GREEN_FLUORESCENT_PROTEIN

作品数:97被引量:212H指数:8
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相关作者:丘志平魏燕燕王凌健叶永健陈晶更多>>
相关机构:华中科技大学第四军医大学香港大学中国科学院更多>>
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Stable plastid transformation in kiwifruit(Actinidia chinensis)
《aBIOTECH》2025年第1期72-80,共9页Qiqi Chen Yuyong Wu Yanchang Wang Jiang Zhang Shengchun Li 
supported by grants from the National Natural Science Foundation of China(32271912,32071477).
Plastid transformation offers valuable benefits in plant biotechnology,such as high-level transgene expression and the absence of gene silencing.Here we describe the first protocol of a plastid transformation system f...
关键词:Actinidia chinensis Woody vine Transplastomic technology aadA Green fluorescent protein 
Visualizing Wallerian degeneration in the corticospinal tract after sensorimotor cortex ischemia in mice被引量:1
《Neural Regeneration Research》2024年第3期636-641,共6页Jiao Mu Liufang Hao Zijue Wang Xuyang Fu Yusen Li Fei Hao Hongmei Duan Zhaoyang Yang Xiaoguang Li 
supported by the National Natural Science Foundation of China,Nos.31 730030 (to XL),81941011 (to XL),31 771053 (to HD),82271403 (to XL),82272171 (to ZY),31971279 (to ZY);82201542 (to FH);the Natural Science Foundation of Beijing,No.7222004 (to HD);the Science and Technology Program of Beijing,No.Z181100001818007(to ZY)
Stroke can cause Wallerian degeneration in regions outside of the brain,particularly in the corticospinal tract.To investigate the fate of major glial cells and axons within affected areas of the corticospinal tract f...
关键词:corticospinal tract green fluorescent protein MICROGLIA spinal anterior horn cells stroke virus trace Wallerian degeneration 
Bioinspired Fluorescent Polymers: Synthesis, Processing, and Applications
《Chinese Journal of Chemistry》2023年第4期458-468,共11页Hongxue Xu Wei Zhang Mingfeng Wang 
We acknowledge the financial support by the University Development Fund-Research Start-up Fund(UDF01001806)from the Chinese University of Hong Kong,Shenzhen.H.X.and W.Z.thank the financial support of Ph.D.Scholarship from the Chinese University of Hong Kong,Shenzhen.
The discovery of fluorescent proteins has inspired a lot of interest to develop synthetic polymers with similar structures and functionalities.In this review article,we first give a brief introduction for the discover...
关键词:Green fluorescent protein FLUORESCENCE PROTEINS CHROMOPHORES POLYMERS 
Colonization of Penicillium oxalicum SL2 in Pb-contaminated paddy soil and its immobilization effect on soil Pb被引量:1
《Journal of Environmental Sciences》2022年第10期53-62,共10页Yu Gao Xiaohan Jiang Hanxin Wu Jianhao Tong Xinyue Ren Jiayu Ren Qianhua Wu Jien Ye Chunhui Li Jiyan Shi 
supported by the National Natural Science Foundation of China (No. 42077119);the Innovative Research Group Project of the National Natural Science Foundation of China (No. 41721001)。
Penicillium oxalicum SL2(SL2) is a previously screened Pb-tolerant fungus that can promote crops growth. The relationship between SL2 colonization and Pb immobilization was studied to provide a theoretical basis for m...
关键词:Penicillium oxalicum SL2 COLONIZATION Lead Availability Green fluorescent protein 
A preparation strategy for protein-oriented immobilized silica magnetic beads with Spy chemistry for ligand fishing被引量:1
《Journal of Pharmaceutical Analysis》2022年第3期415-423,共9页Yu Yi Jianming Hu Shenwei Ding Jianfeng Mei Xudong Wang Yanlu Zhang Jianshu Chen Guoqing Ying 
supported by the Zhejiang Foundation Public Welfare Research Project(Authorization No.:LGF19B060006)。
Due to the complexity of bioactive ingredients in biological samples,the screening of target proteins is a complex process.Herein,a feasible strategy for directing protein immobilization on silica magnetic beads for l...
关键词:EDC/Sulfo-NHS SpyTag/SpyCatcher Green fluorescent protein Silica magnetic beads Oriented immobilization Drug screening 
Imidazolium-based ionic liquids as additives to preserve the Enhanced Green Fluorescent Protein fluorescent activity被引量:1
《Green Chemical Engineering》2021年第4期412-422,共11页Nathalia V.Veríssimo Carolina F.Saponi Timothy M.Ryan Tamar L.Greaves Jorge F.B.Pereira 
the project“Understanding the molecular interactions between ionic liquids and biopharmaceuticals:the key for a proper design of downstream processing”,2018/50009–8,co-funded by FAPESP(S~ao Paulo Research Foundation,Brazil)and Australian Technology Network of Universities(ATN)and projects FAPESP 2014/19793–3,2014/16424–7 and 2018/25511–1.N.V.Veríssimo(2016/07529–5,2018/06576–5,and 2020/14144–8);C.F.Saponi(2018/01858–2 and 2018/20833–0)acknowledge scholarships financial support from FAPESP.N.V.Veríssimo,J.F.B.Pereira also acknowledge the funding from CNPq,CAPES(001),CAPES-PROEX.;CIEPQPF is supported by the FCT through the projects UIDB/EQU/00102/2020 and UIDP/EQU/00102/2020.
Fluorescent Proteins(FP)can be applied as biomarkers and biosensors in the industrial and medical fields,but their large-scale use,especially for new industrial applications,is limited due to their low stability.Hence...
关键词:Green Fluorescent Protein Protein stability Fluorescence Ionic liquids PRESERVATIVES 
Inhibition of Botrytis cinerea and control of gray mold on table grapes by calcium propionate被引量:1
《Food Quality and Safety》2021年第3期279-290,共12页Chenxu Sun Chuanxi Zhu Ying Tang Dandan Ren Yunfei Cai Ganghan Zhou Yiwen Wang Ling Xu Pinkuan Zhu 
supported by the grants from Shanghai Municipal Science and Technology Commission,China(No.18391901400);the National Key Research and Development Program of China(2016YFD0400105);the National Natural Science Foundation of China(No.32061133006);the Shanghai Municipal Agricultural and Rural Committee(2019-02-08-00-02-F0U46),China。
Objectives:The gray mold fungus Botrytis cinerea(B.cinerea)infects a wide range of crops before and after harvest,causing huge losses worldwide.Inhibition mechanisms of B.cinerea in vitro and in plants by calcium prop...
关键词:Necrotrophic pathogen virulence factor infection cushion green fluorescent protein generally recognized as safe. 
Synthesis and Photophysical Properties of Conjugates of Green Fluorescent Protein (GFP) Chromophore and 2'-Deoxy-Uridine Developed as Labelled Building Blocks for Oligonucleotide Probes
《Journal of Pharmacy and Pharmacology》2019年第9期510-519,共10页Abed Saady Bilha Fischer 
Fluorescent probes with high signal/background ratio are needed for hybridization assays. Hence, the chromophore of green fluorescent protein (GFP), 4-hydroxybenzylidene-imidazolinone (HBI), was targeted as a potentia...
关键词:INTERCALATOR GFP 2'-deoxyuridine INTERCALATOR CONJUGATES 
Synthesis and characteristic of a novel green fluorescent protein eYGFPuv被引量:1
《Oil Crop Science》2019年第2期65-74,共10页Shihang Fan Linbin Deng Hongli Yang Liang Zhang Xingchao Sun Jinglin Liu Jing Liu 
supported by the National Natural Science Foundation of China (31500237)
Recently, a novel green fluorescent protein eYGFPuv has been identified in the marine organism Chiridius poppei which displays high fluorescence intensity and can be visible by eyes in dark. Although strong green fluo...
关键词:eYGFPuv green FLUORESCENT PROTEIN SCREENING MARKER 
A Viral Expression Vector from Foxtail mosaic virus to Express Green Fluorescent Protein
《Agricultural Science & Technology》2019年第2期42-47,共6页CHEN You-qian WU Juan ZHU Pin LI Xiang ZHU Xi-wu 
Supported by Key Laboratory Open Foundation Project of Hunan Education Department(18K100);Graduate Research Innovation Project of Hunan Province(CX2018B800)~~
[Objective]Foxtail mosaic virus(FoMV)infects gramineous and dicotyledonous plants.In this study,we sought to construct a viral vector based on FoMV to express exogenous proteins in plants.[Method]A recombinant viral e...
关键词:Foxtail mosaic virus Recombinant viral expression vector Green fluorescent protein Exogenous gene sequences Nicotiana benthamiana 
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