BIOLISTIC

作品数:15被引量:72H指数:5
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相关领域:农业科学生物学更多>>
相关作者:王新中于新智布都会朱建楚张磊更多>>
相关机构:西北农林科技大学阿维斯塔金格兰技术有限公司南京农业大学更多>>
相关期刊:《云南大学学报(自然科学版)》《Progress in Natural Science:Materials International》《Science China(Life Sciences)》《陕西农业科学》更多>>
相关基金:中国博士后科学基金国家高技术研究发展计划国家自然科学基金更多>>
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Development and drought tolerance assay of marker-free transgenic rice with OsAPX2 using biolistic particle-mediated co-transformation
《The Crop Journal》2017年第4期271-281,共11页Dan Feng Yanwei Wang Jinxia Wu Tiegang Lu Zhiguo Zhang 
supported by the National Major Project for Developing New GM Crops (2016ZX08001-003)
Abiotic stresses such as drought, salinity, and low temperature cause–losses in rice production worldwide. The emergence of transgenic technology has enabled improvements in the drought resistance of rice plants and ...
关键词:Rice PARTICLE bombardment-mediated CO-TRANSFORMATION MARKER-FREE Drought-tolerance 
Current and future editing reagent delivery systems for plant genome editing被引量:20
《Science China(Life Sciences)》2017年第5期490-505,共16页Yidong Ran Zhen Liang Caixia Gao 
Many genome editing tools have been developed and new ones are anticipated; some have been extensively applied in plant genetics, biotechnology and breeding, especially the CRISPR/Cas9 system. These technologies have ...
关键词:genome editing DNA RNA RNP and virus delivery Agrobacterium-mediated transformation biolistic method protoplast transfeetion ttransgene-free genome edited plant 
Biolistic Genetic Transformation of a Wide Range of Chinese Elite Wheat(Triticum aestivum L.)Varieties被引量:4
《Journal of Genetics and Genomics》2015年第1期39-42,共4页Kang Zhang Jinxing Liu Yi Zhang Zhimin Yang Caixia Gao 
funded by the Ministry of Agriculture of China(Nos.2014ZX0801003B and 2013ZX08002-004)
Wheat (Triticum aestivum L.) is a major staple food crop worldwide. It is economically important because it can be grown in a wide range of climates and geographic regions, and it has made an enormous contribution t...
关键词:VARIETIES Biolistic Genetic Transformation of a Wide Range of Chinese Elite Wheat Triticum aestivum L gene 
Multiple transgenes Populus xeuramericana 'Guariento' plants obtained by biolistic bombardment被引量:10
《Chinese Science Bulletin》2007年第2期224-230,共7页Wang JianGe Su XiaoHua Ji LiLi Zhang BingYu Hu ZanMin Huang RongFeng Tian YingChuan 
A JERF36 regulation gene, a selection marker gene (NPT-II), and the foreign genes levansucrase (SacB), Vitreoscilla hemoglobin (vgb), and Binary coleopterus insect resistance (BtCry3A+OC-I) were co-transferred into Po...
关键词:森林 生态系统 全球变暖 二氧化碳 植被 
Biolistic PDS—1000/He基因枪的使用方法被引量:5
《陕西农业科学》2003年第6期81-82,共2页朱建楚 布都会 于新智 王新中 
杨凌农业生物技术育种中心资助项目
基因枪广泛应用于生物基因转化。对基因枪使用中材料准备、微弹制备、DNA微载体制备、基因枪操作步骤、方法及注意事项进行全面介绍。同时推荐了不同类型组织细胞轰击的条件及参数 。
关键词:基因枪 基因转化 DNA微载体 操作步骤 
Studies of Improving the Frequency of Indica Rice Transformation by Biolistic Bombardment被引量:1
《High Technology Letters》2003年第1期6-10,共5页易自力  Wang Li  Cao Shouyun  Yuan Longping  Guan Chunyun  Zhau Puhan  Tian Wenzhang  Chu Chengcai  
the Chinese 863 High-Technology Program(NO.863-101-01-01-01)and Chinese Post-Doctoral Science Foundation(2001-14)
In order to improve the frequency of indica rice transformation by biolistic bombardment, suitable culture conditions for embryonic calli,an optimal selection scheme for resistant calli and seedlings, and optimum bomb...
关键词:indica rice biolistic bombardment TRANSFORMATION CALLUS 
Study on the Obtaining of Transgenic Wheat with GNA Alien Gene by Biolistic Particle被引量:1
《Agricultural Sciences in China》2002年第1期25-29,共6页XU Qiong-fang, LI Lian-cheng, CHEN Xiao, TIAN Fang, MA You-zhi, YE Xing-guo, ZHANG Zeng-yan, XU Hui-jun and XIN Zhi-yong( Key Laboratory of Agricultural Ministry for Crop Genetics and Breeding, Institute of Crop Breeding and Cultivation ,Chinese Academy of Agricultural Sciences, Beijing 100081 ) 
The immature embryos of wheat plants, cv. Jing 411, 12 - 14 days after pollination, were cultured on SD2 medium for callus induction. After 10 days culture, 800 wheat calli were bombarded by biolistic particle coated ...
关键词:Galanthus nivalis agglutinin bar gene Particle bombardment Transgenic wheat 
Comparison of Biolistic and Agrobacterium-mediated Transformation Methods on Transgene Copy Number and Rearrangement Frequency in Rice被引量:11
《Acta Botanica Sinica》2001年第8期826-833,共8页程在全 黄兴奇 Ray WU 
SupportedbygrantsfromtheRockefellerFoundation (RF98_0 0 1_60 6)andtheUnitedStatesDepartmentofAgriculture (USDA) ( 95_3730 0_1 5 80 ) . Presentaddress :MolecularBiologyLaboratory ;BiotechnologyResearchInstituteofYunnanProvince ;Kunming 65 0 2 2 3;Chi
Transferring foreign DNA into plant cells by biolistic and Agrobacterium _mediated methods may result in random integration of different copy numbers of the transgene, and different proportions of intact vs. rearra...
关键词:DNA rearrangement gene expression cassette Agrobacterium  _mediated method biolistic method rice (  Oryza sativa  ) 
Inheritance and expression of multiple disease and insect resistance genes in transgenic rice被引量:2
《Chinese Science Bulletin》2001年第2期101-106,共6页Daorong Feng Xinping Xu Guohua Qiu Baojian Li 
the State "863" High-Tech Project and the specific fund for rice of The South China Life Science and Technology Research Center from The National Science & Technology Department.
2-3 anti-fungal disease genes are coinserted with hygromycin phosphotransferase in the same vector. Two insecticidal genes and PPT acetyl transferase genes are placed in another one. The vectors are co-delivered to ri...
关键词:rice BIOLISTIC MULTIPLE GENE transfer ANTI-FUNGAL GENE INSECTICIDAL gene. 
A transgenic wheat with a stilbene synthase gene resistant to powdery mildew obtained by biolistic method被引量:12
《Chinese Science Bulletin》2000年第7期634-638,共5页R.Hain 
Stilbene, a kind of phytoalexin, plays an important role in resistance to fungal and bacterial infection in plants. It strongly inhibits the growth of fungi and sprout of spore. Stilbene synthase gene (Vst1) obtained ...
关键词:wheat transformation by BIOLISTIC METHOD STILBENE SYNTHASE phytoaiexin powdery mildew. 
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