This study presents an optimization of the Folin-Ciocalteu spectrophotometric method for the determination of total phenol content. Multivariate optimization using factorial planning 22 with a central point and centra...
The suitability of the Folin-Ciocalteu reagent (FCR) and of HPLC for analyzing polyphenols is evaluated. FCR assay is commonly used although its flows, such as overestimating polyphenol content due to interference by ...