grants from Chinese Academy of Sciences (XDB19000000);National Natural Science Foundation of China (3183010S, 31770099,31521061);Ministry of Science and Technology (2016YFA0500701).
Dear editor,Most of the prokaryotic cells contain a single circular chromosome.In contrast, the eukaryotic cells usually contain multiple linear chromosomes. Recently, we artificially created a single linear chromosom...
Targeted proteolysis plays an important role in the execution and regulation of many cellular events. Two recent papers in Science identify novel roles for proteasome-mediated proteolysis in homologous chromo- some pa...
We thank Dr Yujie Sun for providing the dCas9-EGFP expressing plasmid and Dr Ping Wei from the Center of Quantitative Biol- ogy for the Tet-on 3G plasmids. We thank Shaoshuai Meng for his early involvement in this project. We appreciate the assistance of Bo He, Junya Zhao and Zujia Wang from Central South University in karyotype analysis. We are grateful to Xiaochen Li, Xuemei Hao and Chunyan Shan in Peking University imaging user facility for their help of acquiring cell division movies and N-SIM images. We thank Dr Patty Purcell for editing of the manuscript. Meanwhile we appreciate the assistant of Huan Hu for the guidance of FISH protocol. This work is supported by grants from the National Natural Science Foundation of China, NSFC 21390412 and NSFC21327808 for XSX, and NSFC31430025, NSFC31170126, and NSFC81471909 for WW, and in part by a grant from National Basic Research Program of China, 2015AA020407 for LH.
Dear Editor, Visualization of chromosome shapes and dynamics in a live cell is highly desirable and necessary in many areas of cell biology. For example, the copy number of a particular chromosome in cancer cells is o...
Acknowledgments The authors thank Qiongping Huang, Lirong Liu, and Wei Bian for technical assistance. We are grateful to Drs G Chan (Cross Cancer Institute, University of Alberta, Edmonton Alberta, Canada) for antibodies to human ZW 10 and Rod, KH Choo (Murdoch Children's Research Institute, Royal Children's Hospital, Melbourne, Australia) for anti-CREST serum, and E Fuchs (Rockefeller University, USA) for mRFP cDNA. This work was supported by the National Science Foundation of China (30330330, 30421005, and 30623003), Ministry of Science and Technology of China (2005CB522703 and 2007CB914501), and the Shanghai Municipal Council for Science and Technology (S048014317, 06DZ22032, and 058014578).
For proper chromosome segregation, all kinetochores must achieve bipolar microtubule (MT) attachment and subsequently align at the spindle equator before anaphase onset. The MT minus end-directed motor dynein/dynact...
This work was supported by the National High Technology Research and Development Program of China(Program No.2003AA223031).
Comparative genomic analysis of the coding sequences (CDSs) of Leptospira interrogans revealed a pair of closely linked genes homologous to the vapBC loci of many other bacteria with respect to both deduced amino acid...
The human RNA methyltransferase like i gene (RNMTL1) is one of thirteen newly discovered geneswithin a 116 Kb segment of the chromosome 17p13.3 that suffers from a high frequent loss of heterozygosityin human hepatoce...
Incubation of dinoflagellate Crythecodinium cohnii chromosomes in cytoplasmic extracts of unfertilized Xenopus laevis eggs resulted in chromosomes decondensation and recondensation, nuclear envelope assembly, and nucl...
The nuclei and chromosomes were isolated from plasmodia of Physarum polycephalum. The nuclear matrir and chromosome scaffold were obtained after the DNA and most of the proteins were extracted with DNase I and 2 M NaC...
A simple method to create a chromosome-specific DNA library of rice, including microdissection, amplification,characterization and cloning, is described. Rice chromosome 4 from a metaphase cell has been isolated and a...
The strategy of isolating the band-specific expression fragments from a probe pool generated by humanchromosome microdissection was reported. A cliromosome 14q24.3 band-specific single copy DNA pool was constructed ba...