冻藏对海湾扇贝闭壳肌蛋白质变性及组织结构的影响  

Effects of frozen storage on protein denaturation and structure of Argopecten irradiams muscle

作　　者：崔瑞颖[1] 焦学芹[1] 崔波[1] 祖铁红[1] 郑乾魏[1] 张志胜[1] 

机构地区：[1]河北农业大学食品科技学院,河北保定071000

出　　处：《食品工业科技》2013年第22期298-301,共4页Science and Technology of Food Industry

基　　金：国家海洋局海洋公益性行业科研专项(201205031)

摘　　要：以海湾扇贝闭壳肌盐溶性蛋白含量、肌原纤维蛋白Ca2＋-ATPase活性及组织的显微镜观察为指标，研究了冻藏条件对海湾扇贝闭壳肌蛋白质变性及组织结构的影响。结果表明，冷藏过程中蛋白质发生变性，盐溶性蛋白及肌原纤维蛋白Ca2＋ATPase活性显著下降（p〈0．05）；贮藏15～75d时间内，（-35±1）℃下的闭壳肌盐溶性蛋白含量高于（-25±1）℃，且有显著差异（P〈0．05）；贮藏过程中，（-35±1）℃下闭壳肌的肌原纤维蛋白Ca2＋-ATPase活性高于（-25±1）℃，除贮藏0d、45d差异不显著外。其余相同贮藏时间内不同冻藏温度对肌原纤维蛋白Ca2＋-ATPase活性影响差异显著（p〈0．05）。（-35±1）℃冻藏条件下海湾扇贝闭壳肌肌肉纤维相对（-25±1）℃冻藏组织结构更加完整。因此，选择（-35＋1）℃冻藏海湾扇贝闭壳肌对蛋白质及组织结构的变化影响较好。Changes of salt solubility protein,the Ca2＋-ATPase activity in myofibrillar protein and the structure of Argopecten irradiams muscle were studied during storage at two different temperatures（ （-25±1）℃, （-35±1）℃. Results showed that during frozen storage,salt solubility protein and the Ca2＋-ATPase activity in myofibrillar protein was reduction signifienatly（p〈0.05）. Argopecten irradiams muscle storage at two different temperatures （（-25±1）℃, （-35±1）℃））,salt solubility protein changed signifienatly during storage time at 15~75d（p〈0.05）. In the process of storage,the Ca2＋-ATPase activity in myofibrillar protein changed signifienatly at different frozen storage temperatures except storage time at 0d and 45d （p〈0.05）. Structure of Argopecten irradiams muscle frozen storage temperatures at （-35±1）℃ was more complete than the storage temperatures at （-25±1）℃. So （-35±1）℃ was better on protein denaturation and structure of Argopecten irradiams muscle.

关 键 词：冻藏 海湾扇贝 闭壳肌 蛋白质 结构 

分 类 号：TS254.4[轻工技术与工程—水产品加工及贮藏工程]