CHROMOSOME

作品数:645被引量:1467H指数:18
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相关机构:中国农业科学院作物育种栽培研究所中国科学院复旦大学上海交通大学更多>>
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A single circular chromosome yeast被引量:7
《Cell Research》2019年第1期87-89,共3页Yangyang Shao Ning Lu Chen Cai Fan Zhou Shanshan Wang Zhihu Zhao Guoping Zhao Jin-Qiu Zhou Xiaoli Xue Zhongjun Qin 
grants from Chinese Academy of Sciences (XDB19000000);National Natural Science Foundation of China (3183010S, 31770099,31521061);Ministry of Science and Technology (2016YFA0500701).
Dear editor,Most of the prokaryotic cells contain a single circular chromosome.In contrast, the eukaryotic cells usually contain multiple linear chromosomes. Recently, we artificially created a single linear chromosom...
关键词:PROKARYOTIC cells SACCHAROMYCES CEREVISIAE 
Proteasomes on the chromosome
《Cell Research》2017年第5期602-603,共2页Michael Lichten 
Targeted proteolysis plays an important role in the execution and regulation of many cellular events. Two recent papers in Science identify novel roles for proteasome-mediated proteolysis in homologous chromo- some pa...
Painting a specific chromosome with CRISPR/Cas9 for live-cell imaging被引量:7
《Cell Research》2017年第2期298-301,共4页Yuexin Zhou Ping Wang Feng Tian Ge Gao Lei Huang Wensheng Wei X Sunney Xie 
We thank Dr Yujie Sun for providing the dCas9-EGFP expressing plasmid and Dr Ping Wei from the Center of Quantitative Biol- ogy for the Tet-on 3G plasmids. We thank Shaoshuai Meng for his early involvement in this project. We appreciate the assistance of Bo He, Junya Zhao and Zujia Wang from Central South University in karyotype analysis. We are grateful to Xiaochen Li, Xuemei Hao and Chunyan Shan in Peking University imaging user facility for their help of acquiring cell division movies and N-SIM images. We thank Dr Patty Purcell for editing of the manuscript. Meanwhile we appreciate the assistant of Huan Hu for the guidance of FISH protocol. This work is supported by grants from the National Natural Science Foundation of China, NSFC 21390412 and NSFC21327808 for XSX, and NSFC31430025, NSFC31170126, and NSFC81471909 for WW, and in part by a grant from National Basic Research Program of China, 2015AA020407 for LH.
Dear Editor, Visualization of chromosome shapes and dynamics in a live cell is highly desirable and necessary in many areas of cell biology. For example, the copy number of a particular chromosome in cancer cells is o...
关键词:活细胞成像 染色体 动态可视化 细胞生物学 拷贝数 癌细胞 
Kinetochore dynein generates a poleward pulling force to facilitate congression and full chromosome alignment被引量:6
《Cell Research》2007年第8期701-712,共12页Yan Li Wei Yu Yun Liang Xueliang Zhu 
Acknowledgments The authors thank Qiongping Huang, Lirong Liu, and Wei Bian for technical assistance. We are grateful to Drs G Chan (Cross Cancer Institute, University of Alberta, Edmonton Alberta, Canada) for antibodies to human ZW 10 and Rod, KH Choo (Murdoch Children's Research Institute, Royal Children's Hospital, Melbourne, Australia) for anti-CREST serum, and E Fuchs (Rockefeller University, USA) for mRFP cDNA. This work was supported by the National Science Foundation of China (30330330, 30421005, and 30623003), Ministry of Science and Technology of China (2005CB522703 and 2007CB914501), and the Shanghai Municipal Council for Science and Technology (S048014317, 06DZ22032, and 058014578).
For proper chromosome segregation, all kinetochores must achieve bipolar microtubule (MT) attachment and subsequently align at the spindle equator before anaphase onset. The MT minus end-directed motor dynein/dynact...
关键词:KINETOCHORE cytoplasmic dynein CHROMOSOME MITOSIS FORCE 
Characterization of a novel toxin-antitoxin module,VapBC,encoded by Leptospira interrogans chromosome被引量:6
《Cell Research》2004年第3期208-216,共9页YiXuanZHANG XiaoKuiGUO ChuanWU BoBI ShuangXiREN ChunFuWU GuoPingZHAO 
This work was supported by the National High Technology Research and Development Program of China(Program No.2003AA223031).
Comparative genomic analysis of the coding sequences (CDSs) of Leptospira interrogans revealed a pair of closely linked genes homologous to the vapBC loci of many other bacteria with respect to both deduced amino acid...
关键词:Leptospria toxin-antitoxin system VapBC CO-EVOLUTION speciation. 
The ATF/CREB site is the key element for transcription of the human RNA methyltransferase like 1 (RNMTL1) gene, a newly discovered 17p13.3 gene被引量:14
《Cell Research》2002年第4期177-197,共21页JIAN XU JING DE ZHU MIN NI DA FANG WAN JIAN REN GU 
The human RNA methyltransferase like i gene (RNMTL1) is one of thirteen newly discovered geneswithin a 116 Kb segment of the chromosome 17p13.3 that suffers from a high frequent loss of heterozygosityin human hepatoce...
关键词:RNMTL1 gene  ATF/CREB  YY1  TRANSCRIPTION regulation  CHROMOSOME 17p13.3. 
Nuclear assembly of purified Crythecodinium cohnii chromosomes in cell-free extracts of Xenopus laevis eggs
《Cell Research》2000年第2期127-137,共11页LIU XIAO LING YAN SHEN ER JIE CHEN ZHONG HEZHAI(E-mail: zhaizh@plum.lsc.pku.edu.cn Tel: 86-10-62754293 Fax: 86-10-62751526)(College of Life Sciences, Peking University, Beijing 100871,China) 
Incubation of dinoflagellate Crythecodinium cohnii chromosomes in cytoplasmic extracts of unfertilized Xenopus laevis eggs resulted in chromosomes decondensation and recondensation, nuclear envelope assembly, and nucl...
关键词:Dinoflagellate Crythecodinium cohnii CHROMOSOME nuclear reconstitution cell-free system Xenopus laevis egg extracts 
Tropomyosin is localized in the nuclear matrix and chromosome scaffold of physarum polycephalum被引量:1
《Cell Research》1999年第1期61-69,共9页ZENG XIAN LU MING DA JIAO MIAO XING XIAO GUANG WANG SHUI HAO(Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China)(Department of Biology, Changchun Normal College,Changchun 130032, China) 
The nuclei and chromosomes were isolated from plasmodia of Physarum polycephalum. The nuclear matrir and chromosome scaffold were obtained after the DNA and most of the proteins were extracted with DNase I and 2 M NaC...
关键词:Chromosome scaffold nuclear matrix Physarum polycephalum TROPOMYOSIN 
Construction of a DNA library from chromosome 4 of rice(Oryza sativa)by microdissection被引量:2
《Cell Research》1998年第4期285-293,共9页MAO YING WEI SI YUAN LIANG WEN QIN SONG XIU LAN LI RUI YANG CHEN (Biology Department, Nankai University, Tianjin 300071,China) 
A simple method to create a chromosome-specific DNA library of rice, including microdissection, amplification,characterization and cloning, is described. Rice chromosome 4 from a metaphase cell has been isolated and a...
关键词:Rice chromosome 4 DNA library MICRODISSECTION LA-PCR 
Isolation of 24 novel cDNA fragments from microdissected human chromosome band
《Cell Research》1998年第2期135-142,共8页ZHANG MIN L0NG YU PEI RONG HU AN DINGBI JIA HUI XIA HAN XIANG DENG SHOU YUANZHAO(Institute 0f Genetics, Fudan University, Shanghai 2O0j23,China(State Key La6omtory of Medical Genetics, Hunan Medical Un1versity, Chang8ha J10078, China) 
The strategy of isolating the band-specific expression fragments from a probe pool generated by humanchromosome microdissection was reported. A cliromosome 14q24.3 band-specific single copy DNA pool was constructed ba...
关键词:Probe pool chromosome 14q24.3 single copy DNA pool expression sequences of genes 
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