[Objective] To explore the efficient way for the preparation of Aspergillus oryzae protoplast.[Method] The statistics of protoplast yield by the enzymolysis of mycelia with different culture time,different osmotic sta...
the National Science-Technology Supporting Project for the 11th Five-Year Plan (Nos. 2006BAD27B09-6 and 2007BAK36B03);the Key Technology R&D Program of Guangdong Province (Nos. 2007A010900001 and 2008A010900001)
This work was supported by a scholarship provided by Japan International Cooperation Agency(JICA) .The study was done in the National Institute of Brewing(Hiroshima;Japan)
Aminoacylase was immobilized on the mycelium cells of Aspergillus oryzae by using ethylenediamine, gelatin and glutylalhyde. The proper immobilized condition was studied by orthogonal experimental design. The immo...