MAMMALIAN_CELLS

作品数：89被引量：390H指数：7
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Atomic force microscopy imaging of live mammalian cells被引量：6
《Science China(Life Sciences)》2013年第9期811-817,共7页LI Mi LIU LianQing XI Ning WANG YueChao DONG ZaiLi XIAO XiuBin ZHANG WeiJing 
supported by the National Natural Science Foundation of China (61175103);CAS FEA International Partnership Program for Creative Research Teams
Atomic force microscopy (AFM) was used to examine the morphology of live mammalian adherent and suspended cells. Time-lapse AFM was used to record the locomotion dynamics of MCF-7 and Neuro-2a cells. When a MCF-7 cell...
关键词：atomic force microscopy cell locomotion LAMELLIPODIA CYTOSKELETON POLYDIMETHYLSILOXANE 
Microscale methods to assemble mammalian cells into tissue-like structures被引量：1
《Science China(Life Sciences)》2012年第10期862-871,共10页GONG PeiYuan ZHENG WenFu XIAO Dan JIANG XingYu 
supported by Ministry of Science and Technology of China(Grant Nos.2009CB930001 and 2011CB933201);Chinese Academy ofSciences(Grant No.KJCX2-YW-M15);the National Natural ScienceFoundation of China(Grant Nos.20890020,90813032,21025520 and 51073045)
Different cell types make up tissues and organs hierarchically and communicate within a complex, three-dimensional (3D) en- vironment. The in vitro recapitulation of tissue-like structures is meaningful, not only for ...
关键词：microscale cell assembly tissue-like structures tissue engineering 
GroupⅠbut not GroupⅡNPV induces antiviral effects in mammalian cells被引量：1
《Science China(Life Sciences)》2006年第5期467-472,共6页LIANG Changyong1,2,SONG Jianhua1,2,HU Zhihong1 & CHEN Xinwen1 1.State Key Laboratory of Virology,Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan 430071,China 2.Graduate School of the Chinese Academy of Sciences,Beijing 100039,China. 
supported by the National Natural Science Foundation of China(Grant Nos.30325002,30470075);National Basic Research Priorities Program of China(Grant No.2003CB1140).
Nucleopolyhedrovirus(NPV) is divided into Group I and Group II based on the phy-logenetic analysis.It has been reported that Group I NPVs such as Autographa californica multiple NPV(AcMNPV) can transduce mammalian cel...
关键词：antiviral effect  baculovirus  VSV  IFN-α/β  mammalian cells  TRANSGENE expression. 
Baculovirus-mediated gene transfer into mammalian cells
《Science China(Life Sciences)》1998年第5期473-478,共6页朱应 齐义鹏 刘子夜 肖庚富 
A kind of cytotoxinic gene barnase was cloned downstream of immediate early promoter from cytomegalovirus (CMV). Recombinant virus vAcCMVBar was constructed using Bac\|to\|Bac system by insertion of the expression cas...
关键词：ACNPV BARNASE gene MAMMALIAN cells. 
Expression of structural proteins of hepatitis C virus (HCV) in mammalian cells被引量：3
《Science China(Life Sciences)》1998年第1期47-55,共9页李迎春 李光地 孔玉英 汪垣 王宇 闻玉梅 
ProjectsupportedbytheNational"863"HighTechnologyFoundationofChina.
The vaccinia viral vector containing T7 promoter was used to construct the expression plasmids carrying HCV structural genes of C, E1 and E2/NS1. These genes were transiently expressed in mammalian cells in the presen...
关键词：hepatitis C VIRUS core PROTEIN envelope PROTEIN recombinant VACCINIA VIRUS transient EXPRESSION immunoblotting. 
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