SPERM

作品数:604被引量:1404H指数:16
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A paternal protein facilitates sperm RNA delivery to regulate zygotic development
《Science China(Life Sciences)》2023年第10期2342-2353,共12页Dongdong Li Shijing Huang Yongping Chai Ruiqian Zhao Jing Gong Qiangfeng Cliff Zhang Guangshuo Ou Wenyu Wen 
the Ministry of Science and Technology of China(2019YFA0508401);the National Natural Science Foundation of China(31871394,82121004,32100538);Shanghai Municipal Science and Technology Major Project(2018SHZDZX01);the China Postdoctoral Science Foundation(2022M711844);the Young Elite Scientists Sponsorship Program by CAST(YESS20220102);ZJ Lab and Shanghai Center for Brain Science and Brain-Inspired Technology。
Sperm contributes essential paternal factors,including the paternal genome,centrosome,and oocyte-activation signals,to sexual reproduction.However,it remains unresolved how sperm contributes its RNA molecules to regul...
关键词:SPE-11 phase separation paternal factor early embryonic development 
Surrogate production of genome-edited sperm from a different subfamily by spermatogonial stem cell transplantation被引量:13
《Science China(Life Sciences)》2022年第5期969-987,共19页Fenghua Zhang Yongkang Hao Xianmei Li Yi Li Ding Ye Ru Zhang Xiaosi Wang Mudan He Houpeng Wang Zuoyan Zhu Yonghua Sun 
supported by the National Natural Science Foundation of China(32025037 and 31721005);the National Key R&D Project of China(2018YFA0801000 and 2018YFD0901205);Chinese Academy of Sciences(XDA24010108);State Key Laboratory of Freshwater Ecology and Biotechnology(2019FBZ05)。
The surrogate reproduction technique,such as inter-specific spermatogonial stem cells(SSCs)transplantation(SSCT),provides a powerful tool for production of gametes derived from endangered species or those with desirab...
关键词:surrogate reproduction genome editing spermatogonial stem cell transplantation ZEBRAFISH Gobiocypris rarus 
GPS in pollen tubes: Found!
《Science China(Life Sciences)》2016年第4期438-439,共2页Sha Li Yan Zhang 
Sexual reproduction of flowering plants depends on the delivery of two immotile sperm cells to the female gametophyte(FG),i.e.the embryo sac,through the growth of a pollen tube,a long cylindrical cellular extension ...
关键词:pollen embryo fertilization Recognition flowering sperm journey directional reproduction toward 
Epigenetic reprogramming of embryos derived from sperm frozen at-20℃被引量:5
《Science China(Life Sciences)》2012年第4期349-357,共9页CHAO ShiBin LI JianChun JIN XuanJin TANG HaiXun WANG GongXian GAO GuoLan 
supported by the National Basic Research Program of China (Grant No. 2011CB944501)
Cryopreservation of spermatozoa is a strategy that has been used to conserve the sperm of animal species and animal strains that are valuable for biomedical research. A simple method for preserving spermatozoa after a...
关键词:spermatozoa freezing ICSI EPIGENETIC histone modification DNA methylation 
Lipid metabolism and Drosophila sperm development被引量:4
《Science China(Life Sciences)》2012年第1期35-40,共6页WANG Chao HUANG Xun 
supported by the National Natural Science Foundation of China (Grant Nos. 30771060 and 31071253)
Lipids are essential membrane structural components and important signal carriers. The major enzymatic metabolisms of various lipids (phospholipid, sphingolipid, cholesterol) are well studied. The developmental functi...
关键词:Drosophila spermatogenesis phosphatidylinositol lipids fatty acids CHOLESTEROL 
What we have learned from transcript profile analyses of male and female gametes in flowering plants被引量:1
《Science China(Life Sciences)》2010年第8期927-933,共7页XIN HaiPing & SUN MengXiang Key Laboratory of the Ministry of Education for Plant Developmental Biology, College of Life Science, Wuhan University, Wuhan 430072, China 
supported by the National Basic Research Program of China (Grant No. 2007CB108700);the National Natural Science Foundation of China (Grant Nos. 30821064 and 30771131)
Double fertilization is one of the predominant features of sexual reproduction in flowering plants but, because of the physical inaccessibility of gametes, the essential molecular mechanisms in these processes are lar...
关键词:SPERM CELL egg CELL fertilization cDNA library GAMETOGENESIS TRANSCRIPT PROFILE 
Construction of sperm-specific lactate dehydrogenase DNA vaccine and experimental study of its immuno-contraceptive effect on mice被引量:3
《Science China(Life Sciences)》2008年第4期308-316,共9页CHEN Yong ZHANG Duo XIN Na XIONG YongZhong CHEN Ping LI Bo TU XiangDong LAN FengHua 
Grants from the Key Research Projects of Science and Technology of Fujian Province,China (Grant No. 2002Y032);Chinese PLA Foundation of Medical Sciences during the Tenth Five–Year Period (Grant No. 01MA032)
Lactate dehydrogenase C4 (LDHC4) is a key enzyme for sperm metabolism. It is distributed specifically in testis and is highly immunogenic. In this study, two DNA vaccines pVAX1-hLDHC and pVAX1-mLDHC were constructed b...
关键词:sperm-specific lactate dehydrogenase DNA vaccine contraceptive vaccine mucosal immunization 
The formation of improved tetraploid population of red crucian carp×common carp hybrids by androgenesis被引量:4
《Science China(Life Sciences)》2007年第6期753-761,共9页DUAN Wei QIN QinBo CHEN Song LIU ShaoJun WANG Jing ZHANG Chun SUN YuanDong LIU Yun 
Supported by the National Natural Science Foundation of China(Grant Nos.30330480 and 30571444);the Doctoral Station of University of the Education Min-istry of China(Grant No.200405422001);the Agriculture Technological Production Foundation of the Science and Technology Ministry of China(Grant No.2005380);Important Solution Project of Hunan Province(Grant No.2006NK2008)
Bisexual fertile diploid androgenetic individuals(A_(0))(2n=100)were formed by androgenesis.In this way,the diploid spermatozoa from male allotetraploid hybrids(AT)(4n=200)of red crucian carp(Carassius auratus red var...
关键词:allotetraploid hybrid diploid sperm haploid egg rogenesis DIPLOID rogenetic individuals 
A rhizobia strain isolated from root nodule of gymno-sperm Podocarpus macrophyllus被引量:3
《Science China(Life Sciences)》2007年第2期228-233,共6页HUANG BaoLing, LU ChengQun, WU Bo & FAN LiQin 1 Forestry College, Guangxi University, Nanning 530004, China 2 Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization, Nanning 530004, China 
the National Natural Science Foundation of China(GrantNo.30170033)
Symbiotic nitrogen fixation of rhizobia and leguminous plants is considered as the most important biologic nitrogen fixation system on earth. Symbiotic nodulation of gymnosperm Podocarpus macro-phyllus and rhizobia ha...
关键词:PODOCARPUS macrophyllus P. macrophyllus VAR. maki RHIZOBIA PHYSIOLOGICAL and BIOCHEMICAL characteristics 16S rDNA sequence 
Nuclear reconstitution of demembranated Orychophragmus violaceus sperm in Xenopus laevis egg extracts
《Science China(Life Sciences)》2002年第6期623-630,共8页卢萍 任民 翟中和 
This work was supported by the National Natural Science Foundation of China (Grant Nos. 19890380 & 30070388) ; the Special Funds for Major State Basic Research of China
The cell-free extracts from animal Xenopus laevis egg could induce chromatin decon- densation and pronuclear formation from demembranated plant (Orychophragmus violaceus)sperm. The demembranated Orychophragmus violace...
关键词:CELL-FREE system  nuclear reconstitution  Orychophragmus violaceus  demembranated sperm  XENOPUS laevis  egg extracts. 
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