factors such as product cost and process requirement considered,optimum conditions to achieve the maximum antioxidant ability were as follows：Alcalase enzymolysis time 59.12min,Trypsin enzymolysis time 82.24min,Alcalase to Hypophthalmichthys molitrix protein with 24AU/kg,Trypsin to silver carp protein with 35.5AU/kg.The results of analyzing components showed that Hypophthalmichthys molitrix antioxidant peptides made under the same hydrolysis conditions contained 70.84% of soluble N,69.38% of peptides,4.21% of water and 25.67% of ash.DPPH free radical scavenging ratio was 19.33% when the peptide concentration was 2mg/mL.

关 键 词：鲢鱼 抗氧化肽 分步酶解 响应面 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]