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CELL-FREE: 作品数：148被引量：425H指数：12; 导出分析报告; 相关作者：冉静学孙强李秀军郭晓东侯朝晖更多>>; 相关机构：哈尔滨工业大学中国航天科工集团第四研究院北京机电工程总体设计部中央民族大学凯迪迪爱通信技术有限公司更多>>; 相关期刊：更多>>; 相关基金：国家自然科学基金国家重点基础研究发展计划国家高技术研究发展计划北京市自然科学基金更多>>

Promoting the production of challenging proteins via induced expression in CHO cells and modified cell-free lysates harboring T7 RNA polymerase and mutant eIF2α: 《Synthetic and Systems Biotechnology》2024年第3期416-424,共9页Jeffrey L.Schloßhauer Lena Tholen Alexander Korner Stefan Kubick Sofia Chatzopoulou Anja Honow Anne Zemella; supported by the European Regional Development Fund(EFRE)and the German Ministry of Education and Research(BMBF 031B0831C).; Chinese hamster ovary(CHO)cells are crucial in biopharmaceutical production due to their scalability and capacity for human-like post-translational modifications.However,toxic proteins and membrane proteins are often ...; 关键词：Inducible expression CHO cells Cell-free protein synthesis CRISPR T7 RNA polymerase eIF2 Rosa26

Constructing an artificial short route for cell-free biosynthesis of the phenethylisoquinoline scaffold: 《Synthetic and Systems Biotechnology》2023年第4期610-617,共8页Yuhao Zhang Wan-Qiu Liu Jian Li; supported by grants from the National Natural Science Foundation of China(32171427 and 31971348)。; Plant-originated natural products are important drug sources.However,total biosynthesis of these compounds is often not achievable due to their uncharacterized,lengthy biosynthetic pathways.In nature,phenethylisoquino...; 关键词：Phenethylisoquinoline scaffold Artificial pathway Cell-free biosynthesis Natural product

Xenopus cell-free extracts and their applications in cell biology study: 《Biophysics Reports》2023年第4期195-205,共11页Junjun Liu Chuanmao Zhang; supported by a grant from NIH to J.L(1 SC3 GM132056);by grants from National Natural Science Foundation of China to CMZ(32130026 and 92254305); Xenopus has proven to be a remarkably versatile model organism in the realm of biological research for numerous years,owing to its straightforward maintenance in laboratory settings and its abundant provision of ample...; 关键词：XENOPUS Cell-free extract OOCYTE Egg EMBRYO MEIOSIS MITOSIS Spindle

Corrigendum to“Aptamer-based cell-free detection system to detect target protein”[Synth Syst Biotechnol 6(3)(2021)209–215]: 《Synthetic and Systems Biotechnology》2023年第2期339-339,共1页Junhong Chen Xiaoyan Zhuang Jiyang Zheng Ruofan Yang Fei Wu Aihui Zhang Baishan Fang; The authors regret that following the publication of the original article,the authors noticed that the“Affiliation a”were incorrect.It should be“XMU-China Team,College of Chemistry and Chemical Engineering,Xiamen U...; 关键词：XIAMEN noticed (3)

Customized synthesis of phosphoprotein bearing phosphoserine or its nonhydrolyzable analog: 《Synthetic and Systems Biotechnology》2023年第1期69-78,共10页Dong Liu Yingying Liu Hua-Zhen Duan Xinjie Chen Yanan Wang Ting Wang Qing Yu Yong-Xiang Chen Yuan Lu; supported by the National Natural Science Foundation of China (21878173,22177059);National Key R&D Program of China (2018YFA0901700,2021YFC2103900);a grant from the Institute Guo Qiang,Tsinghua University (2021GQG1016).; Studies on the mechanism of protein phosphorylation and therapeutic interventions of its related molecular processes are limited by the difficulty in the production of purpose-built phosphoproteins harboring site-spec...; 关键词：Cell-free synthetic biology PHOSPHOPROTEIN PHOSPHORYLATION Orthogonal translation system Nonhydrolyzable analog

Aptamer-based cell-free detection system to detect target protein被引量：1: 《Synthetic and Systems Biotechnology》2021年第3期209-215,共7页Junhong Chen Xiaoyan Zhuang Jiyang Zheng Ruofan Yang Fei Wu Aihui Zhang Baishan Fang; financially supported by the National Natural Science Foundation of China(No.21978245)。; Biomarkers of disease,especially protein,show great potential for diagnosis and prognosis.For detecting a certain protein,a binding assay implementing antibodies is commonly performed.However,antibodies are not therma...; 关键词：APTAMER CRISPR-Cas Protein detection BIOMARKER

Methodologies for preparation of prokaryotic extracts for cell-free expression systems被引量：1: 《Synthetic and Systems Biotechnology》2020年第4期252-267,共16页Stephanie D.Cole Aleksandr E.Miklos Abel C.Chiao Zachary Z.Sun Matthew W.Lux; Cell-free systems that mimic essential cell functions,such as gene expression,have dramatically expanded in recent years,both in terms of applications and widespread adoption.Here we provide a review of cell-extract m...; 关键词：Cell-free systems Cell-free expression Cell-free extract Synthetic biology Methods

Cell-free synthetic biology in the new era of enzyme engineering被引量：1: 《Chinese Journal of Chemical Engineering》2020年第11期2810-2816,共7页Nan Jiang Lianju Ma Yuan Lu; supported by the National Key R&D Program of China(2018YFA0901700);the National Natural Science Foundation of China(Nos.21878173,21706144);the Natural Science Foundation of Beijing City(2192023)。; With the gradual rise of enzyme engineering,it has played an essential role in synthetic biology,medicine,and biomanufacturing.However,due to the limitation of the cell membrane,the complexity of cellular metabolism,t...; 关键词：Cell-free synthetic biology Enzyme engineering Metabolic engineering

Preparation of biomimetic gene hydrogel via polymerase chain reaction for cell-free protein expression被引量：1: 《Science China Chemistry》2020年第1期99-106,共8页Feng Li Wenting Yu Xue Zhang Xiaocui Guo Xihan Xu Xiaolei Sun Dayong Yang; supported by the National Natural Science Foundation of China(21621004,21575101,21622404); Deoxyribonucleic acid(DNA)hydrogels,a three-dimensional(3 D)network made from DNA chains,have attracted great attention because of its molecular programmability,excellent biocompatibility and wide biomedical applicati...; 关键词：HYDROGEL DNA polymerase chain reaction free radical polymerization biomimetic materials

Conversion of D-glucose to L-lactate via pyruvate by an optimized cell-free enzymatic biosystem containing minimized reactions被引量：2: 《Synthetic and Systems Biotechnology》2018年第3期204-210,共7页Leipeng Xie Xinlei Wei Xigui Zhou Dongdong Meng Ruimin Zhou Yi-Heng P.Job Zhang Shuxia Xu Chun You; the Key Research Program of the Chinese Academy of Sciences(Grant No.ZDRW-ZS-2016-3);National Natural Science Foundation of China(Grant No.31600636).; Cell-free synthetic enzymatic biosystem is emerging to expand the traditional biotechnological mode by utilizing a number of purified/partially purified enzymes and coenzymes in a single reaction vessel for the produc...; 关键词：Cell-free synthetic enzymatic biosystem Minimized reactions Thermophilic enzymes L-LACTATE PYRUVATE Biomanufacturing

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